Ketanserin

The larvae of M. separata were reared on an artificial diet in the climate incubator and maintained under a photoperiod of 14:10 h (light:dark) at 27 °C and 75% relative humidity [58 ]. Adult moths were provided with 10 % (weight by volume) sucrose solution. Various tissues from fifth instar larvae, including heads, epidermis, fat bodies, Malpighian tubules, and guts, were immediately dissected, and hemocytes were collected by cutting the thoracic legs. Various tissues from three day-old unmated female or male moths including brains, thoraxes, abdomens, antennae, labial palps, wings, and legs were collected, and all samples were used for RNA extraction. The reagents used in the pharmacological ligands, including serotonin hydrochloride (5-HT), (±)-octopamine hydrochloride (OA), dopamine hydrochloride (DA), tyramine hydrochloride (TA), (±)-8-hydroxy-2-(dipropylamino) tetralin hydrobromide (8-OH-DPAT), forskolin, 5-methoxytryptamine (5-MT), methiothepin mesylate, α-methylserotonin maleate (αm-5-HT), WAY-100635, SB-258719, and SB-269970 hydrochloride were purchased from Sigma-Aldrich (St Louis, MO, USA). Ketanserin was purchased from MedChemExpress (Monmouth Junction, NJ, USA).