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2 protocols using isorhamnetin

1

Phytochemical Anti-Inflammatory Screening

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Isoliquiritigenin, isorhamnetin and quercetin (≥98% purity by HPLC) was obtained from Chengdu Pufei De Biotech Co., Ltd (Chengdu, China). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco (Grand Island, USA). Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich Co., Ltd (St Louis, USA).
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2

HPLC Analysis of Bioactive Compounds

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Six commercial standards of HPLC grade, including catechins, chlorogenic acid, hyperoside, quercetin, kaempferol, and isorhamnetin, were purchased from Chengdu Pufei De Biotech Co., Ltd. (China). Other HPLC grade reagents used were acetonitrile and methanol from Thermo Fisher Scientific Co., Ltd. (USA). The Watsons distilled water was applied to prepare for samples and the mobile phase. All other reagents were just met for an analytical grade.
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