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Apc conjugated anti cd11b monoclonal antibody

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The APC-conjugated anti-CD11b monoclonal antibody is a laboratory reagent used for the detection and analysis of CD11b-expressing cells, such as monocytes and macrophages, in various research applications. The antibody is conjugated with the fluorescent dye Allophycocyanin (APC) to enable flow cytometric detection and quantification of CD11b-positive cells.

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Lab products found in correlation

Facscalibur, by BD (2 mentions) Alexa fluor 555 goat anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions) Ab20669, by Abcam (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Aria plus high speed sorter, by BD (1 mentions)

Spelling variants of this product

CD11b-APC (74 citations) Anti-CD11b-APC (38 citations) APC-conjugated anti-CD11b (12 citations) Anti-CD11b antibody (10 citations) CD11b antibody (10 citations) APC)-conjugated anti-CD11b antibody (3 citations) APC-conjugated CD11b antibody (3 citations) Anti-CD11b monoclonal antibody (2 citations) CD11b APC antibody (2 citations) Monoclonal antibody CD11b (2 citations)

4 protocols using apc conjugated anti cd11b monoclonal antibody

1

Mitigating Hematopoietic Differentiation via miR-193a

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To determine the effect of miR-193a on the function of stromal cells to support hematopoietic precursor cells in their primitive state, we first plated HS27A-193a or HS27A-Control cells 2 ml media (105 each well) in six-well adherent plates. Sixteen hours later, 105 U937-Notch1 (or control U937-pCDNA) were suspended in 0.5 ml of media and added to the adherent cells. All Trans-Retinoic Acid (ATRA) was added at 25 µM final concentration to induce differentiation. Ninety-six hours after coculture, all cells (adherent and nonadherent) were harvested by EDTA treatment and stained with APC-conjugated anti-CD11b monoclonal antibody (BD Biosciences) or appropriate isotype control. Fluorescence-activated cell sorting (FACS) analysis was performed on a FACS-Calibur on the U937 cells population by excluding the HS27A cells by GFP positivity.
Balakrishnan I., Yang X., Brown J., Ramakrishnan A., Torok–Storb B., Kabos P., Hesselberth J.R, & Pillai M.M. (2014). Genome-Wide Analysis of miRNA-mRNA Interactions in Marrow Stromal Cells. Stem cells (Dayton, Ohio), 32(3), 662-673.
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2

Flow Cytometric Analysis of CD11b Expression

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For flow cytometry, cells were fixed with 4 % paraformaldehyde in PBS for 10 min and then blocked with 1 % BSA in PBS for 10 min at room temperature. Fixed, blocked cells were incubated with APC-conjugated anti-CD11b monoclonal antibody (BD PharMingen) for 15 min on ice, and cells were washed 3 times with 1 % BSA in PBS. Staining was analyzed using a FACS Calibur instrument (BD PharMingen).
Dong S, & Chen J. (2015). SUMOylation of sPRDM16 promotes the progression of acute myeloid leukemia. BMC Cancer, 15, 893.
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3

Quantifying Monocyte Surface Markers

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Cells were isolated and blocked with 1% BSA in PBS for 10 min at room temperature. Fixed and blocked cells were incubated with APC-conjugated anti-CD11b monoclonal antibody (BD PharMingen) for 30 min on ice, and cells were washed three times with 1% BSA in PBS. Staining was analyzed using a FACS Calibur instrument (BD PharMingen).
Chen X., Qin Y., Zhang Z., Xing Z., Wang Q., Lu W., Yuan H., Du C., Yang X., Shen Y., Zhao B., Shao H., Wang X., Wu H, & Qi Y. (2021). Hyper-SUMOylation of ERG Is Essential for the Progression of Acute Myeloid Leukemia. Frontiers in Molecular Biosciences, 8, 652284.
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4

Neutrophil Isolation and NET Formation

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The bone marrow of the femur and tibia were collected from WT mice as previously described by Cools-Lartigue et al (15 ). Neutrophils were sorted with a BD-Aria-Plus high-speed sorter using APC conjugated anti-CD11b monoclonal antibody and APC-Cy7 conjugated anti-Lys6G monoclonal antibody (both BD Pharmingen). 2×106 neutrophils were plated onto gelatin coated 6cm dishes and onto gelatin coated glass cover slips, and allowed to attach to the plate during a 1 hour incubation period at 37°C. The FFA palmitic (C16:0), linoleic (C18:2), and oleic acid (C18:1) (all from Nu-Check Prep) were dissolved in 25% FFA-free BSA and were added in a final concentration of 50uM to stimulate the neutrophils. LPS at 100nM and H2O2 (both Sigma) were used as positive controls, whereas 25% BSA without FFA served as negative control. After 4 hours of stimulation at 37°C, the supernatants were spun and frozen for analysis of MPO-DNA levels. Cover slips were stained with antibodies against histone 2AX (Abcam ab20669, with Alexa Fluor 555 goat anti-rabbit secondary antibody from Life Technologies), actin (Alexa Fluor 488, Invitrogen) and DAPI, and NET formation was visualized with an Olympus Fluoview 1000 microscopic camera.
van der Windt D.J., Sud V., Zhang H., Varley P.R., Goswami J., Yazdani H.O., Tohme S., Loughran P., O’Doherty R.M., Minervini M.I., Huang H., Simmons R.L, & Tsung A. (2018). NEUTROPHIL EXTRACELLULAR TRAPS PROMOTE INFLAMMATION AND DEVELOPMENT OF HEPATOCELLULAR CARCINOMA IN NON-ALCOHOLIC STEATOHEPATITIS. Hepatology (Baltimore, Md.), 68(4), 1347-1360.
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