Regular immunoblotting was performed for protein detection following SDS‐PAGE electrophoresis. The primary antibodies used for western blot analysis include: anti‐FLAG® (F7425) (1:2000), anti‐FLAG® M2 (F1804) (1:4000), anti‐c‐Myc (C3956) (1:2000) from Sigma‐Aldrich (St. Louis, MO); anti‐Myc Tag, clone 4A6 (05‐724) (1:4000) from Millipore Corporation (Billerica, MA); anti‐ CK1α1 [EPR1961(Mendoza et al. 2007 )] (ab108296) (1:2000), anti‐CK1δ [AF12G4] (ab85320) (1:4000), anti‐CK1ε [AF6C1] (ab82426) (1:2000) from abcam® (Cambridge, MA); anti‐HaloTag® (G9211) (1:1000) from Promega Corporation (Madison, WI). Two secondary antibodies were used: ECL anti‐rabbit IgG (NA934V; GE Healthcare; Little Chalfont, U.K.) and anti‐mouse IgG+IgM HRP (ab47827; abcam®; Cambridge, MA).
Anti c myc c3956
Manufactured by Merck Group
Anti‐c‐Myc (C3956) is a laboratory reagent used in research applications. It serves as an antibody that specifically binds to the c‐Myc protein, which is a transcription factor involved in cell growth and proliferation. This reagent can be used in various experimental techniques, such as immunoprecipitation, Western blotting, and immunohistochemistry, to detect and study the c‐Myc protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti flag f7425, by Merck Group (2 mentions)
Anti flag m2 f1804, by Merck Group (1 mentions)
Ab108296, by Abcam (1 mentions)
Ecl anti rabbit igg na934v, by GE Healthcare (1 mentions)
Anti halotag g9211, by Promega (1 mentions)
Fjk 16, by Thermo Fisher Scientific (1 mentions)
Anti phosphoserine threonine tyrosine antibody, by Abcam (1 mentions)
Anti ha h3663, by Merck Group (1 mentions)
Gateway technology, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti c myc c3956
1
Immunoblotting Antibody Detection Protocol
2
Multicolor Flow Cytometry for Immune Cell Profiling
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The following antibodies were purchased from eBioscience and used for FACS assay: anti–mouse CD3e (145-2C11), anti–mouse CD4 (GK1.5), anti–mouse CD8a (53–6.7), anti–mouse CD45R (B220; RA3-6B2), anti–mouse IL-17A (eBio17B7), anti–mouse IFN-γ (XMG1.2), anti–mouse CD11b (M1/70), anti–mouse CD29 (eBioHMb1-1), and anti–mouse CXCR4 (2B11). The following antibodies were purchased from BD: anti–mouse-Vα2 (B20.1), anti–mouse-Vα3.2 (553219), anti–mouse-Vβ6 (553194), anti–mouse-Vβ8.1/8.2 (118405), anti–mouse-Vβ8.3 (118603), anti–mouse-Vβ11 (125907), and anti–mouse-Vβ14 (553258). The following antibodies were purchased from to be added: anti–mouse CCR5 (HM-CCR5; BD), anti–mouse CD44 (IM7; eBioscience), and anti–mouse Foxp3 (FJK-16S; eBioscience). Anti–human CD4 (S5.3; Invitrogen), anti–human DOCK8 (sc-104911; Santa Cruz Biotechnology, Inc.), and anti–human LRCH1 (sc-84195; Santa Cruz Biotechnology, Inc.) were used for FACS or immunoblotting assay. Anti-Phosphoserine/threonine/tyrosine antibody (ab15556; Abcam), anti-FLAG (F3165; Sigma-Aldrich), anti-HA (H3663; Sigma-Aldrich), anti-c-Myc (C3956; Sigma-Aldrich), and anti-Cdc42 (sc-87; Santa Cruz Biotechnology, Inc.) were used for immunoprecipitation or immunoblot assay.
3
Generating Transgenic Arabidopsis Plants for Functional Studies
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Plasmid constructs were generated either via restriction enzyme or Gateway technology (Invitrogen) and were confirmed by DNA sequencing. All the primers with restriction sites used in this study are listed in Supplemental Table S1 . All the constructs used in this study are listed in Supplemental Table S2 . Plasmid constructs were transferred into Agrobacterium tumefaciens strain GV3101 and used to transform plants by the floral-dip method (Clough and Bent, 1998 (link)). 35S:BAF1-FLAG and 35S:BAF1-ΔF-FLAG overexpressing plants were screened on 1/2 LS plates supplemented with 50 mg/L kanamycin and further confirmed by immunoblotting using anti-FLAG (F7425, Sigma–Aldrich) antibodies. 35S:BAF1-MYC and 35S:BAF1-ΔF-MYC overexpressing plants in the background of bes1-D or 35S:BES1-GFP were screened on 1/2 LS plates supplemented with 75 mg/L gentamycin and further confirmed by immunoblotting using anti-c-MYC (C3956, Sigma–Aldrich) antibodies. Homozygous T3 lines of these transgenics were further identified for experimental use in this study. 35S:BAF1-FLAG overexpressing plants in DSK2 RNAi background were screened on 1/2 LS plates supplemented with 50 mg/L kanamycin plus 10 mg/L herbicide Basta and T2 and T3 lines were used.
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