Mouse interferon γ ifn γ

SBOSs were purchased from Baolingbao Biological Co. (Shandong, China). Chicken red blood cells (CRBCs) and lactate dehydrogenase were obtained from Applygen Technologies Inc. (Beijing, China). Methyl thiazolyl tetrazolium (MTT) and sodium dodecyl sulfate (SDS) were purchased from AMRESCO LLC. (Solon, USA). Concanavalin A (ConA) and erythrocyte lysates were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Mouse tumor necrosis factor-α (TNF-α), mouse interleukin-4 (IL-4), and mouse interferon-γ (IFN-γ) were purchased from R&D Systems, Inc. (San Diego, CA, USA). Mouse TNF-α, IL-4, and IFN-γ ELISA kits were purchased from Bogu Biological Technology Co. (Shanghai, China). Biological stains, including Giemsa dye, vice magenta, and malachite green were obtained from Aojia Chemical Co. (Nanjing, China).
Kunming mice (weighing 22.2 ± 0.6 g each) were obtained from Biotechnology Co., Ltd. (Liaoning, China) and maintained in specific pathogen-free conditions. They had free access to water and standard rodent chow and were weighed daily.

All reagents for cell culture were obtained from Lonza. Ficoll-Paque Plus used for peripheral blood mononuclear cell (PBMC) isolation was provided by GE Healthcare. MTT, lipopolysaccharide (LPS), sulphanilic acid, N-(1-naphtyl) ethylenediaminedihydrochloride, 2′7′-dichlorofluorescin diacetate (DCFH-DA) nitroblue tetrazolium, riboflavin, etoposide, Drabkin’s reagent, BRIJ^® L23 solution, bovine hemoglobin, Dulbecco’s PBS (DPBS), N,N,N′,N′-tetrame-thylethylenediamine and phorbol 12-myristate 13-acetate (PMA) were purchased from Sigma-Aldrich. Mouse interferon γ (IFN-γ) was obtained from R&D Systems. Homemade recombinant human tumor necrosis factor (TNF) was purified from the culture media of mouse fibroblasts stably expressing TNF by affinity chromatography on anti-TNF monoclonal antibody conjugated to Sepharose. Biological activity of TNF was standardized using human TNF provided by Sigma-Aldrich. Lack of LPS contamination was verified with E-TOXATE kit (Sigma-Aldrich).