Anti cd20 pe vio770

Manufactured by Miltenyi Biotec

Anti-CD20-PE-Vio770 is a fluorescently labeled antibody that binds to the CD20 antigen. It is designed for use in flow cytometric analysis.

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CD20-PE-Vio770 (2 citations) Anti-CD20 (2 citations)

2 protocols using anti cd20 pe vio770

Flow Cytometric Characterization of nDCs

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Purity and phenotype of nDCs after immunomagnetic isolation were determined by flow cytometry with a FACSVerse® (BD biosciences, San Jose, CA) or MACS Quant® (Miltenyi Biotec). For this purpose, the following primary monoclonal antibodies and the appropriate isotype or fluorescence minus one controls were used: anti-CD1c-Viobright-FITC, anti-BDCA2-PE, anti-CD123-APC, anti-CD20-PE-Vio770, anti-CD45-APC-Vio770, anti-CD14-Viogreen, anti-FcεRI-BioBlue, anti-CD14-FITC, anti-CD15-PE, anti-CD56-APC, anti-CD3-VioBlue, anti-HLA-ABC-APC, anti-HLA-DR/DP/DQ-APC, anti-CCR7-APC, anti-CD80-APC, anti-CD83-APC, and anti-CD86-APC (all Miltenyi Biotec). Details are depicted in Supplementary Table 2. The purity of the nDC product was defined as the percentage of nDCs (sum of CD123⁺BDCA2⁺ pDC plus CD1c⁺CD20^- cDC2) of all viable cells in the nDC product. After 6 h of protamine/mRNA stimulation, cytokine production of nDCs was measured in the supernatant by cytometric bead array according to the manufacturer’s instruction (Miltenyi Biotec).

Bol K.F., Schreibelt G., Bloemendal M., van Willigen W.W., Hins-de Bree S., de Goede A.L., de Boer A.J., Bos K.J., Duiveman-de Boer T., Olde Nordkamp M.A., van Oorschot T.G., Popelier C.J., Pots J.M., Scharenborg N.M., van de Rakt M.W., de Ruiter V., van Meeteren W.S., van Rossum M.M., Croockewit S.J., Koeneman B.J., Creemers J.H., Wortel I.M., Angerer C., Brüning M., Petry K., Dzionek A., van der Veldt A.A., van Grünhagen D.J., Werner J.E., Bonenkamp J.J., Haanen J.B., Boers-Sonderen M.J., Koornstra R.H., Boomsma M.F., Aarntzen E.H., Gotthardt M., Nagarajah J., de Witte T.J., Figdor C.G., de Wilt J.H., Textor J., de Groot J.W., Gerritsen W.R, & de Vries I.J. (2024). Adjuvant dendritic cell therapy in stage IIIB/C melanoma: the MIND-DC randomized phase III trial. Nature Communications, 15, 1632.

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Phenotypic Characterization of Isolated mDCs and pDCs

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Purity and phenotype of mDCs and pDCs after CliniMACS isolation were determined by flow cytometry with a FACSVerse (BD Biosciences, San Jose, CA, USA) or MACS Quant (Miltenyi Biotec). The following primary monoclonal antibodies and the appropriate isotype or fluorescence minus one controls were used: anti-CD1c-Viobright FITC, anti-BDCA-2-PE, anti-CD20-PE-Vio770, anti-CD123-APC, anti-CD45-APC-Vio770, anti-CD14-VioGreen, anti-FcεRI-VioBlue, anti-CD14-FITC, anti-CD15-PE, anti-CD56-APC, anti-CD3-BioBlue, anti-HLA-ABC-APC, anti-HLA-DR,DP,DQ-APC, anti-CCR7-APC, anti-CD80-APC, anti-CD83-APC and anti-CD86-APC (all Miltenyi Biotec).

Westdorp H., Creemers J.H., van Oort I.M., Schreibelt G., Gorris M.A., Mehra N., Simons M., de Goede A.L., van Rossum M.M., Croockewit A.J., Figdor C.G., Witjes J.A., Aarntzen E.H., Mus R.D., Brüning M., Petry K., Gotthardt M., Barentsz J.O., de Vries I.J, & Gerritsen W.R. (2019). Blood-derived dendritic cell vaccinations induce immune responses that correlate with clinical outcome in patients with chemo-naive castration-resistant prostate cancer. Journal for Immunotherapy of Cancer, 7, 302.

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