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1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc

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1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) is a chemical compound commonly used in various laboratory applications. It is a water-soluble carbodiimide that facilitates the formation of amide bonds between carboxyl groups and primary amines. EDC is a versatile reagent that can be used in a wide range of chemical reactions and biological applications.

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Lab products found in correlation

N hydroxysuccinimide nhs, by Merck Group (29 mentions) N hydroxysuccinimide, by Merck Group (17 mentions) Bovine serum albumin (bsa), by Merck Group (9 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (8 mentions) Sodium hydroxide (naoh), by Merck Group (8 mentions) N hydroxysulfosuccinimide nhs, by Merck Group (7 mentions) N hydroxysulfosuccinimide sodium salt sulfo nhs, by Merck Group (6 mentions) Phosphate buffered saline (pbs), by Merck Group (6 mentions) Ethanol, by Merck Group (5 mentions) 3 mercaptopropionic acid, by Merck Group (5 mentions) 2 n morpholino ethanesulfonic acid mes, by Merck Group (5 mentions) Tween 20, by Merck Group (5 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (4 mentions) Streptomycin, by Thermo Fisher Scientific (4 mentions) 11 mercaptoundecanoic acid, by Merck Group (4 mentions) Hydrochloric acid (hcl), by Merck Group (3 mentions) L glutamine, by Thermo Fisher Scientific (3 mentions) Penicillin, by Thermo Fisher Scientific (3 mentions) Potassium ferricyanide, by Merck Group (3 mentions) Chloroform, by Merck Group (3 mentions)

Spelling variants of this product

1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (189 citations) 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (149 citations) 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (99 citations) Ethyl(dimethylaminopropyl) carbodiimide (EDC) (6 citations) 1-ethyl-3-(dimethylaminopropyl) carbodiimide (EDC), (5 citations) 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride (4 citations) 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (3 citations) Ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) (3 citations) 1-ethyl-3-(dimethylaminopropyl) carbodiimide (3 citations) Ethyl carbodiimide hydrochloride (EDC) (2 citations)

127 protocols using 1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc

1

Synthesis and Functionalization of Magnetic Nanoparticles

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Iron acetylacetonate (Fe (acac)3), 1,2-hexadecanediol, benzyl ether, oleyamine (OLA), oleic acid (OA), sodium dodecyl sulfate (SDS), dopamine hydrochloride (DP), 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) were purchased from Millipore-Sigma (Darmstadt, Germany). Dulbecco’s Modified Eagle’s Medium (DMEM) with high glucose and fetal bovine serum (FBS) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). NH2-PEG-COOH (molecular weight =2,000 Da) was purchased from Seebio Biological (Shanghai, China). DOX hydrochloride was purchased from Sangon Ltd. (Shanghai, China). Anti-EGFR antibody was obtained from Ruiying Biological (Suzhou, China). Other reagents (analytical grade) were purchased from Beijing Chemical Reagents Company (Beijing, China) unless otherwise stated.
Mu X., Zhang F., Kong C., Zhang H., Zhang W., Ge R., Liu Y, & Jiang J. (2017). EGFR-targeted delivery of DOX-loaded Fe3O4@ polydopamine multifunctional nanocomposites for MRI and antitumor chemo-photothermal therapy. International Journal of Nanomedicine, 12, 2899-2911.
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2

Carboxyl Group Activation with EDC/NHS

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Carboxyl groups (-COOH), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, MW = 191.70 g/mol; MilliporeSigma), and N-hydroxysuccinimide (NHS, purity >98%, MW = 115.09 g/mol; MilliporeSigma) solutions (0.01 M) were prepared and mixed at a 1:1 ratio in 0.1 M 2-(N-morpholino)ethanesulfonic acid (MES) buffer (MES sodium salt, MW = 217.2 g/mol; MilliporeSigma).
Ho K.N., Chen L.W., Kuo T.F., Chen K.S., Lee S.Y, & Wang S.F. (2022). Surface modification of zirconia ceramics through cold plasma treatment and the graft polymerization of biomolecules. Journal of Dental Sciences, 18(1), 73-80.
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3

Synthesis and Characterization of Functionalized Nanoparticles

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Silver nitrate (AgNO3), sodium borohydride (NaBH4), L-ascorbic acid, iron (III) chloride hexahydrate (FeCl3.6H2O), iron (II) chloride tetrahydrate (FeCl2.4H2O), chromium trioxide (CrO3), polyethylene glycol (PEG, Mw~6000, Mw~10000 kDa), methoxy polyethylene glycol (m-PEG, Mw~6000 kDa), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), ammonia (NH3, 25%), hydrochloric acid (HCl, 37%), sulfuric acid (H2SO4, 96%), acetone and isopropanol were purchased from Merck. N-hydroxysuccinimide (NHS), cystamine dihydrochloride, penicillin-streptomycin and 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) were obtained from Sigma-Aldrich. Cetyl trimethylammonium bromide (CTAB) and methanol were purchased from Chemlab. Hydrogen tetrachloroaurate (HAuCl4.3H2O) was obtained from Alfa Aesar. Fetal bovin serum (FBS) and RPMI 1640 and high glucose DMED cell culture mediums were purchased from Gibco. All chemicals were of analytical grade and used as received without further purifications. For the preparation of all samples, deionized (DI) water with resistivity of 18.2 MΩ was used.
Khafaji M., Vossoughi M., Hormozi-Nezhad M.R., Dinarvand R., Börrnert F, & Irajizad A. (2016). A new bifunctional hybrid nanostructure as an active platform for photothermal therapy and MR imaging. Scientific Reports, 6, 27847.
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4

Versatile SARS-CoV-2 Antigen Detection Aptasensor

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SARS-CoV-2 alpha, beta,
delta, gamma, and omicron antigens were obtained from BiosPacific
(BiosPacific, Emeryville, CA, USA) and stored at −80 °C.
Amino-functionalized COVID-19 aptamers were synthesized by Metabion
International (Planegg, Germany), and stored at −20 °C.
50 nm blue, red, orange, and yellow nanobeads were obtained from Polysciences
Inc. (Taipei, Taiwan) and stored at 4 °C. ACE2, phosphate buffer
saline (PBS), 1-ethyl-3-(3-(dimethylamino)propyl) carbodiimide hydrochloride
(EDC), and N-hydroxysuccinimide (NHS) were purchased
from Merck Life Science UK Ltd (Dorset, UK). Finally, the cotton swabs
were purchased from a local pharmacy in Riyadh, Saudi Arabia.
Aloraij Y.M., Suaifan G.A., Shibl A., Al-Kattan K, & Zourob M.M. (2023). Development of Rapid Aptamer-Based Screening Assay for the Detection of Covid-19 Variants. ACS Omega, 8(36), 32877-32883.
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5

Constructing an In Vitro BBB Model

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Lactoferrin (Lf), Triton X-100, Tris (2,2-bipyridyl) dichlororuthenium(II) hexahydrate (Rubpy), n-hexanol, tetraethyl orthosilicate (TEOS), 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) were ordered from Sigma-Aldrich (MA, USA). Bis-carboxy poly(ethylene glycol) (MW~10 kDa) was purchased from Nanocs (Tokyo, Japan). Collagen G, medium 199, DMEM medium, bovine calf serum, L-glutamine, gentamycin, penicillin, fetal bovine serum (FBS) and streptomycin were ordered from Gibco (MA, USA) to construct our in vitro BBB model.
Song Y., Du D., Li L., Xu J., Dutta P, & Lin Y. (2017). In Vitro Study of Receptor-Mediated Silica Nanoparticles Delivery across Blood-Brain Barrier. ACS applied materials & interfaces, 9(24), 20410-20416.
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6

Nanocomposite Scaffold Fabrication

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Chitosan (Chi, 95% degree of deacetylation, 150–300 kDa; Heppe Medical Chitosan GmbH, Germany) was used as received; three different types of nanocellulose suspensions (BioPlus® hydrophilic cellulose, American Process, Atlanta, GA): nanofibrils (CNF, 5–20 nm diameter, 500 nm length, 3% w/w), nanocrystals (CNC, 4–5 nm diameter, 50–500 nm length, 6% w/w), and nanoblend (CNB: CNF and CNC mixed in 2:3 ratio, 3% w/w) were used as received76 (link); and sodium hydroxide (anhydrous, reagent grade, Sigma Aldrich, St. Louis, MO, USA), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Sigma Aldrich, St. Louis, MO, USA), acetic acid (Glacial, ACS grade, EMD Millipore, Burlington, MA, USA), phosphate buffered saline (PBS, biotechnology grade; VWR, Radnor, PA, USA), and 200 proof ethanol (VWR, Radnor, PA, USA) were used as received.
Yin K., Divakar P, & Wegst U.G. (2019). Plant-derived Nanocellulose as Structural and Mechanical Reinforcement of Freeze-Cast Chitosan Scaffolds for Biomedical Applications. Biomacromolecules, 20(10), 3733-3745.
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7

Lymphatic-Targeted PLGA Nanoparticles for Cancer

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PLGA (lactide: glycolide = 50:50, MW = 12,000 Da) was purchased from Jinan Daigang Biological Material Limited Company (Shandong, China). PFH was purchased from J&K Scientific Ltd. (Beijing, China). DOX was obtained from Beijing Yihe Biological Co. Ltd. (Beijing, China). GSN monoclonal antibodies, poly(vinyl alcohol) (PVA, 99% MW`=30,000–70,000), 2-(N-morpholino)ethanesulfonic acid (MES), 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), 1,1-dioctadecyl-3,3,3,3-tetrame-thylindocarbocyanine perchlorate (DiI), 4,6-diamino-2-phenylindole (DAPI), and coumarin-6 were purchased from Sigma–Aldrich (St. Louis, MO, USA). The use of a mouse ascitic hepatocarcinoma cell line with high lymphatic metastasis (Hca-F) was approved by the ethics committee of the Affiliated Lianyungang Hospital of Xuzhou Medical University (LW-20180611012). All chemicals used in this work were analytical grade and used without further processing.
Qin H., Teng R., Liu Y., Li J, & Yu M. (2022). Drug Release from Gelsolin-Targeted Phase-Transition Nanoparticles Triggered by Low-Intensity Focused Ultrasound. International Journal of Nanomedicine, 17, 61-71.
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8

VEGF-Based Protein Conjugation Protocol

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Human recombinant VEGF165, anti-VEGF165 antibody, N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), dimethylformamide (DMF) and ethanol (95%) were purchased from Sigma-Aldrich, India. Just to highlight that anti-VEGF antibody and VEGF165 were stored at 4 °C and −20 °C, respectively, before use. Freshly aqueous solutions of EDC and NHS were prepared before using. All solutions were prepared using deionized water (DI) from a Millipore system. Cobaltous chloride, potassium ferricyanide, 1,3,5-benzenetricarboxylic acid (BTC) and GO were purchased from Merck. Sodium dihydrogen orthophosphate dehydrate (Merck) and sodium phosphate dibasic dehydrate (Sigma-Aldrich) were used to prepare a 0.1 M phosphate buffer of different pH to carry out pH study. All chemicals were of analytical grade and used without any further purification.
Singh S., Numan A., Zhan Y., Singh V., Alam A., Van Hung T, & Nam N.D. (2020). Low-potential immunosensor-based detection of the vascular growth factor 165 (VEGF165) using the nanocomposite platform of cobalt metal–organic framework. RSC Advances, 10(46), 27288-27296.
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9

Porous Hydrogel Scaffold Fabrication

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Gelatin type A from porcine
skin (bloom 300), GA (25% solution in water), glycine, 1-ethyl-3-(3-dimethyl-aminopropyl)carbodiimidehydrochloride
(EDC), diethylpyrocarbonate, glycyrrhetinic acid, and collagenase
from Clostridium histolyticum in addition
to the chemicals that were used in the cytotoxicity studies: dimethyl
sulfoxide (DMSO), MTT, and trypan blue dye were purchased from Sigma,
St. Louis, Mo., USA. Acetone was purchased from Labscan, Dublin, Ireland.
Allicin was purchased from Jiangsu chiataiqingjiang Pharmaceutical
Co., Ltd., China. Spectra/Por dialysis membrane, 12 000–14 000
molecular weight cutoff, was purchased from Spectrum Laboratories
Inc., Rancho Dominguez, Canada.
Ossama M., Hathout R.M., Attia D.A, & Mortada N.D. (2019). Enhanced Allicin Cytotoxicity on HEPG-2 Cells Using Glycyrrhetinic Acid Surface-Decorated Gelatin Nanoparticles. ACS Omega, 4(6), 11293-11300.
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10

EDA core PAMAM dendrimer functionalization

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EDA core PAMAM dendrimer generation 4 (technical grade) was purchased from Dendritech (Midland, MI). Triglycine, N-hydroxysuccinimide (NHS), and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) were purchased from Sigma-Aldrich (St. Louis, MO). Recombinant human EGF was purchased from Austral Biologicals (San Ramon, CA). Antibodies that recognize EPS8 (clone 15) and actin (sc-1616) were purchased from BD Biosciences (Carlsbad, CA) and Santa Cruz Biotechnologies Inc. (Santa Cruz, CA), respectively. Horseradish peroxidase-conjugated secondary antibodies were obtained from MP Biomedicals (Aurora, OH). TransIT keratinocyte transfection reagent (simply referred to as TransIT) was obtained from Mirus Bio (Madison, WI). EPS8 siRNA was purchased from Qiagen (Valencia, CA). EPS8 shRNA was prepared as described in our previous publication (Wang et al., 2010 (link)).
Di Marco S., Hel Z., Lachance C., Furneaux H, & Radzioch D. (2001). Polymorphism in the 3′-untranslated region of TNFα mRNA impairs binding of the post-transcriptional regulatory protein HuR to TNFα mRNA. Nucleic Acids Research, 29(4), 863-871.
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