Penicillin streptomycin

Manufactured by Bio Basic

Sourced in United States

Penicillin/streptomycin is a sterile solution containing the antibiotics penicillin and streptomycin. It is commonly used in cell culture applications to prevent bacterial contamination.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions) Waymouth s media, by Thermo Fisher Scientific (2 mentions) M199 medium, by Thermo Fisher Scientific (2 mentions) Tryptose phosphate, by Merck Group (2 mentions) Penicillin streptomycin, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Merck Group (1 mentions) 3 isobutyl 1 methyl xanthine (ibmx), by Merck Group (1 mentions) Fetal bovine serum (fbs), by Wisent (1 mentions) Glutamine, by Wisent (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium dmem f12, by Thermo Fisher Scientific (1 mentions) Collagenase dispase, by Roche (1 mentions) Forskolin, by Merck Group (1 mentions) Bovine pituitary extract, by Merck Group (1 mentions) 24 well plate, by SPL Life Sciences (1 mentions) 4 hne, by Merck Group (1 mentions) Dmem f12 media, by Thermo Fisher Scientific (1 mentions) 12 well cell culture plate, by SPL Life Sciences (1 mentions)

Spelling variants of this product

Streptomycin (21 citations) Penicillin (12 citations)

10 protocols using penicillin streptomycin

Adipogenic Differentiation and Mouse Embryonic Stem Cell Culture

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Pre-adipocyte 3T3-L1 cells were grown in DMEM medium supplemented with 10% FBS (Gibco), 2 mM glutamine (Wisent) and 1% Penicillin/Streptomycin (Biobasic). For adipogenic differentiation of 3T3L1, the cells were plated at confluency and media was changed to induction media containing 10% FBS, 1% Penicillin/Streptomycin, 1 μM Dexamethasone, 1 μg/ml Insulin and 500 μM IBMX (Sigma). Two days post-induction, the medium was changed to maintenance media containing 10% FBS (Gibco), 1% Penicillin/Streptomycin (Biobasic), 1 μg/ml Insulin. After 3 days post-induction, 10,000 cells were plated on homemade chambers for sorting.
Mouse Embryonic Stem cell (mES) culture mES cells were grown in DMEM medium supplemented with 15% FBS (embryonic stem cell qualified, Wisent), 1 X non-essential amino acids (Sigma), 100 μM 2-Mercaptoethanol (Gibco), 1000 Units/mL Leukemia inhibitory factor (LIF, Stemcell), 2 mM glutamine (Wisent) and 1% Penicillin/Streptomycin (Biobasic) on 0.1% porcine gelatin-coated plastic dishes (Sigma). About 10,000 cells were plated for sorting as above.

Binan L., Bélanger F., Uriarte M., Lemay J.F., Pelletier De Koninck J.C., Roy J., Affar E.B., Drobetsky E., Wurtele H, & Costantino S. (2019). Opto-magnetic capture of individual cells based on visual phenotypes. eLife, 8, e45239.

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Orexin Signaling in Hepatocytes

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LMH cells (ATCC CRL-2117) or spontaneously immortalized chicken embryonic hepatocytes (siCEH^{22 (link)}) were grown on 0.1% gelatin-coated culture dishes in Waymouth’s media (Life Technologies, Carlsbad, CA), supplemented with 10% FBS (Life Technologies, Carlsbad, CA), and 1% penicillin–streptomycin (BioBasic, Amherst, NY) at 37 °C, in a humidified atmosphere of 5% CO₂ and 95% air. At ~ 80% confluence, cells were synchronized with serum-free media overnight and treated with different doses (0, 10, and 100 nM) of recombinant human ORX-A or ORX-B (Alpha Diagnostic International, San Antonio, TX) for 24 h. The dose of orexins was selected based on pilot and previous studies^{16 (link),20 (link),23 (link),24 (link)}. 30 min before orexin treatments, LMH cells were incubated with brefeldin A (0.3 µg/mL) for 24 h and lysates and medium were subjected to immunoblot analyses. All culture experiments were performed with cells from passages 10–15 and repeated at least twice.

Greene E.S., Zampiga M., Sirri F., Ohkubo T, & Dridi S. (2020). Orexin system is expressed in avian liver and regulates hepatic lipogenesis via ERK1/2 activation. Scientific Reports, 10, 19191.

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Isolation and Culture of Rat Mesenchymal Stem Cells

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Mesenchymal stem cells were isolated from bone marrow of Wistar rats aged between 5 and 6 weeks as previously described (Soleimani and Nadri, 2008 (link)). r-BMSCs, human embryonic kidney cells 293 (HEK-293T), and glioblastoma cell line (U87) were cultured in Dulbecco modified Eagle medium (Gibco, Grand Island, NY, United States) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin/streptomycin (Bio Basic, Markham, Ontario, Canada) in a 37°C with 5% CO₂ incubator.

Jahangard Y., Monfared H., Moradi A., Zare M., Mirnajafi-Zadeh J, & Mowla S.J. (2020). Therapeutic Effects of Transplanted Exosomes Containing miR-29b to a Rat Model of Alzheimer’s Disease. Frontiers in Neuroscience, 14, 564.

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Serum Starvation of QM7 Cells

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QM7 cells were grown in M199 medium (Life Technologies, Grand Island, NY) with 10% fetal bovine serum (Life Technologies), 10% tryptose phosphate (Sigma-Aldrich, St. Louis, MO), and 1% penicillin-streptomycin (Biobasic, Amherst, NY) at 37 °C under a humidified atmosphere of 5% CO2 and 95% air. At 80–90% confluence, cells were synchronized overnight in serum-free medium.

Bottje W., Kong B.W., Reverter A., Waardenberg A.J., Lassiter K, & Hudson N.J. (2017). Progesterone signalling in broiler skeletal muscle is associated with divergent feed efficiency. BMC Systems Biology, 11, 29.

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Enrichment and Culture of Schwann Cells from Rat Sciatic Nerve

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Schwann cells (SCs) were enriched from pre-degenerated sciatic nerves of adult Sprague-Dawley rats as described in a previous study by our group (24 (link)). The protocol is based on SC proliferation after Wallerian degeneration, combined use of collagenase/dispase and mitogen in culture medium. In brief, the sciatic nerves were ligated to allow the nerves to pre-degenerate. The pre-degenerated nerves ~20 mm in length were cut into 1-mm pieces. The minced nerves were enzymatically dissociated using an enzyme mixture of 0.05% collagenase/dispase (Roche, Basel, Switzerland) in Dulbecco's modified Eagle's medium (DMEM)/F12 (Gibco-BRL, Invitrogen Life Technologies, Carlsbad, CA, USA) for 5.5 h at 37°C. Following dissociation, the cell suspension was centrifuged at 200 x g for 10 min. The dissociated SCs were re-suspended and cultured in DMEM/F12 supplemented with 10% fetal bovine serum (FBS; Kibbutz BeitHaemek, Israel), 1% penicillin/streptomycin (BioBasic), 2 µM forskolin (0.8 µg/l; Sigma-Aldrich, St Louis, MO, USA) and 20 µg/l bovine pituitary extract (Sigma-Aldrich). After the cultures reached confluence, they were used for graft procedures.

ZHOU L.N., CUI X.J., SU K.X., WANG X.H, & GUO J.H. (2015). Beneficial reciprocal effects of bone marrow stromal cells and Schwann cells from adult rats in a dynamic co-culture system in vitro without intercellular contact. Molecular Medicine Reports, 12(4), 4931-4938.

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Immortalized Chicken Liver Cells Stress Response

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Spontaneous immortalized chicken embryo liver cells (sim-CEL, Piekarski et al., 2014 (link)) were grown in Waymouth's media (Life Technology, Waltham, MA) supplemented with 10% fetal bovine serum (Life Technologies, Waltham, MA), and 1% penicillin/streptomycin (Biobasic, Amherst, NY) at 37°C under a humidified atmosphere of 5% CO₂ and 95% air. At 80–90% confluency, cells were exposed to two environmental conditions (45°C vs. 37°C) and two treatments (50 μM quercetin, QCT vs. control) for 4 h. The dose of QCT was selected based on pilot and previous studies (Dok-Go et al., 2003 (link); Martirosyan et al., 2016 (link); Wang et al., 2017 (link))

Flees J., Rajaei-Sharifabadi H., Greene E., Beer L., Hargis B.M., Ellestad L., Porter T., Donoghue A., Bottje W.G, & Dridi S. (2017). Effect of Morinda citrifolia (Noni)-Enriched Diet on Hepatic Heat Shock Protein and Lipid Metabolism-Related Genes in Heat Stressed Broiler Chickens. Frontiers in Physiology, 8, 919.

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Dexamethasone Regulation of Target Genes

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A549 and HEK293T cell lines were obtained from Iranian biological resource center (IBRC, Iran). NT2 cells were gifted by Dr. Andrews’s lab. A549 and NT2 Cells were cultured in high glucose Dulbecco’s Modified Eagle Medium (DMEM) and HEK293T cells were grown in DMED/F12 (Thermo Fisher Scientific, USA), supplied with 10% FBS (Thermo Fisher Scientific, USA), and 1% penicillin/streptomycin (Bio Basic, Canada) and incubated in 37 °C with 5% humified CO2. hESC-RH5 was obtained from Royan Institute for Stem Cell Biology and Technology (RI-SCBT, Iran) and was cultivated as described previously⁴⁴.
For investigating the effects of dexamethasone treatment on the expression of targeted genes, cells were seeded in 24-well plates (SPL Life Sciences, South Korea). After complete adhesion, cells were treated with the EC50 concentration of dexamethasone and were lysed for RNA extraction at different time points.

Mirzadeh Azad F., Malakootian M, & Mowla S.J. (2019). lncRNA PSORS1C3 is regulated by glucocorticoids and fine-tunes OCT4 expression in non-pluripotent cells. Scientific Reports, 9, 8370.

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Quail Muscle Cell Stress Responses

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Quail muscle (QM7, Antin and Ordahl, 1991 (link)) cell lines were purchased from American Type Culture Collection (ATCC CRL-1962, Manassas, VA) and were grown in M199 medium (Life Technologies, Grand Island, NY) complemented with 10% FBS (Life Technologies), 10% tryptose phosphate (Sigma-Aldrich, St. Louis, MO), and 1% penicillin-streptomycin (Biobasic, Amherst, NY) at 37°C under a humidified atmosphere of 5% CO₂ and 95% air. The medium was changed every 48 h and cells were subjected, during their exponential phase of growth, to the following treatments:

Acute heat stress exposure (HS): QM7 cells were exposed to HS (45°C) for 0.5, 1, 2, or 4 h. The control cells were maintained at 37°C.

Hydrogen peroxide (H₂O₂) treatment: Cells were treated with 10, 50, 100, or 200 μM of H₂O₂ (Sigma-Aldrich, St. Louis, MO) for 3 h. Untreated cells were used as control.

4-Hydroxynonenal (4-HNE) treatment: Cells were treated with 10, 20, or 30 μM of 4-HNE (Sigma-Aldrich, St. Louis, MO) for 24 h. Untreated cells were used as control.

The dose and time of the above mentioned treatments were chosen based on pilot and previous experiments (Piekarski et al., 2014 (link)).

Nguyen P.H., Greene E., Kong B.W., Bottje W., Anthony N, & Dridi S. (2017). Acute Heat Stress Alters the Expression of Orexin System in Quail Muscle. Frontiers in Physiology, 8, 1079.

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Stable cell lines for miR-21 study

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HEK-293T cells was obtained from the Iranian biological resource center and cultivated in DMEM-F12 media (Gibco, USA), containing 10% fetal bovine serum (FBS, Gibco, USA) and 1% penicillin-streptomycin (Bio Basic, Canada) and seeded in 12-well cell culture plates (SPL Life Science, South Korea). Stable cell line colonies expressing pri-miR-21 and miR-21-sponge, as well as their mock vectors were generated by transfecting the cells at a confluency of 70%, using lipofectamin 3000 (Invitrogen, USA). Stable cell line colonies were produced via antibiotic selection of 4 μg/ml Puromycin (Sigma, Germany) and 2 μg/ml Zeocin (Invitrogen, USA), then the antibiotic concentrations were reduced slowly.

Monfared H., Jahangard Y., Nikkhah M., Mirnajafi-Zadeh J, & Mowla S.J. (2019). Potential Therapeutic Effects of Exosomes Packed With a miR-21-Sponge Construct in a Rat Model of Glioblastoma. Frontiers in Oncology, 9, 782.

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Leptin Signaling Modulation in Cell Lines

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Avian myogenic QM7 (Antin and Ordahl, 1991 (link)), hepatocyte Sim-CEL (Piekarski et al., 2014b (link)), and Chinese hamster ovary CHO-K1 cell lines were grown in their appropriate media complemented with 10% FBS (Life Technologies, Waltham, MA) and 1% penicillin-streptomycin (Biobasic, Amherst, NY) at 37°C under a humidified atmosphere of 5% CO₂ and 95% air, as previously described (Adachi et al., 2008 (link); Piekarski et al., 2014b (link); Lassiter et al., 2015 (link)). At 80–90% confluence, cells were synchronized overnight in serum-free media and treated with leptin alone or in combination with AMPK inhibitors as described below.

Piekarski A., Nagarajan G., Ishola P., Flees J., Greene E.S., Kuenzel W.J., Ohkubo T., Maier H., Bottje W.G., Cline M.A, & Dridi S. (2018). AMP-Activated Protein Kinase Mediates the Effect of Leptin on Avian Autophagy in a Tissue-Specific Manner. Frontiers in Physiology, 9, 541.

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