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107 protocols using cr 300

1

Colorimetric Analysis of Cooked Beef Meatballs

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A colorimeter (CR 300, Minolta, Tokyo, Japan) equipped with a measuring head (CR-300) was used at four different random points on the exterior surface to measure the color qualities of cooked beef meatballs. The color values such as lightness (L*), redness (a*), yellowness (b*), chroma (C*), and hue angle () values were evaluated in this study. The colorimeter was calibrated using the Standard white plate (Y = 89.2; x = 0.921; y = 0.783) under CIE (Commission international de l’eclairage) illumination D65 and d/10° illumination at room temperature before starting the colorimetry performance of the meatballs. The chroma and hue angle values were calculated applying the equations as follows: Chroma=a2+b2
Hue angle=tan1(ba)
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2

Analyzing Fruit Peel Colour with CIE

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Colour was measured using a colorimeter (model CR-300, Minolta, Osaka, Japan). Four readings of the peel were made in four equatorial and equidistant zones of all the fruits. Measurements were made at constant room temperature (22 ± 2 °C). Colour was evaluated according to the Commission Internationale de l’Éclairage (CIE) and expressed as L*, a* and b* colour values [12 (link)]. The coordinates L*, a* and b* indicate the lightness of the colour (L* = 0 and L* = 100 represent the colour white and black, respectively), its position between green and red (the negative and positive values of a* indicate green and red, respectively) and its position between blue and yellow (negative and positive values of b* indicate blue and yellow, respectively) [13 (link)]. The target colour (C* = (a* 2 + b* 2) 1/2), the hue angle (H⁰ = arctan (b*/a*), and colour index (CI = a* × 1000/L* × b*) were also calculated.
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3

Instrumental Color Analysis of Cooked Meat

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The instrumental color analysis was conducted as described by the International Commission on Illumination (CIE) [21 ]. Before color measurement, a cooked sample for each treatment was placed in the flat surface and left at room temperature for 10 min. Meat color was assessed using a colorimeter (CR-300; Minolta Co., Osaka, Japan) to determine the lightness (L*), redness (a*), and yellowness (b*) of the cut surface of the sample. Prior to the measurement, the instrument was calibrated using a standard white plate (Y = 93.60, x = 0.3134, y = 0.3194). For every sample, five measurements were obtained.
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4

Colorimetric Analysis of Raw Meatball Color

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A colorimeter (CR-300, Konica Minolta, Tokyo, Japan) was used to measure the color characteristics of the exterior surface of the raw meatballs. The color values, i.e., a* (redness), b* (yellowness), and L* (lightness) were assessed in triplicate (Mashau et al., 2021 (link)). Metmyoglobin percentage was calculated using Equation (2) by measuring the absorbance of the meat supernatant at 525, 545, 565, and 575 nm, as described by Ouerfelli et al. (2019) (link). Metmyoglobin%=-2.51×A572A525+0.777×A565A525+0.8×A545A525+1.098100
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5

Colorimetric Analysis of Cooked Meat Batters

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The lightness (L*), redness (a*), and yellowness (b*) of the cooked meat batters were measured using a spectrophotometer (CR-300; Minolta Inc., Japan). Measurements were taken at 3 different locations per sample, perpendicular to the surface of the cooked meat batter, with illumination area 30 mm in diameter.
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6

Postmortem Meat Quality Analysis

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The pH of the IF, LT, and SS muscles was measured at 48 h postmortem using a pH meter with a puncture electrode (Model SG2 - ELK Seven Go, Mettler-Toledo International Inc., Giessen, Germany).
The Cielab L* (lightness), a* (redness), and b* (yellowness) values of each muscle sample were measured using a chromameter (CR-300, Konica Minolta, Osaka, Japan) with an illuminant D65 light source. Three measurements were taken from each sample and the average was recorded.
For cooking loss, the samples were weighed, vacuum-sealed using plastic bags and then cooked in a constant 80°C water bath for 30 min or until core temperature of meat sample reached 70°C. The cooked samples were cooled down to room temperature before weighing. Cooking loss of meat sample was expressed as percent weight loss over the initial weight before cooking. Eight 1 cm×2 cm×1 cm slices were removed parallel to the fiber orientation through the thickest portion of the cooked muscle.
Warner-Bratzler shear force was determined by using a universal testing machine (model 2519-104, Instron, Norwood, Massachusetts, USA) equipped with a Warner-Bratzler shear head operating at a crosshead speed of 50 mm/min.
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7

Pork Quality Evaluation Protocol

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At the end of the experiment, 4 pigs from each treatment group were selected and slaughtered for the carcass analysis. Pork samples were collected from nearby the 10th rib on the right side of the carcass. Because of the chilling procedure, 30 minutes after slaughter was regarded as the initial time. The pH, meat, and back fat color of the longissimus muscle (LM) were measured at 24 hours after slaughter. The pH was measured using a pH meter (Model 720, Thermo Orion, Fullerton, CA, USA). Pork and back fat color were determined by Commission Internationale de l’Eclairage (CIE) color L*, a*, and b* values using a CR-300 (Minolta Camera Co., Osaka, Japan). Chemical analysis of the pork samples was conducted using the method of AOAC [10 ].
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8

Liver Color Quantification in Mice

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Changes in the color of the liver were measured using a colorimeter (CR-300, Minolta, Japan). L (0 is black and 100 is white); a (positive and negative values are shown in red and green, respectively); b (positive and negative values are shown in yellow and blue, respectively). Specifically, the liver of the mouse was removed and placed in a petri dish, and the left lobe of the liver was pointed at the light-collecting hole of the colorimeter for measurement.
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9

Measuring Color Changes in Fresh-Cut Cucumbers

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Surface color of fresh‐cut cucumber was measured with a chroma meter (CR‐300, Minolta Co., Osaka, Japan) using L* (lightness), a* (red‐green), and b* (yellow‐blue) values. The color was expressed as yellowing index (Kochhar & Kumar, 2015) of peel and whiteness index (Amanatidou, Slump, Gorris, & Smid, 2000) of fresh‐cut surface. The value of ΔE defines the color difference and is calculated by follows:
Yellowing index (YI)=142.48×bL
Whiteness Index (WI)=100[(100L)2+(a2+b2)]1/2
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10

Colorimetric Analysis of Powdered Samples

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The color of powdered samples was determined using CIELAB (CIE) color scales. L* (whiteness/darkness), a* (redness/greenness), and b* (yellowness/blueness) values were measured using a colormeter (CR-300, Konica Minolta Inc., Tokyo, Japan). Five measurements were averaged, and the total color difference (ΔE) estimated using the following equation reported by [25 (link)].
ΔE=(ΔL)2 +(Δa)2+(Δb)2
Browning index (BI) was calculated from L, a, and b values as described by Maskan [26 (link)] following equation
Browning index (BI)=100(x0.31)0.17, where x =a+1.75L5.645L+(a3012b)
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