Alt infinity enzymatic assay kit

Serum alanine aminotransferase (ALT) was measured using an ALT Infinity Enzymatic Assay Kit (ThermoFisher Scientific Inc., Waltham, MA). Triglyceride (TG) was determined using a mouse Triglyceride Colorimetric Assay Kit (Cayman Chemical Company, CA). For histology, the harvested liver tissues were fixed in 10% buffered formalin for 2 weeks and then dehydration was done through graded alcohol series. The dehydrated tissues were cleared using xylene, embedded in paraffin, and sectioned at 4-5 μm slices. Hematoxylin and eosin (H&E) staining was performed in the micro-section to investigate the histopathological damage in the liver from all study groups. The images were reviewed and analyzed under microscope (Olympus 1X51, Olympus Corporation, Tokyo) at 20X magnification. The NAFLD activity score (NAS) was calculated from the sum of the individual scores for steatosis, inflammation and ballooning.

To analyze the liver injury and metabolic abnormalities in the liver, serum plasma alanine aminotransferase (ALT), serum glucose, serum insulin, serum and liver triglyceride (TG) were determined. The serum ALT was measured using an ALT infinity enzymatic assay kit (Thermo Fisher Scientific Inc., Waltham, MA), according to the instruction provided. Serum insulin was detected using an Ultra Sensitive Mouse Insulin ELISA Kit kit (Crystal Chem USA, IL), according to the instruction provided. Serum glucose assay was performed using a Sigma assay kit (Sigma-Aldrich Company, MI). TG assay was performed with TG assay kit (Cayman Chemical Company, CA).