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15n anthranilic acid

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15N anthranilic acid is a stable isotope-labeled compound. It is a white crystalline solid used as a research tool in various scientific applications.

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Lab products found in correlation

Gibberellic acid, by Merck Group (9 mentions) Jasmonic acid, by Merck Group (9 mentions) Thymine d4, by Merck Group (8 mentions) Testosterone d3, by Merck Group (8 mentions) Glutamic acid d5, by Merck Group (7 mentions) Sarcosine d3, by Merck Group (5 mentions) Trans zeatin, by Merck Group (5 mentions) N acetyl aspartic acid d3, by Merck Group (5 mentions) Tryptophan 15n2, by Merck Group (5 mentions) Methanol, by Avantor (4 mentions) 15n 2 tryptophan, by Merck Group (3 mentions) Trans zeatine, by Merck Group (3 mentions) Chloroform, by Avantor (2 mentions) Ms grade formic acid, by Merck Group (2 mentions) Hplc grade ammonium acetate, by Avantor (2 mentions) Trifluroacetic acid, by Agilent Technologies (2 mentions) Hplc grade acetonitrile, by Avantor (1 mentions) L tryptophan 15n2, by Merck Group (1 mentions) Esi l low concentration tuning mix, by Agilent Technologies (1 mentions) Formic acid, by Merck Group (1 mentions)

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Anthranilic acid (33 citations)

9 protocols using 15n anthranilic acid

1

HPLC-MS Chemical Standards Protocol

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High-performance liquid chromatography (HPLC) grade acetonitrile, methanol, and water were purchased from Burdick & Jackson (Morristown, NJ). The mass spectrometry grade formic acid and internal standards, including N-acetyl L-aspartic acid-d3, L-tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, 15N anthranilic acid, and testosterone-d3, were purchased from Sigma-Aldrich (St.Louis, MO). The ESI-L Low Concentration Tuning Mix (Agilent Technologies, Santa Clara, CA) was used to calibrate the mass spectrometer.
Jiao L., Maity S., Coarfa C., Rajapakshe K., Chen L., Jin F., Putluri V., Tinker L.F., Mo Q., Chen F., Sen S., Sangi-Hyghpeykar H., El-Serag H.B, & Putluri N. (2019). A prospective targeted serum metabolomics study of pancreatic cancer in postmenopausal women. Cancer prevention research (Philadelphia, Pa.), 12(4), 237-246.
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2

Comprehensive Metabolite Analysis by HPLC-MS

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High-performance liquid chromatography (HPLC)-grade ammonium acetate from Sigma, acetonitrile, methanol, chloroform, and water were procured from Burdick & Jackson (Morristown, NJ). Mass spectrometry-grade formic acid was purchased from Sigma-Aldrich (St Louis, MO).) Metabolite standards and internal standards, including N-acetyl Aspartic acid-d3, tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, 15N anthranilic acid, and Testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO).
Amara C.S., Ambati C.R., Vantaku V., Piyarathna D.W., Donepudi S.R., Ravi S.S., Arnold J.M., Putluri V., Chatta G., Guru K.A., Badr H., Terris M.K., Bollag R.J., Sreekumar A., Apolo A.B, & Putluri N. (2019). Serum metabolic profiling identified a distinct metabolic signature in Bladder Cancer Smokers: A key metabolic enzymes associated with patient survival. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 28(4), 770-781.
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3

Metabolite Extraction and Quantification

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HPLC grade ammonium acetate, acetonitrile, methanol, chloroform, ethanol, hexane, and water were procured from Burdick & Jackson (Morristown, NJ). MS grade formic acid, standard compounds and isotope labeled standards, including N-acetyl aspartic acid-d3, tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, 15N anthranilic acid, and testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO).
Ambati C.R., Vantaku V., Donepudi S.R., Amara C.S., Ravi S.S., Mandalapu A., Perla M., Putluri V., Sreekumar A, & Putluri N. (2018). Measurement of methylated metabolites using Liquid Chromatography-Mass Spectrometry and its biological application. Analytical methods : advancing methods and applications, 11(1), 49-57.
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4

Comprehensive Metabolomics Analytical Protocol

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Sources for reagents were: HPLC-grade acetonitrile and water (Burdick & Jackson); mass spectrometry-grade formic acid and ammonium acetate (Sigma-Aldrich); calibration solution containing multiple calibrants in a solution of acetonitrile, trifluroacetic acid, and water (Agilent Technologies); metabolite standards and internal standards, including N-acetyl Aspartic acid-d3, Tryptophan-15N2, Sarcosine-d3, Glutamic acid-d5, Thymine-d4, Gibberellic acid, Trans-Zeatine, Jasmonic acid, 15N Anthranilic acid, and Testosterone-d3 (Sigma-Aldrich).
Amobi-McCloud A., Muthuswamy R., Battaglia S., Yu H., Liu T., Wang J., Putluri V., Singh P.K., Qian F., Huang R.Y., Putluri N., Tsuji T., Lugade A.A., Liu S, & Odunsi K. (2021). IDO1 Expression in Ovarian Cancer Induces PD-1 in T Cells via Aryl Hydrocarbon Receptor Activation. Frontiers in Immunology, 12, 678999.
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5

Quantitative Metabolomics using HPLC-MS

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Acetonitrile, methanol, and water for high-performance liquid chromatography were purchased from Burdick and Jackson. Formic acid and internal standards, Gibberellic acid, Jasmonic acid, [15N]2-Tryptophan, Thymine-d4, [13C]-Creatinine, [15N] Arginine, [15N]-Anthranilic acid, and Testosterone-d3 (Supplementary Table 3), were purchased from Sigma Aldrich. U136 glucose was used purchased from Cambridge isotope laboratories, 5-Azacytidine, Adox, 680C91 were purchased from Sigma and GSK126 was procured from Cayman chemicals.
Vantaku V., Putluri V., Bader D.A., Maity S., Ma J., Arnold J.M., Rajapakshe K., Donepudi S.R., von Rundstedt F.C., Devarakonda V., Dubrulle J., Karanam B., McGuire S.E., Stossi F., Jain A.K., Coarfa C., Cao Q., Sikora A.G., Villanueva H., Kavuri S.M., Lotan Y., Sreekumar A, & Putluri N. (2019). Epigenetic loss of AOX1 expression via EZH2 leads to metabolic deregulations and promotes bladder cancer progression. Oncogene, 39(40), 6265-6285.
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6

HPLC-Based Quantification of Metabolites

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Reagents such as acetonitrile, methanol and water for high-performance liquid chromatography were purchased from Burdick and Jackson. Formic acid and internal standards, which included a mixture of Jasmonic acid, [15N]2-Tryptophan, Thymine-d4, Glutamic acid-d5, Gibberellic acid, Trans-Zeatin, [15N]-Anthranilic acid and Testosterone-d3, were purchased from Sigma-Aldrich. For UDP-N-Acetyl glucosamine quantification, [13C]6 N-acetyl glucosamine was used as an internal standard and the calibration curve for quantification was generated using the purified metabolite purchased from Sigma-Aldrich.
Kaushik A.K., Shojaie A., Panzitt K., Sonavane R., Venghatakrishnan H., Manikkam M., Zaslavsky A., Putluri V., Vasu V.T., Zhang Y., Khan A.S., Lloyd S., Szafran A.T., Dasgupta S., Bader D.A., Stossi F., Li H., Samanta S., Cao X., Tsouko E., Huang S., Frigo D.E., Chan L., Edwards D.P., Kaipparettu B.A., Mitsiades N., Weigel N.L., Mancini M., McGuire S.E., Mehra R., Ittmann M.M., Chinnaiyan A.M., Putluri N., Palapattu G.S., Michailidis G, & Sreekumar A. (2016). Inhibition of the hexosamine biosynthetic pathway promotes castration-resistant prostate cancer. Nature Communications, 7, 11612.
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7

Metabolomic Profiling of Eosinophilic Esophagitis

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Using mass spectrometry, 48 metabolites were measured and compared between children with active EoE and unaffected controls.
Metabolites and internal standards, including N-acetyl aspartic acid-d3, [15N]2 Tryptophan, Glutamic acid-d5, Thymine-d4, Gibberellic acid, Trans-Zeatine, Jasmonic acid, [15N] Anthranilic acid, and Testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO).
Moye L.M., Liu Y., Coarfa C., Putluri N, & Rhoads J.M. (2019). Plasma Urea Cycle Metabolites May Be Useful Biomarkers in Children With Eosinophilic Esophagitis. Frontiers in Pediatrics, 6, 423.
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8

Metabolomic Analysis of Plant Hormones

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High-performance liquid chromatography (HPLC)-grade acetonitrile, methanol, and water were procured from Burdick & Jackson (Morristown, NJ). Mass spectrometry-grade formic acid was purchased from Sigma-Aldrich (St Louis, MO). Calibration solution containing multiple calibrants in a solution of acetonitrile, trifluroacetic acid, and water was purchased from Agilent Technologies (Santa Clara, CA). Metabolites and internal standards, including N-acetyl Aspartic acid-2H3, Tryptophan-15N2, Sarcosine-2H3, Glutamic acid-2H5, Thymine-2H4, Gibberellic acid, Trans-Zeatine, Jasmonic acid, Anthranilic acid 15N, and Testosterone-2H3, were purchased from Sigma-Aldrich (St. Louis, MO).
Colleluori G., Aguirre L., Phadnis U., Fowler K., Armamento-Villareal R., Sun Z., Brunetti L., Park J.H., Kaipparettu B.A., Putluri N., Auetumrongsawat V., Yarasheski K., Qualls C, & Villareal D.T. (2019). Aerobic plus Resistance Exercise in Obese Older Adults Improves Muscle Protein Synthesis and Preserves Myocellular Quality Despite Calorie Restriction. Cell metabolism.
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9

Comprehensive Metabolite Profiling Approach

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High-performance liquid chromatography (HPLC)-grade ammonium acetate, acetonitrile, methanol, chloroform and water were procured from Burdick & Jackson (Morristown, NJ). MS grade formic acid, standards and internal standards, N-acetyl aspartic acid-d3, tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, anthranilic acid-15N, and testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO). Mixture of LPC 17:0/0:0, PG 17:0/17:0, PE 17:0/17:0, PC 17:0/17:0, TAG 17:0/17:0/17:0, SM 18:1/17:0, MAG 17:0, DAG 16:0/18:1, CE 17:0, ceramide 18:1/17:0, PA 17:0, PI 17:0/20:4, and PS 17:0/17:0. were obtained from Avanti polar lipids (USA) (Supplementary Table S1).
Vantaku V., Dong J., Ambati C.R., Perera D., Donepudi S.R., Amara C.S., Putluri V., Shiva Shankar R., Robertson M.J., Piyarathna D.W., Villanueva M., von Rundstedt F.C., Karanam B., Ballester L.Y., Terris M.K., Bollag R.J., Lerner S.P., Andrea A.B., Villanueva H., Lee M., Sikora A.G., Lotan Y., Sreekumar A., Coarfa C, & Putluri N. (2019). Multi-omics integration analysis robustly predicts high-grade patient survival and identifies CPT1B effect on fatty acid metabolism in Bladder Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(12), 3689-3701.
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