1 2 di o lauryl rac glycero 3 glutaric acid 6 methylresorufin ester

Dulbecco’s modified eagle medium (DMEM-F12), fetal bovine serum (FBS), penicillin and streptomycin solution, and trypsin-ethylenediaminetetra acetic acid were purchased from Life Technologies (Eugene, OR, USA). Phosphate buffered saline (PBS) was obtained from Biochrom AG (Berlin, Germany). HMG-CoA reductase assay kit was purchased from Sigma Chemical Co. (St. Louis, MO, USA). (1α,2α(n)-³H) Cholesterol (specific activity, 49 Ci/mmol) was purchased from Perkin-Elmer (Wellesley, MA, USA). NPC1L1 antibody was bought from Novus Biologicals (Littleton, CO, USA). Ezetimibe was purchased from Schering-Plough Research Institute (Kenilworth, NJ, USA). Folin-Ciocalteu reagent, sodium bicarbonate, 1,2-di-O-lauryl-rac-glycero-3-glutaric acid 6’-methylresorufin ester, taurocholic acid sodium salt hydrate, glycodeoxycholic acid, taurodeoxycholic acid, and hydrazine hydrate solution were received from Sigma-Aldrich Co. (St. Louis, MO, USA). All other chemical reagents used in this study were obtained from commercial sources.