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Colorimetric assay kit

Manufactured by Cayman Chemical
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The Colorimetric assay kit is a laboratory tool used to measure the concentration of a specific analyte in a sample. It provides a quantitative analysis by generating a colored reaction, which can be detected and measured using a spectrophotometer or colorimeter. The kit includes all the necessary reagents and materials required to perform the assay.

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Lab products found in correlation

Spectramax m3, by Molecular Devices (3 mentions) Synergy ht multi mode microplate reader, by Agilent Technologies (2 mentions) Colorimetric assay kit, by Abcam (2 mentions) Colorimetric assay kit, by Biodiagnostic (2 mentions) Ultra sensitive mouse insulin elisa kit, by Crystal Chem (2 mentions) Fluorometric assay kit, by Cayman Chemical (2 mentions) Colorimetric assay kit, by Cell Biolabs (2 mentions) Enzyme immunoassay kit, by Cayman Chemical (2 mentions) Polytron homogenizer, by Qiagen (2 mentions) C reactive protein (crp), by Aviva Systems Biology (1 mentions) Phosphotungstic acid mg2 method, by Randox (1 mentions) Glutathione assay kit, by Cayman Chemical (1 mentions) Oxiselect protein carbonyl elisa kit, by Cell Biolabs (1 mentions) 4 hydroxynonenal 4 hne assay kit, by Cell Biolabs (1 mentions) Celecoxib capsule, by Pfizer (1 mentions) Sciex 4000 qtrap mass spectrometer, by AB Sciex (1 mentions) Multiquant software, by Thermo Fisher Scientific (1 mentions) Synergy 2 microplate reader, by Agilent Technologies (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Bio plex 200 system, by Bio-Rad (1 mentions)

Spelling variants of this product

Colorimetric assay (69 citations) Creatinine (urinary) Colorimetric Assay Kit (26 citations) Hemoglobin Colorimetric Assay Kit (18 citations) Aspartate aminotransferase colorimetric activity assay kit (4 citations) BHB colorimetric assay kit (4 citations) Colorimetric assays kits (2 citations) ALT and AST colorimetric activity assay kits (2 citations) Creatinine urinary/serum Colorimetric Assay Kits (2 citations) Griess reaction colorimetric assay kit (2 citations) TGs Colorimetric Assay KIT (2 citations)

177 protocols using colorimetric assay kit

1

Inflammatory and Antioxidant Markers in BAL and Plasma

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In BAL fluid supernatant (n=6–8 per group), TNFα level was measured with a commercially available ELISA kit (Duo Set; R & D Systems, Minneapolis, MN, USA), and catalase activity was quantified by using a colorimetric assay kit (Cayman Chemical Company, Ann Arbor, MI, USA).
In plasma (n=6–8 per group), besides measuring TNFα level and catalase activity as described earlier, the levels of CRP (GenWay Biotech, Inc., San Diego, CA, USA), fibrinogen (Molecular Innovation, Southfield, MI, USA), and PAI-1 (Molecular Innovation) were measured by using ELISA kits. The total antioxidant activity was quantified with a colorimetric assay kit (Cayman Chemicals, Ann Arbor, MI, USA).
Nemmar A., Al-Salam S., Beegam S., Yuvaraju P, & Ali B.H. (2017). The acute pulmonary and thrombotic effects of cerium oxide nanoparticles after intratracheal instillation in mice. International Journal of Nanomedicine, 12, 2913-2922.
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2

Metabolic Biomarker Quantification Protocol

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Plasma was collected following an overnight fast. Glucose
concentrations were measured using a glucometer. Plasma insulin and
TNFα concentrations were assayed using commercial ELISA kits.
Triglyceride levels were measured using a colorimetric assay kit (Cayman
Chemical). Homeostatic model assessment for insulin resistance (HOMA-IR) was
calculated using the following formula: [fasting glucose (mM/l) x fasting
insulin (IU/ml)]/22.5.
Lee C.H., Kim H.J., Lee Y.S., Kang G.M., Lim H.S., Lee S.H., Song D.K., Kwon O., Hwang I., Son M., Byun K., Sung Y.H., Kim S., Kim J.B., Choi E.Y., Kim Y.B., Kim K., Kweon M.N., Sohn J.W, & Kim M.S. (2018). Hypothalamic Macrophage Inducible Nitric Oxide Synthase Mediates Obesity-Associated Hypothalamic Inflammation. Cell reports, 25(4), 934-946.e5.
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3

Lipoprotein Quantification Methods Protocol

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The total cholesterol, triglycerides and glucose plasma concentrations were determined by enzymatic colorimetric methods (Randox Laboratories Ltd., Crumlin, Antrim, U.K.). Sphingomyelin (SM) concentrations were assessed by a commercial colorimetric test (Cayman Chemical, Ann Arbor, MI, USA). The phosphotungstic acid−Mg2+ method (Randox Laboratories Ltd., Crumlin, Antrim, U.K.) was used to precipitate the apo B-containing lipoproteins (i.e., Very Low-Density Lipoproteins/Low-Density Lipoprotein); then HDL-C and HDL-Tg plasma concentrations were determined in the supernatant fraction by the same enzymatic colorimetric methods (Randox Laboratories Ltd., Crumlin, Antrim, U.K.), whereas HDL-phospholipids (HDL-Pho) were determined with a commercial kit (Wako Chemicals, Richmond, VA, USA).
HDL-SM and non−HDL-SM were determined by analytical ultracentrifugation. Plasma samples were adjusted at a density of 1.063 g/mL with solid KBr. The bottom (HDL) and top (non-HDL lipoproteins) of the tubes after ultracentrifugation were quantitatively recovered and analyzed using the same colorimetric assay kit mentioned above (Cayman Chemical, Ann Arbor, MI, USA). All the determinations were performed following the manufacturer’s instructions.
Dorantes-Morales A., Estrada-Luna D., Bautista-Pérez R., Betanzos-Cabrera G., Luna-Luna M., Flores-Castillo C., Vargas-Alarcón G., Fragoso J.M., Pérez-Méndez Ó, & Carreón-Torres E. (2020). Microencapsulated Pomegranate Modifies the Composition and Function of High-Density Lipoproteins (HDL) in New Zealand Rabbits. Molecules, 25(14), 3297.
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4

Nitrite/Nitrate Quantification via Colorimetric Assay

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The NOx (nitrite/nitrate) content was measured using a colorimetric assay kit according to manufacturer’s instructions (Cayman Chemical Company, Ann Arbor, MI) and as detailed (Misko, Schilling, Salvemini, Moore, & Currie, 1993 (link)).
McGee M.A, & Abdel-Rahman A.A. (2015). Ethanol Attenuates Peripheral NMDAR-Mediated Vascular Oxidative Stress and Pressor Response. Alcohol (Fayetteville, N.Y.), 49(5), 499-506.
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5

Plasma Metabolite Analysis by HPLC-MS/MS

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l-Carnitine, trimethyllysine, γ-butyrobetaine, and acetylcarnitine were analyzed in plasma by HPLC-MS/MS, as described by Vernez et al. (17 (link)) with some modifications (18 (link)). The ketone body d-β-hydroxybutyrate was analyzed in plasma by the Colorimetric Assay Kit from Cayman Chemical Company (item no. 700,190).
Dankel S.N., Bjørndal B., Lindquist C., Grinna M.L., Rossmann C.R., Bohov P., Nygård O., Hallström S., Strand E, & Berge R.K. (2021). Hepatic Energy Metabolism Underlying Differential Lipidomic Responses to High-Carbohydrate and High-Fat Diets in Male Wistar Rats. The Journal of Nutrition, 151(9), 2610-2621.
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6

Nitrite and Nitrate Quantification

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The released nitrite and nitrate in the culture medium were determined using a commercially available Colorimetric Assay Kit following the manufacturer’s instruction (Cayman Chemical Company, Ann Arbor, MI, USA).
Lundquist I., Mohammed Al-Amily I., Meidute Abaraviciene S, & Salehi A. (2016). Metformin Ameliorates Dysfunctional Traits of Glibenclamide- and Glucose-Induced Insulin Secretion by Suppression of Imposed Overactivity of the Islet Nitric Oxide Synthase-NO System. PLoS ONE, 11(11), e0165668.
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7

Metabolic Biomarkers in Fasting and Fed Mice

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Serum triglycerides (TG), free fatty acids (FFA), glucose, and insulin in 12-h fasted and in 1-h post-prandial (fed) mice were determined using commercially available kits. TG, FFA, and glucose kits were from Wako. Insulin was determined using enzyme-linked immunoassay-based, ultra-sensitive mouse insulin kit (Crystal). Determination of serum β-hydroxybutyrate (for ketone body analysis) from 6-h fasted mice was done using colorimetric assay kit from Cayman. WBC counts in blood freshly collected by cardiac puncture in the presence of 10 mM EDTA were determined using Advia hematology system.
Arif A., Terenzi F., Potdar A.A., Jia J., Sacks J., China A., Halawani D., Vasu K., Li X., Brown J.M., Chen J., Kozma S.C., Thomas G, & Fox P.L. (2017). EPRS is a critical mTORC1-S6K1 effector that influences adiposity in mice. Nature, 542(7641), 357-361.
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8

Sublingual Blood Sampling and Corticosterone Analysis

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Blood was taken sublingually under general anesthesia (5% isoflurane). Baseline samples were collected 2 days prior to initiating the task protocol, at the same time of day that blood would be collected on contextual fear conditioning testing days, which occurred 25 min post-acquisition training and also the same length of time post-retention testing. Approximately, 1 ml samples were taken using ethylenediaminetetraacetic acid (EDTA)-primed vials (Thermo Fisher Scientific, UK), which were then placed on ice and centrifuged at 3000×g at 4 °C for 20 min. Plasma was aliquoted into Eppendorf tubes for corticosterone level analysis. Corticosterone (Cat No. ADI-901-097, Enzo Life Sciences, Devon, UK; RRID: AB_2307314) and leptin (Cat No. ADI-900-015A, Enzo Life Sciences, UK) quantification was done using enzyme-linked immunoassay (ELISA) kits with glucose quantification that was done using a colorimetric assay kit (Cat No. 10009582, Cayman Chemical, Michigan, USA), all according to the manufacturer’s instructions. The plasma corticosterone and leptin concentration levels are given as ng/ml, while glucose plasma concentration is given as mg/ml.
Mantanona C.P., Alsiö J., Elson J.L., Fisher B.M., Dalley J.W., Bussey T, & Pienaar I.S. (2019). Altered motor, anxiety-related and attentional task performance at baseline associate with multiple gene copies of the vesicular acetylcholine transporter and related protein overexpression in ChAT::Cre+ rats. Brain Structure & Function, 224(9), 3095-3116.
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9

Quantifying Oxidative Stress Markers

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As markers of oxidative stress, levels of protein-bound oxidized tyrosine moieties, 3-nitrotyrosine (NY) and o, o’-dityrosine (DY) were quantified by isotope dilution liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) as described previously [47 (link)] in tissue extracts from muscle and liver and lipidperoxidation marker Thiobarbituric Acid Reactive Substances (TBARS) utilizing commercially available colorimetric assay kit (Cayman Chemicals, Ann Arbor, MI) in tissue extracts from VAT, muscle and liver. For oxidized tyrosine quantification, tissue extracts were delipidated, precipitated and proteins hydrolyzed along with known amounts of isotopically labeled internal standards13C6-Y and 13C6-NY, 13C6-ClY, and 13C12-o, o’-DY. The extracts were then subjected to solid-phase extraction and the amount of oxidized amino acids was quantified by HPLC-ESI-MS/MS. The levels of the oxidized amino acids in each sample were then normalized to the amino acid tyrosine content in the respective samples and expressed as the ratio of the oxidized product over the total tyrosine. Intraassay coefficients of variation for NY and DY were 8.47% and 8.16%, respectively. For TBARS assay, total protein of the tissue was isolated and assay carried out as per manufacturer’s recommendation and Intra- and inter-assay coefficients were 5.6% and 10.2%, respectively.
Puttabyatappa M., Martin J.D., Andriessen V., Stevenson M., Zeng L., Pennathur S, & Padmanabhan V. (2019). Developmental Programming: Changes in Mediators of Insulin Sensitivity in Prenatal Bisphenol A-treated Female Sheep. Reproductive toxicology (Elmsford, N.Y.), 85, 110-122.
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10

Urinary 8-isoprostane measurement

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We measured 8-isoprostane as previously described.23 (link) In short, 24-h urine samples were collected in the metabolic cages at the end of the experimental period. We measured the urinary concentrations of 8-isoprostane using a colorimetric assay kit (Cayman Chemical Company, Ann Arbor, USA). This was normalized to the urinary creatinine concentration.
Potthoff S., Stamer S., Grave K., Königshausen E., Sivritas S., Thieme M., Mori Y., Woznowski M., Rump L, & Stegbauer J. (2016). Chronic p38 mitogen-activated protein kinase inhibition improves vascular function and remodeling in angiotensin II-dependent hypertension. Journal of the Renin-Angiotensin-Aldosterone System: JRAAS, 17(3), 1470320316653284.
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