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5 protocols using hydrogen peroxide

1

Synthesis and Characterization of Carbon Nanotube-Based Nanocomposites

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CNT sheets were purchased from
Suzhou Jernano Carbon Co., Ltd., China. Hydrochloric acid (HCl, 38%)
and hydrogen peroxide (H2O2, ≥30%) were
purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd., China.
Sodium hydroxide (NaOH, AR) was purchased from Sinopharm Chemical
Reagent Co., Ltd., China. Polyvinyl alcohol (PVA, alcoholysis degree
97.5–99%), dimethyl sulfoxide (DMSO, 99%), dopamine hydrochloride
(DA), tris-hydrochloride buffer (Tris-HCl, 1 M, pH = 8.5), and gold
chloride trihydrate (HAuCl4·3H2O, ≥99.9%)
were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.,
China. Potassium bromide (KBr) was purchased from Sigma-Aldrich, USA.
Rhodamine 6G (R6G, 99%) was purchased from Thermo Fisher Scientific,
China.
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2

Activated Carbon Modification and Catalysis

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All the chemicals and reagents were of analytical grade and were used without further purification.
Commercial activated carbon was purchased from France Arkema CECA (3sw, particle size: 250–300 mesh, N2‐BET: 980 m2 g−1, ash content: <3 %). Nitric acid (HNO3, 65.0∼68.0 wt%, Sinopharm Chemical Reagent Co., Ltd.), Hydrogen peroxide (H2O2, 30 wt%, Shanghai Lingfeng Chemical Reagent Co., Ltd.), Oxalic acid dehydrate (C2H2O4⋅2H2O, ≥99.9 wt%, Shanghai Lingfeng Chemical Reagent Co., Ltd.), Ammonia solution (NH4OH, 25.0∼28.0 wt%, Hangzhou Longshan Fine Chemical Co., Ltd.), 1‐Chloro‐4‐nitrobenzene (C6H4ClNO2, 98 wt%, Aladdin Chemistry Co., Ltd.),Palladium chloride (PdCl2, Pd content ≥59.5 wt%, Deqing Degong Chemical Engineering Co., Ltd.), Deionized water (resistivity >18 Ω cm−1).
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Fabrication of Superhydrophobic Surfaces

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The chemicals used in the fabrication of superhydrophobic surfaces are as following: silver nitrate(99.8%, Aladdin, China); acetone(99.5%, Sinopharm Chemical Reagent Co. Ltd, China); ethanol(99.7%, Changshu Hong Sheng fine chemical Co. Ltd, China); sulphuric acid(98%, Sinopharm Chemical Reagent Co. Ltd, China); hydrogen peroxide(30%, Shanghai Ling Feng Chemical Reagent Co. Ltd, China);hydrofluoric acid(40%, Sinopharm Chemical Reagent Co. Ltd, China); ferric nitrate(98.5%, Sinopharm Chemical Reagent Co. Ltd, China). Perfluoro-1,1,2,2-tetrahydrooctyltrichlorosilane(97%, Alfa Aesar, US).
The silicon wafer was purchased from the Zhejiang Li Jing Photoelectric Technology Co. Ltd. The wafer is P type and its resistivity is in the range of 0.1 ~ 1 Ω·cm.
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4

Chemiluminescent Biosensor Reagent Preparation

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All reagents were used without further purification, and deionized distilled water was used throughout the experiments. A stock solution of Na3Au(SO3)2 was purchased from Changzhou Institute of Chemical Research (Changzhou, China). L012 was bought from Wako Chemical USA Inc. (Richmond, VA, USA). PLD and COD were purchased from Sigma-Aldrich Co., Ltd. (Shanghai, China), lecithin was obtained from Tokyo Chemical Industry Co., Ltd., and hydrogen peroxide was purchased from Shanghai Ling Feng Chemical Reagent Co., Ltd. (Shanghai, China). A 0.01 M stock solution of luminol was kept at 4 °C in a dark bottle when not in use. hydrogen peroxide solutions were prepared by making appropriate dilutions from a 30% hydrogen peroxide solution. lecithin was dissolved in trichloromethane, and the solvent was evaporated in nitrogen. PLD and COD were dissolved in 10 mM phosphate buffered solution (PBS, pH 7.4) and diluted to 10 U/mL and 4 U/mL, respectively.
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5

Polyimide-Based Biosensor Fabrication

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Polyimide (PI) films with a thickness of 125 μm (Kapton®) were purchased from Weishengte Technology Co., Ltd. (Beijing, China). Polydimethylsiloxane (SycgardTM 184 silicone) was acquired from Dow Corning (Midland City, MI, USA). Dihydrogen hexachloroplatinate hexahydrate (H2PtCl6·6H2O) was obtained from Energy Chemical (Shanghai, China). glucose oxidase (GOx, EC 1.1.3.4, 123 kU/g) was purchased from Toyobo Co., Ltd. (Shanghai, China). Bovine Serum Albumin (BSA), lactate oxidase, sucrose invertase and ethanol oxidase were acquired from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Hydrogen peroxide was purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. Chitosan and glutaraldehyde (25%) were obtained from Aladdin. Cyclohexane, potassium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, glucose, sucrose, ethanol, lactate, methanol, mannose, xylose, galactose, citric acid and CH3COOH were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All of our experiments used deionized (DI) water. All reagents were directly used without further purification.
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