D-luciferin was purchased from Abcam (Cambridge, MA) and was resuspended in PBS at a concentration of 100 mg/mL. The mice were intraperitoneally injected with 150 mg/kg body weight D-luciferin and imaged 10 minutes thereafter using an IVIS® Spectrum optical imaging system fitted with an XGI-8 Gas Anesthesia System (Caliper Life Sciences, Hopkinton, MA). Bioluminescent images were acquired using the auto-exposure function. Analysis of the signal intensities and image comparisons were performed using Living Image® Software (Caliper Life Sciences).
Ivis spectrum optical imaging system
Manufactured by PerkinElmer
Sourced in United States
The IVIS Spectrum is an optical imaging system designed for in vivo analysis of bioluminescent and fluorescent signals in small animal models. It provides quantitative data on biological processes and gene expression. The system utilizes highly sensitive charge-coupled device (CCD) cameras to capture real-time images and data.
Automatically generated - may contain errors
Lab products found in correlation
Living image software, by PerkinElmer (7 mentions)
D luciferin, by PerkinElmer (5 mentions)
Xgi 8 gas anesthesia system, by PerkinElmer (4 mentions)
D luciferin, by Abcam (3 mentions)
Living image software 4, by PerkinElmer (3 mentions)
D luciferin, by GoldBio (2 mentions)
D luciferin potassium salt, by GoldBio (2 mentions)
Analyze 12, by PerkinElmer (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Laser scanning confocal microscope, by Nikon (1 mentions)
Living image software version 4, by PerkinElmer (1 mentions)
Ellman s reagent, by Thermo Fisher Scientific (1 mentions)
Ivis living imaging software, by PerkinElmer (1 mentions)
24 protocols using ivis spectrum optical imaging system
1
In Vivo Bioluminescence Imaging
2
In Vivo Bioluminescence Imaging of Luciferase
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D-luciferin was purchased from Abcam (Cambridge, MA) and resuspended at 100 mg/mL in PBS. For Fluc imaging, mice were injected i.p. with 150 mg/kg body weight of D-luciferin and imaged 10 minutes later using the IVIS® Spectrum optical imaging system fitted with an XGI-8 Gas Anesthesia System (Caliper Life Sciences, Hopkinton, MA). Bioluminescent images were acquired using the autoexposure function. Data analysis for signal intensities and image comparisons were performed using Living Image® software (Caliper Life Sciences).
3
Retrovirally Induced Glioblastoma Mouse Model
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Mice of both sexes (6–8 weeks old) were used for viral injections. 1 μL of PDGF-BB – IRES – Cre retrovirus (106/mL titer) was injected into the subcortical white matter at the following coordinates (with bregma as reference): 2.2 mm lateral, 2.2 mm rostral and 2 mm deep, at a flow rate of 0.33 μL/minute. Tumor growth in mCherry-Luciferase+ mice was monitored by bioluminescent imaging on an IVIS Spectrum Optical Imaging System (Caliper) once weekly as previously described(Lei et al., 2011 (link)). Retrovirally induced tumors from 2 male N1IC+/TP53fl/fl and 2 male TP53fl/fl were dissected at end-stage for single cell RNA sequencing (scRNA-seq) analysis, as described below. For orthotopic cell transplantation experiments, 6-week-old C57BL/6 male mice were injected with 100,000 cells (p53–1 or N1IC-1, passage 10, isolated from littermates) resuspended in 2 μL PBS at the following coordinates (with bregma as reference): 2.5 mm lateral, 2 mm rostral and 2.5 mm deep, and at a flow rate of 0.25 μL/min. Mice were sacrificed either at 30 days post injection or when endstage criteria were met, as indicated in the corresponding Figures.
4
Bioluminescence Imaging of Tumors
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D-luciferin was purchased from Abcam (Cambridge, MA, USA) and was resuspended in PBS at a concentration of 100 mg/mL. The mice were intraperitoneally injected with 150 mg/kg per total body weight D-luciferin and imaged 10 min thereafter using an IVIS® Spectrum optical imaging system fitted with an XGI-8 Gas Anesthesia System (Caliper Life Sciences, Hopkinton, MA, USA). Bioluminescent images were acquired using the exposure function and all the parameters were the same in every measurement. We measured the total flux (photons/s) of the tumor bioluminescence signal by placing a region of interest at the tumor using Living Image® Software (Caliper Life Sciences).
5
Bioluminescent Imaging of Luciferase
6
Bioluminescent Imaging of Mice
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The mice were intraperitoneally injected with D-luciferin (Cambridge, MA) at a dose of 150 mg/kg of body weight and exposed to an IVIS Spectrum optical imaging system for 10 min to acquire bioluminescent images, which were analyzed to obtain signal intensities and were compared among the different groups using Living Image Software (Caliper Life Sciences).
7
In Vivo Biodistribution of EVs
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M2-Mφ or MSC-EVs were labeled with sulfo-Cy7-NHS ester according to the manufacturer's instructions (New Research Biosciences Co., Ltd., Xi'an, China). The unbound dyes were removed using an Exosome Spin Column (MW3000, Invirogen). Cy7-labeled M2-EVs or MSC-EVs (Cy7-EVs, 80 μg in 100 μL PBS per mouse) and an equal amount of free Cy7 (dye alone, negative control) were intravenously injected (i.v.) into C57/BL6 mice via the tail vein. At the indicated time points after injection, the mice were sacrificed by an overdose of pentobarbital sodium anesthesia, and the organs, including the hearts, lungs, livers, kidneys, and spleens, were collected and observed using an IVIS Spectrum optical imaging system (Perkin Elmer, Waltham, MA, USA). The intensity of fluorescence was quantified by Analyze 12.0 software (Perkin Elmer). To detect the distribution of EVs in organs, frozen sections of lung, liver, and spleen were made and costained with anti-CD68 (Abcam, ab201340) and DAPI (Sigma). Digital images of the stained sections were captured using a confocal laser scanning microscope (Nikon).
8
Bioluminescence Imaging for Tumor Burden
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For tumor burden monitoring, mice were injected 3 mg D-Luciferin (Goldbio, St. Louis, MO, USA) i.p. for bioluminescence imaging (BLI) following a 5 min uptake period. An IVIS Spectrum Optical Imaging system (Perkin Elmer) was used to acquire BLI scans for 60 s or 1 s and signals were normalized for the luciferase activity [photons/second]. ROI scans were analyzed using Living Image Software 4 (Perkin Elmer), by drawing regions of interest (ROI) around the whole animal and corrected for background signal.
9
Leukemia Progression Monitoring in Mice
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All leukemia models were introduced by i.v. injection and transplanted into nonirradiated secondary recipient experimental animals. BALB/c mice were used for the WEHI-3B leukemia model (0.5 × 106/cells/mouse) and C57BL/6J mice for MLL/AF9-dsRed (0.2 × 106/cells/mouse). Leukemia progression was assessed by fluorescence (MLL/AF9 dsRed) using the IVIS-Spectrum Optical Imaging System (Caliper, PerkinElmer). Mice were shaved to reduce light attenuation.
10
Intranasal Delivery of Fluorescent LNPs
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Adult male and female nude mice were purchased from Charles River. The mice were maintained in the University of Manitoba Central Animal Care Facility under a temperature-controlled environment with a 12-hour dark-light cycle and unlimited access to food and water. All experiments complied with the Canadian Council on Animal Care guidelines and the protocol (#22-040) approved by the University of Manitoba Animal Care Committee. Before intranasal administration, the mice were anesthetized with 2.5% isoflurane. The LNP formulation (LNP-PEP and LNP-siACE2) in PBS was administered with a Hamilton microsyringe with a blunt needle tip inserted 3 mm into the nostril (5 µL per nostril; 4 mg/Kg final dose). Serial images of the fluorescent LNPs were taken at different time intervals (5, 10, 15, 30, 60, 120, 180, and 1440 minutes) using a PerkinElmer/Caliper IVIS Spectrum optical imaging system (Ex/Em: 745/800 nm; Binning Factor: 4 and Exposure Time: 10 seconds). The fluorescence activity at different time points in specified regions of interest (ROI) was estimated as Total Radiant Efficiency [p/s]/[µW/cm²] using PerkinElmer Living Image® software.
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