Cd15 hi98

Manufactured by BD

The CD15 (HI98) is a laboratory equipment product developed by BD. It is a device designed for the detection and measurement of CD15, a cell surface marker. The core function of the CD15 (HI98) is to provide accurate and reliable data on the expression of CD15 in biological samples, without making any interpretations or extrapolations about its intended use.

Automatically generated - may contain errors

Lab products found in correlation

Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Cd38 90, by BioLegend (1 mentions) F4 80 bm8, by BioLegend (1 mentions) Ly6g 1a8, by BD (1 mentions) Cd86 bu63, by BioLegend (1 mentions) Gapdh d16h11, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

CD15 APC (HI98; (2 citations) CD15 (clone HI98, (2 citations) Anti-CD15-PE-Cy7 (clone HI98) (2 citations)

2 protocols using cd15 hi98

Multicolor Flow Cytometry Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorophore-conjugated anti-human CD3 (UCHT1), CD4 (SK3), CD8 (RPA-T8), CD14 (MφP9), CD25 (M-A251), CD33 (WM53), CD127 (HIL-7R-M21) and HLA-DR (G46-6) antibodies were purchased from BD Biosciences. CD15 (HI98, BD Bioscience) was conjugated with PerCP using the Expedeon Lightning-Link kit. Anti-human CD11b (CD11B29) antibody and LIVE/DEAD Fixable Blue Dead Cell Stain Kit for UV excitation were obtained from ThermoFisher Scientific.

Bayik D., Lauko A.J., Roversi G.A., Serbinowski E., Acevedo-Moreno L.A., Lanigan C., Orujov M., Lo A., Alban T.J., Kim A., Silver D.J., Nagy L.E., Brown J.M., Allende D.S., Aucejo F.N, & Lathia J.D. (2020). Hepatobiliary malignancies have distinct peripheral myeloid-derived suppressor cell signatures and tumor myeloid cell profiles. Scientific Reports, 10, 18848.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies and their corresponding clones used for flow cytometry (FC): anti-human CD11b (M1/70), CD33 (WM53), HLA-DR (G46-6), CD14 (M5E2), and CD15 (HI98) were obtained from BD Biosciences (Woburn, MA). Zombie NIR was used as a cell viability dye and CD86 (BU63) was obtained from (BioLegend, San Diego, CA). FC antibodies used to detect mouse MDSCs and macrophages were Gr1 (Ly6G/Ly6C, RB6-8C5), CD11b (M1/170), Ly6C (AL-21), and Ly6G (1A8) obtained from BD Biosciences, F/480 (BM8), CD38 (90) obtained from BioLegend, and EGR2 (ERONGR2) procured from Thermo Fisher Scientific (Waltham, MA). Antibodies against TLR7 (D7), TLR9 (D2C9), IL6 (D3K2N), phospho-NFκB p65^Ser536 (93H1), total NFκB (C22B4), phospho-STAT3^Tyr705 (D3A7) and total STAT3 (D3Z26) were obtained from Cell Signaling Technology (Danvers, MA). GAPDH (D16H11) was used for loading control and was obtained from Cell Signaling Technology.

Ghosh C.C., Heatherton K.R., Connell K.P., Alexander I.S., Greer D.A., LaPorte J., Guha P., Cox B.F, & Katz S.C. (2022). Regional infusion of a class C TLR9 agonist enhances liver tumor microenvironment reprogramming and MDSC reduction to improve responsiveness to systemic checkpoint inhibition. Cancer Gene Therapy, 29(12), 1854-1865.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!