Ascend 600 mhz instrument

The HRESIMS analyses were performed on a Waters Xevo G2-XS QTof mass spectrometer (Waters Corp., Milford Massachusetts, America). NMR spectra were recorded on an Ascend 600 MHz instrument (Bruker-Biospin, Billerica, MA, America). The analytical experiments were performed on a Shimadzu LC-20AT HPLC system (Shimadzu Corp., Kyoto, Japan) equipped with a Shimadzu InertSustain C18 column (4.6 I.D. × 250 mm, 5 μm, S/N 6LR98081). A Hanbon NP700 semipreparative HPLC (Hanbon Sci. & Tech., Jiangsu, China) equipped with a Shimadzu InertSustain C18 column (10 I.D. × 250 mm, 5 μm, S/N 7ER43006) was used for purifying compounds. Biological assays were monitored on a BioTek ELx808 microplate spectrophotometer (BioTek Instruments, Inc., Winooski, America), a BD FACSCalibur flow cytometry (BD Biosciences, Franklin Lakes, America) and an Olympus BX-51 Fluorescence Microscopy (Olympus Corporation, Tokyo, Japan).

NMR experiments were performed using the Ascend 600 MHz instrument (Bruker, Bremen, Germany) equipped with 5 mm BBO probe at 298 K. Before 1H and 13C spectra acquisition, examined samples were dissolved in CDCl₃. ¹H–¹H Double Quantum Filtered COSY and ¹H–¹³C Multiplicity Edited HSQC techniques were employed to facilitate assignment of proton and carbon resonances. Acquisition and processing of considered spectra were performed using TopSpin 3.5 and IconNMR software (Bruker, Bremen, Germany). Tetramethylsilane was used as an internal standard for calibration of ¹H and ¹³C chemical shifts. NMR data are gathered in Table 1S and 2S (see supplementary materials).