αcd8 fitc

Manufactured by BioLegend

αCD8-FITC is a monoclonal antibody conjugated with fluorescein isothiocyanate (FITC) that binds to the CD8 antigen expressed on the surface of T cells. It is used for the identification and enumeration of CD8+ T cells by flow cytometry.

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Lab products found in correlation

Anti mouse pd 1, by BioXCell (1 mentions) αcd38 pe cy7, by BioLegend (1 mentions) Anti mouse ctla 4, by BioXCell (1 mentions) Dnase, by Roche (1 mentions) Liberase, by Roche (1 mentions) Mouse tumor dissociation kit, by Miltenyi Biotec (1 mentions) αpdl1 pe cy7, by BD (1 mentions) Foxp3 af700, by Thermo Fisher Scientific (1 mentions) αcd4 percp cy5, by BD (1 mentions) αcd49b pe cy7, by BD (1 mentions) αcd11b bv650, by BD (1 mentions) αly6c percp cy5, by Thermo Fisher Scientific (1 mentions) αly6g af700, by Thermo Fisher Scientific (1 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions) Lsrii flow cytometer, by BD (1 mentions)

2 protocols using αcd8 fitc

Mouse MerTK-specific ASO for Immune Profiling

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A mouse MerTK-specific ASO and control ASOs were produced by Ionis Pharmaceuticals, Inc. Anti-mouse CTLA-4 (Catalog# BP0164) and anti-mouse PD-1 (Catalog# BE0146) were purchased from BioXCell. Liberase (Catalog #05401127001) and DNAse (Catalog #4716728001) were purchased from Roche and Sigma-Aldrich, respectively. Flow cytometry antibodies, including αCD45-PerCP Cy5.5 (Catalog# 103131), αCD4-PE/Dazzle594 (Catalog# 100456), αCD8-FITC (Catalog# 100706), αGranzyme B (GrB)-Pacific Blue (Catalog# 515408), αGr1-BV510 (Catalog# 108437), αCD11b-APC Fire750 (Catalog# 101262), αF4/80-Alexa Fluor 700 (Catalog# 123130), αCD38-PE-Cy7 (Catalog# 102718), αCD206-PE (Catalog# 141706), and αMertK-APC (Catalog# 151507) were ordered from BioLegend.

Hu Y., Revenko A., Barsoumian H., Bertolet G., Fowlkes N.W., Maazi H., Green M.M., He K., Sezen D., Voss T.A., Leyton C.S., Masrorpour F., Rafiq Z., Puebla-Osorio N., Leuschner C., MacLeod R., Cortez M.A, & Welsh J.W. (2024). Inhibition of MER proto-oncogene tyrosine kinase by an antisense oligonucleotide enhances treatment efficacy of immunoradiotherapy. Journal of Experimental & Clinical Cancer Research : CR, 43, 70.

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Characterization of Tumor-Infiltrating Immune Cells

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A/J mice were sacrificed 5 days after last treatment. Tumors and spleens were homogenized using a mouse Tumor Dissociation Kit (Miltenyi Biotec) according to manufacturer's instructions. Cell suspensions were stained according to manufacturer instructions. Intracellular staining for FoxP3 was performed using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience: 00-5523).
The stained cells were analyzed on an LSRII flow cytometer (BD Biosciences). Cells were gated and identified as follows: CD8 T-cells (CD8⁺), NK cells (CD8⁻, CD4⁻, CD49b⁺), T-regulatory cells (CD4⁺, CD25⁺, FoxP3⁺), monocytic MDSCs (CD45⁺, CD11b⁺, Ly6C^Hi), granulocytic MDSCs (CD45⁺, CD11b⁺, Ly6G^Hi), and dendritic cell (CD11b⁺, CD11c⁺, CD8⁺). Antibody conjugates were purchased from BioLegend: αCD8/FITC (100705), αCD4/PerCP-Cy5.5 (100539), αCD49b/PE/Cy7 (108921), αCD45.2/PE (109808), αCD11b/BV650 (101239), αPDL1/PE-Cy7 (124313), αPDL1/PE-Cy7 isotype (400617), αCD25/BV650 (102038), BD Pharmingen: αLy6C/PerCP/Cy5.5 (560525) and αLy6G/AF700 (561236), and eBioscience: FoxP3/AF700 (56-5773-80). Dead cells were excluded from analysis using Fixable Viability Dye eFluor 780 (eBioscience 65-0865-14).

Patel M.R., Jacobson B.A., Ji Y., Drees J., Tang S., Xiong K., Wang H., Prigge J.E., Dash A.S., Kratzke A.K., Mesev E., Etchison R., Federspiel M.J., Russell S.J, & Kratzke R.A. (2015). Vesicular stomatitis virus expressing interferon-β is oncolytic and promotes antitumor immune responses in a syngeneic murine model of non-small cell lung cancer. Oncotarget, 6(32), 33165-33177.

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