Clone mφp9

Manufactured by BD

Sourced in United States, Germany

The Clone MφP9 is a laboratory equipment designed for cell culture and research applications. It is a multi-functional platform that can be used for various cell-based experiments. The core function of the Clone MφP9 is to provide a controlled environment for cell growth and manipulation.

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Lab products found in correlation

Close spelling variants of this product

CD14-PerCP (clone MΦP9, (8 citations) Anti-CD14 (clone MΦP9) (3 citations) CD14-APC-Cy7™ (clone MφP9, (3 citations) Anti-CD14-APC-H7 (clone MφP9, (2 citations) CD14-PE (clone MφP9), (2 citations) Anti-CD14 FITC (Clone MφP9, (2 citations) CD14-Alexa Fluor 647 (clone MφP9; (2 citations) Mouse anti-human CD14 V450 (Clone MφP9, (2 citations)

4 protocols using clone mφp9

Cryopreserved Endometrial Cell Staining

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Fresh or cryopreserved single-cell suspensions from endometrial and decidual tissue, above, were stained with LIVE/DEAD Fixable Aqua Dead Cell Stain (Invitrogen, Cat# L34965) per manufacturer instructions. Cells were washed and treated with Human Fc Block (BD Biosciences, Cat# 564219, 1:200). For CD45+ cell purification for scRNAseq, cells were then washed and stained with CD45 (PerCP-Cy5.5, BioLegend, Clone 2D1, Cat# 368503, 1:100). All scRNAseq data presented, with the exception of Supplementary Figure 3, were generated from exclusively cryopreserved cells. For bulk NK (CD56+CD3-) and bulk macrophage (CD14+CD64+) cell purification for functional IOC experiments, cells were additionally stained with CD3 (BV785, BioLegend, Clone UCHT1, Cat# 300471, 1:100); CD14 (V450, BD Biosciences, Clone MφP9, Cat# 560349, 1:200); CD56 (PE, BD Biosciences, Clone B159, Cat# 555516, 1:50); and CD64 (PE-Cy7, BioLegend, Clone 10.1, Cat# 305022, 1:100). Antibody staining was performed at 4°C for 30 minutes in the dark. A BD FACSMelody sorter was used. All cells used for functional IOC experiments were isolated from cryopreserved single-cell suspensions. Cytospins were prepared in a cytocentrifuge at 600RPM for 3 minutes, after which cells were stained with hematoxylin and eosin.

Mani S., Garifallou J., Kim S.J., Simoni M.K., Huh D.D., Gordon S.M, & Mainigi M. (2024). Uterine macrophages and NK cells exhibit population and gene-level changes after implantation but maintain pro-invasive properties. Frontiers in Immunology, 15, 1364036.

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Multicolor Flow Cytometry of MSCs

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The surface antigen expression of MSCs was identified by flow cytometry using the following antibodies: CD14 (clone HCD14; BioLegend, San Diego, United States or clone MφP9; BD Biosciences, New Jersey, United States), CD34 (clone 8G12 also known as HPCA2), CD45 (clone HI30), CD73 (clone AD2), CD90 (clone 5E10), CD105 (clone 266), and HLA DRDPDQ (clone Tu39 also known as TÜ39) (all from BD Biosciences). The cells were stained as per manufacturer’s instructions (for staining details see Supplementary Table S1) and fluorescence intensities were measured using the FACSCelesta™ Cell Analyzer with BD FACSDiva™ software (BD Biosciences). Surface antigens were subdivided into identity markers (CD73, CD90, and CD105) and purity markers (CD14, CD34, CD45, and HLA DRDPDQ).

Jakl V., Ehmele M., Winkelmann M., Ehrenberg S., Eiseler T., Friemert B., Rojewski M.T, & Schrezenmeier H. (2023). A novel approach for large-scale manufacturing of small extracellular vesicles from bone marrow-derived mesenchymal stromal cells using a hollow fiber bioreactor. Frontiers in Bioengineering and Biotechnology, 11, 1107055.

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Monocyte Phagocytosis of MARV GP-Coated Beads

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MARV GP-coated beads were generated as described for ADNP. Antibodies were 5-fold serially diluted from 5 to 6.4 x 10⁻⁵ μg/mL in culture medium and incubated in triplicates with MARV GP-coated beads for 2 h at 37°C. Monocytes were enriched from human donor peripheral blood by negative selection (EasySep human monocyte enrichment kit; StemCell), and 5 x 10⁴ cells/well were added to the immune complexes for 4 h at 37°C. Cells were stained for CD14 (Clone MφP9, BD Biosciences) and CD16 (Clone 3G8, BD Biosciences), fixed with 4% paraformaldehyde, and analyzed by flow cytometry. A phagocytic score was determined as described for ADNP.

Ilinykh P.A., Huang K., Santos R.I., Gilchuk P., Gunn B.M., Karim M.M., Liang J., Fouch M.E., Davidson E., Parekh D.V., Kimble J.B., Pietzsch C.A., Meyer M., Kuzmina N.A., Zeitlin L., Saphire E.O., Alter G., Crowe JE J.r, & Bukreyev A. (2020). NON-NEUTRALIZING ANTIBODIES FROM A MARBURG INFECTION SURVIVOR MEDIATE PROTECTION BY FC-EFFECTOR FUNCTIONS AND ENHANCING EFFICACY OF OTHER ANTIBODIES. Cell host & microbe, 27(6), 976-991.e11.

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Multicolor Flow Cytometry for moDC and MΦ Phenotyping

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For surface staining moDC were incubated with FITC-conjugated mouse anti-CD40 mAB (10 µl/stain, Clone B-B20, Cat ab27281, abcam), APC-conjuagted mouse anti-CD83 mAB (10 µl/stain, Clone HB15e, Cat 551073, BD Pharmingen) and PE-conjugated mouse anti-CD14 mAB (10 µl/stain, Clone MΦP9, Cat 345785, BD Pharmingen) or isotype controls (5 µl/stain; mouse; monoclonal; FITC-IgG1, Cat ABIN118618, Antibodies-Online, Aachen, Germany; APC-IgG1, Cat 555751, BD; PE-IgG2b, Cat 555743, BD) for 15 min at 4°C and fixed in 1% PFA. MΦ were fixed and permeabilized using the Cytofix/Cytosperm kit (BD Pharmingen) and were then incubated with FITC-conjugated mouse anit-CD68 mAB (5 µl/stain, Clone Y1/82A, Cat 562117, BD Pharmingen), PE-conjugated mouse anit-CD163 mAB (10 µl/stain, Clone GHI/61, Cat 556018, BD Pharmingen), PerCP-conjugated mouse anti-HLA-DR mAB (10 µl/stain, Clone L243, Cat 347402, BD), and APC-conjugated mouse anti-CD206 mAB (5 µl/stain, Clone 19.2, Cat 550889, BD Pharmingen) or isotype controls (5 µl/stain; mouse; monoclonal; FITC-IgG2b, Cat 555057, BD; PE-IgG1, Cat 555749, BD; PerCP-IgG2a, Cat 349054, BD; APC-IgG1, Cat 555751, BD) for 30 min at 4°C. Flow cytometric analysis was performed on a FACSCanto II flow cytometer using the FACSDiva software v6.1.3 (BD Pharmingen). Data were analyzed using FlowJo software (Tree Star, Ashland, OR, USA).

Tudor C.S., Bruns H., Daniel C., Distel L.V., Hartmann A., Gerbitz A, & Buettner M.J. (2014). Macrophages and Dendritic Cells as Actors in the Immune Reaction of Classical Hodgkin Lymphoma. PLoS ONE, 9(12), e114345.

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