Colon tissues, cells, and EVs fractions were homogenized using the RIPA buffer (Jiangsu KeyGEN BioTECH, Nanjing, China), phosphatase inhibitor cocktail, and protease inhibitor cocktail (Jiangsu KeyGEN BioTECH, Nanjing, China). Pierce BCA Protein Assay Kit (Jiangsu KeyGEN BioTECH, Nanjing, China) was used to determine the protein concentration. Anti-CD63 (A5271, ABclonal), anti-Alix (12422-1-AP, Proteintech), anti-CD81 (66866-1-Ig, Proteintech), anti-Calnexin (ab22595, Abcam), anti-STING (19851-1-AP, Proteintech), anti-Phospho-STING (Ser366) (85735, Cell Signaling Technology), anti-Phospho-STING (Ser365) (72971, Cell Signaling Technology), anti-IRF3 (4302, Cell Signaling Technology), anti-Phospho-IRF3 (Ser396) (29047, Cell Signaling Technology), anti-NF-kB p65 (A19653, ABclonal), anti-Phospho-NF-kB p65 (Ser536) (3303, Cell Signaling Technology), anti-Beta-Actin (4970, Cell Signaling Technology), anti-GAPDH (AF1186, Beyotime Biotechnology) and secondary antibodies (KGP1201, Jiangsu KeyGEN BioTECH, Nanjing, China) were used for western blot.
Anti cd81 66866 1 ig
Manufactured by Proteintech
Sourced in China, United States
Anti-CD81 (66866-1-Ig) is a primary antibody that recognizes the CD81 protein. CD81 is a member of the tetraspanin family and plays a role in cell signaling and adhesion. This antibody can be used for various applications, such as Western blotting, immunohistochemistry, and flow cytometry, to detect and study the expression of CD81 in different cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Anti irf3, by Cell Signaling Technology (1 mentions)
Anti sting, by Proteintech (1 mentions)
Anti phospho nf kb p65 ser536, by Cell Signaling Technology (1 mentions)
Anti cd63, by ABclonal (1 mentions)
Anti alix 12422 1 ap, by Proteintech (1 mentions)
Anti phospho sting ser366, by Cell Signaling Technology (1 mentions)
Anti phospho sting ser365, by Cell Signaling Technology (1 mentions)
Anti nf kb p65, by ABclonal (1 mentions)
Anti phospho irf3 ser396, by Cell Signaling Technology (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Anti gapdh, by Beyotime (1 mentions)
Anti calnexin, by Abcam (1 mentions)
Protease inhibitor cocktail, by Keygen Biotech (1 mentions)
Pierce bca protein assay kit, by Keygen Biotech (1 mentions)
Phosphatase inhibitor cocktail, by Keygen Biotech (1 mentions)
Ripa buffer, by Keygen Biotech (1 mentions)
Anti tsg101 14497 1 ap, by Proteintech (1 mentions)
2 protocols using anti cd81 66866 1 ig
1
Proteomic Analysis of Colon Tissue Fractions
2
Exosomal Surface Marker Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To measure exosomal surface markers, western blot analysis was conducted. Briefly, the exosome supernatant was denatured in sodium dodecyl sulfonate (SDS) buffer and then incubated with anti-CD81 (66866-1-Ig; Proteintech, USA), anti-TSG101 (14497-1-AP; Proteintech), and anti-HSP70 (66183-1-Ig; Proteintech).
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