Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (DMEM/F-12) and antibiotic–antimycotic (penicillin G and streptomycin) solution were purchased from Gibco, Invitrogen, USA. Fetal bovine serum (FBS) was procured from Himedia, India. Hoechst 33342, propidium iodide (PI), Ribonuclease A (RNase A), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), acridine orange (AO), ethidium bromide (EtBr) and rhodamine 123 (Rh 123) were purchased from Himedia, India. Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit was supplied by BioVision, USA. The antibodies used in western blotting against β-actin, p53, Bax, Bcl-2, cleaved caspase-3, AKT, p-AKT473, mTOR, p-mTOR2448 were purchased from Cell Signaling Technology (Danvers, MA). Other chemicals were obtained from Sigma-Aldrich, USA and were of analytical grade.
Propidium iodide ribonuclease a rnase a 2 7 dichlorodihydrofluorescein diacetate dcfh da acridine orange
Manufactured by HiMedia
Sourced in India
Propidium iodide (PI) is a fluorescent dye that binds to DNA and is commonly used to assess cell viability and apoptosis. Ribonuclease A (RNase A) is an enzyme that catalyzes the hydrolysis of RNA. 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) is a cell-permeable fluorogenic dye used to detect reactive oxygen species. Acridine orange (AO) is a metachromatic dye that can be used to detect DNA and RNA in cells.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342, by HiMedia (2 mentions)
Rhodamine 123 rh 123, by HiMedia (2 mentions)
Ethidium bromide etbr, by HiMedia (2 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Dulbecco s modified eagle medium nutrient mixture f 12 dmem f 12, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin g, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by HiMedia (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Pakt473, by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
P mtor2448, by Cell Signaling Technology (1 mentions)
Annexin 5 fluorescein isothiocyanate fitc propidium iodide apoptosis detection kit, by Abcam (1 mentions)
Penicillin and streptomycin solution, by Merck Group (1 mentions)
2 protocols using propidium iodide ribonuclease a rnase a 2 7 dichlorodihydrofluorescein diacetate dcfh da acridine orange
1
DMEM/F-12 Media Cytotoxicity Assay
2
Cytotoxicity and Oxidative Stress Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (DMEM/F-12), fetal bovine serum (FBS), antibiotic penicillin and streptomycin solution and β-D-glucan powder were purchased from Sigma-Aldrich, USA. Hoechst 33342, propidium iodide (PI), Ribonuclease A (RNase A), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), acridine orange (AO), ethidium bromide (EtBr) and rhodamine 123 (Rh 123) were purchased from Himedia, India. All other chemicals and reagents used were of analytical grade.
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