CX6528, SGI1776, and lenalidomide were purchased from Selleckchem (Houston, TX). SGI1776 used for in vivo studies was provided by Tolero Pharmaceuticals, Inc (Lehi, Utah). Cycloheximide, bortezomib, and MG132 were purchased from Sigma-Aldrich (St. Louis, MO). Antibodies against PIM1, PIM2, PIM3 and ubiquitin were purchased from Abcam (Cambridge, MA). Antibodies against c-Myc, IRF4, pS6, and PARP were purchased from Cell Signaling (Danvers, MA). Annexin V and caspase 3 antibodies were purchased from BD Biosciences (San Jose, CA). IKZF1 antibody was purchased from Santa Cruz Biotechnology, Inc (Dallas, TX). IKZF3 antibody was purchased from Novus Biologicals LLC (Littleton, CO). Cereblon antibody was obtained from Sigma-Aldrich (St. Louis, MO).
Antibodies against c myc
Manufactured by Cell Signaling Technology
Sourced in United States
Antibodies against c-Myc are laboratory reagents used to detect and study the c-Myc protein, a transcription factor that plays a crucial role in cell growth and proliferation. These antibodies can be used in various techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to identify and quantify the expression of c-Myc in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Cycloheximide (chx), by Merck Group (2 mentions)
Cleaved caspase 3, by Cell Signaling Technology (2 mentions)
Ikzf1 antibody, by Santa Cruz Biotechnology (1 mentions)
Bortezomib, by Merck Group (1 mentions)
Lenalidomide, by Selleck Chemicals (1 mentions)
Sgi 1776, by Selleck Chemicals (1 mentions)
Mg132, by Merck Group (1 mentions)
Annexin 5, by BD (1 mentions)
Caspase 3, by BD (1 mentions)
Paclitaxel, by Solarbio (1 mentions)
Doxorubicin, by Solarbio (1 mentions)
4 hydroxytamoxifen, by Merck Group (1 mentions)
Cisplatin, by Solarbio (1 mentions)
Cellulose nitrate membrane, by Merck Group (1 mentions)
Protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
Mmp13, by Cell Signaling Technology (1 mentions)
Gm130, by Cell Signaling Technology (1 mentions)
Ripa lysis buffer, by Merck Group (1 mentions)
Mmp 2, by Cell Signaling Technology (1 mentions)
Mmp 9, by Cell Signaling Technology (1 mentions)
8 protocols using antibodies against c myc
1
Evaluating PIM and IKZF Inhibitors in MM
2
Breast Cancer Cell Line Profiling
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4-Hydroxytamoxifen (TAM) was purchased from Sigma-Aldrich; Merck KGaA. Doxorubicin, paclitaxel and cisplatin were purchased from Beijing Solarbio Science & Technology Co., Ltd. Antibodies against Erα (cat. no. 8644S), human epidermal growth factor receptor 2 (HER2) (cat. no. 2242S), GAPDH (cat. no. 5174S) and AKT (cat. no. 9272S) were purchased from Cell Signaling Technology, Inc. Antibodies against c-MYC (cat. no. ab32072), cyclin D1 (cat. no. ab226977, phospho (p)-AKT (Ser473) (cat. no. ab81283) and E-cadherin (cat. no. ab133597) were purchased from Abcam. Antibodies against p21 (cat. no. 27296-1-AP) and β-catenin (cat. no. 51067-2-AP) were purchased from ProteinTech Group, Inc., and an antibody against vimentin (cat. no. BS1491) was purchased from Bioworld Technology, Inc.
3
Protein Expression Analysis in Cells
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Cells were lysed using RIPA lysis buffer (Millipore, Billerica, MA, USA) with a protease and phosphatase inhibitor cocktail (Thermo Fisher). Samples were subjected to immunoblotting as previously described28 (link). The transfer process was performed using a cellulose nitrate membrane (0.22 μm, Millipore) under constant current conditions of 200 mA for 2 h. Antibodies against GP73 were purchased from Thermo Fisher (1:3000); antibodies against c-Myc, HIF-1α, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13 and GM130 were purchased from Cell Signaling Technology, Danvers, MA, USA (1:2000); antibodies against FLAG-tag and β-actin were purchased from EarthOX, San Francisco, CA, USA (1:5000).
4
Cisplatin-Induced Apoptosis Signaling Pathway
5
Biochemical Protein Interaction Analysis
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All chemicals were purchased from Sigma-Aldrich unless noted otherwise. Antibodies against c-myc, cleaved caspase3, cleaved PARP, and GST were purchased from Cell Signaling Technology (Beverly, MA). Anti-GAPDH, γ-H2A.X, β-actin, CDK5, p35, GFP, HA, p-S/T/Y, and Thiophosphate ester antibodies were from Abcam (Cambridge, MA). Anti-CLIC4 antibody was from Novagen (Madison, WI). Fluorescent anti-mouse or anti-rabbit IgG antibodies conjugated with Alexa Fluro 488 or Alexa Fluro 594 were from Invitrogen. Protein G Dynabeads were from Life technologies, and Glutathione Sepharose beads were from GE. ATP-γ-S and p-nitrobenzyl mesylate (PNBM) were from Abcam.
6
Investigating Cellular Stress Pathways
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Tunicamycin (TM), thapsigargin (TG), lysosome inhibitor E64d, and proteasome inhibitor MG-132 were purchased from Calbiochem (Billerica, MA). Cycloheximide was obtained from Sigma (St. Louis, MO). Antibodies against c-Myc, AT, GRP78, p-IRE1, p-PERK, and ATF6 were obtained from Cell Signaling Technology (Boston, MA). ELISA Kits for detecting AT were purchased from R&D Systems (Minneapolis, MN). HRP-conjugated anti-mouse and anti-rabbit secondary antibodies were purchased from Jackson ImmunoResearch (West Grove, PA).
7
Cryptolepine Hydrate Modulates Mitochondrial Dynamics
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Purified cryptolepine hydrate (≥98% purity by HPLC), MTT dye, 2′,7′-dichlorofluorescin diacetate, and Rhodamine 123 were purchased from Sigma-Aldrich (St. Louis, MO). The MitoBiogenesis In-cell ELISA kit was purchased from Abcam (Cambridge, MA). Antibodies against c-Myc, p-Drp1, LKB1, and AMPKα1/2 were purchased from Cell Signaling Technology (Beverly MA). The Opa1 antibody was obtained from Thermo Scientific (Rockford, IL). Antibodies against PGC-1α, Mitofusin 1, Mitofusin 2, Drp1, p-AMPKα1/2, SIRT1, and β-actin and horseradish peroxidase (HRP)-labeled anti-mouse and anti-rabbit were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). The ATP Determination kit, MitoTracker Red CMXRos, AlexaFluore 488- and 594-conjugated secondary antibodies were obtained from Molecular Probes (Eugene, OR).
8
Proteomic Analysis of Glycolytic Enzymes
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