Protein molecular weight standards

E. coli DH5α and E. coli Rosetta (DE3) were stored at an ultra-low temperature freezer for preservation. Full-length gene encoding UmIDH was synthesized by Generay Biotech Co., Ltd. (Shanghai, China), and the coding sequence was codon optimized according to the Escherichia coli bias. The plasmid used was pET28b, and the restriction enzyme sites at both ends of the gene were NdeI and XhoI. PrimeSTAR Max DNA Polymerase was purchased from TaKaRa (Dalin, China). Restriction enzymes (NdeI, XhoI, and DpnI), T4 DNA ligase, and Protein Molecular Weight Standards were purchased from Thermo Fisher Scientific (Shanghai, China). The TALON Metal Affinity Resin used for protein purification was obtained from TaKaRa. All other biochemical reagents were sourced from Sangon Biotech and BBI (Shanghai, China).

E. coli DH5α cells, E. coli Rosetta (DE3) cells, and the pET-28b(+) expression vector were preserved at low temperature in our laboratory. PrimeSTAR Max DNA Polymerase was purchased from TaKaRa (Dalin, China). Restriction enzymes (NdeI and XhoI), T4 DNA ligase, and Protein Molecular Weight Standards were purchased from Thermo Fisher Scientific (Shanghai, China). The TALON Metal Affinity Resin used for protein purification was obtained from TaKaRa. The DL-isocitrate and coenzymes (NAD⁺ and NADP⁺) were purchased from Sigma–Aldrich (Shanghai, China). All other biochemical reagents were obtained from Sangon Biotech and BBI (Shanghai, China).