L 755 507

Manufactured by Bio-Techne

L-755,507 is a synthetic compound that functions as an inhibitor. It is primarily used for research purposes in laboratory settings.

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Lab products found in correlation

3 isobutyl 1 methylxanthine ibmx, by Merck Group (1 mentions) Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions) Forskolin, by Merck Group (1 mentions) Isoproterenol, by Merck Group (1 mentions) Ici 118 551, by Bio-Techne (1 mentions) Carvedilol, by Tokyo Chemical Industry (1 mentions) Nebivolol, by Bio-Techne (1 mentions) Atenolol, by Bio-Techne (1 mentions) L748337, by Bio-Techne (1 mentions) Aicar, by Merck Group (1 mentions) D12345, by Thermo Fisher Scientific (1 mentions) Trichostatin a, by Merck Group (1 mentions) Resveratrol, by Selleck Chemicals (1 mentions) Mln4924, by Adooq (1 mentions) Veliparib abt 888, by Selleck Chemicals (1 mentions) Rucaparib ag 014699 pf 01367338, by Selleck Chemicals (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)

4 protocols using l 755 507

Receptor Binding Assay Protocol

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Carvedilol was purchased from TCI America (Portland, OR). 4-(3-t-butylamino-2-hydroxypropoxy)-[5,7-3H]benzimidazole-2-one (3H-CGP) with a specific activity of 41.7 Ci/mmol was purchased from Perkin Elmer (Waltham, MA). Isoproterenol, 3-isobutyl-1-methylxanthine (IBMX), and forskolin were purchased from Sigma (St. Louis, MO). Atenolol, nebivolol, ICI-118,551, L-748,337, xamoterol humifumerate, formoterol humifumerate and L-755,507 were obtained from Tocris (Bristol, United Kindom). EGF was purchased from Peprotech (Rocky Hill, NJ) and dissolved in sterile deionized water as 100 ug/mL stock and stored in −20°C freezer.

Chang A., Yeung S., Thakkar A., Huang K.M., Liu M.M., Kanassatega R.S., Parsa C., Orlando R., Jackson E.K., Andresen B.T, & Huang Y. (2014). Prevention of skin carcinogenesis by the β-blocker carvedilol. Cancer prevention research (Philadelphia, Pa.), 8(1), 27-36.

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Small Molecule Compound Preparation

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Commercially available small molecules used in this study were NU7026 (SIGMA, T8552), Trichostatin A (SIGMA, T8552), MLN4924 (Adooq BioScience, A11260), NSC 19630 (Calbiochem, 681647), NSC 15520 (ChemBridge, 6048069), AICAR (SIGMA, A9978), RS-1 (Calbiochem, 553510), Resveratrol (Selleckchem, S1396), SCR7 (XcessBio, M60082-2s), L755507 (TOCRIS, 2197), B02 (SIGMA, SML0364), and STL127685 (Vitas-M). STL127685 is a 4-fluorophenyl analog of the non-commercially available STL127705. Stocks of 15 mM (or 10 mM for NU7026) were made using dimethylsulfoxide (DMSO) (Thermo Scientific, D12345). Solubility is a limiting factor for NU7026 concentration. Suitable working solutions for different concentrations were made so that addition of each small molecule accounts for a final concentration of 0.08% (or 0.2% for NU7026) DMSO in the media. Addition of all small molecules would lead to a final concentration of 0.7% DMSO.

Riesenberg S, & Maricic T. (2018). Targeting repair pathways with small molecules increases precise genome editing in pluripotent stem cells. Nature Communications, 9, 2164.

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NHEJ Inhibitors to Enhance HDR

Cited 1 time

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The following NHEJ inhibitors were used to enhance HDR: vanillin (12 (link)) reconstituted in H₂O, 300µM final concentration (Sigma cat#V1104); SCR7-X in DMSO, 1µM final (Xcess Biosciences cat#M60082). Since we purchased SCR7-X from Xcess Biosciences we refer to this compound as “SCR7-X” as recently suggested (13 (link)). Rucaparib/AG-014699/PF-01367338, in DMSO, 1µM final (Selleckchem cat#S1098); veliparib/ABT-888 in DMSO, 5µM final (Selleckchem cat#S1004); RS-1 (14 ) in DMSO, 7.5µM final (MerckMillipore cat# 553510); RS-1 in DMSO, 7.5µM final, (Sigma cat#R9782); Luminespib/AUY-922/NVP-AUY922 in DMSO, 1µM final (Selleckchem cat#S1069); L-755,507 in DMSO, 5µM final (Tocris cat#2197); vanillin derivatives (12 (link)) 6-nitroveratraldehyde in DMSO, 3µM final (Maybridge cat#11427047), 4,5-dimethoxy-3-iodobenzaldehyde in DMSO, 3µM final (Maybridge cat#11328426); 6-bromoveratraldehyde in DMSO, 3µM final (Maybridge cat#11480124).

Kornete M., Marone R, & Jeker L.T. (2018). Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary T Cells. The Journal of Immunology Author Choice, 200(7), 2489-2501.

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Culturing HEK-293A and U2OS cell lines

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HEK-293A human embryonic kidney and U2OS osteosarcoma cell lines (parental U2OS and U2OS^Clover–PML) were cultured in Dulbecco's modified Eagle's medium (Life Technologies) supplemented with 10% fetal calf serum, at 37°C with 5% CO₂. U2OS cell lines stably expressing GFP-PML-I and GFP-PML-IV (29 (link)) were maintained as above with the addition of 800 (PML-I) or 1600 (PML-IV) μg/ml G418. The RAD51-stimulatory compound RS-1 (Sigma), the DNA ligase IV inhibitor SCR7 (1 μM, XcessBio) and HDR stimulatory compound L755507 (5 μM, Tocris) were added to culture media from 10 mg/ml stock solutions in DMSO.

Pinder J., Salsman J, & Dellaire G. (2015). Nuclear domain ‘knock-in’ screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing. Nucleic Acids Research, 43(19), 9379-9392.

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