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Bulge loop mir rt qpcr starter kit

Manufactured by RiboBio
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The Bulge-Loop miR RT-qPCR Starter kit is a laboratory equipment product designed for the detection and quantification of microRNA (miRNA) expression levels. The kit includes reagents and protocols for reverse transcription (RT) and quantitative real-time PCR (qPCR) analysis of miRNA targets.

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Lab products found in correlation

M mlv first strand kit, by Thermo Fisher Scientific (3 mentions) Sybr green, by Solarbio (3 mentions) Rnasimple total rna kit, by Tiangen Biotech (2 mentions) Rnasimple total kit, by Tiangen Biotech (1 mentions)

3 protocols using bulge loop mir rt qpcr starter kit

1

Quantifying circVPS33B, VPS33B, and HNRNPK

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RNA Simple Total Kit (TIANGEN, Beijing, China) was utilized for total RNA separation (tissue samples and cells). The complementary DNA was synthesized with the M-MLV First Strand Kit (Life Technologies) or Bulge-Loop miR RT-qPCR Starter kit (RiboBio), followed by performing qPCR with the SYBR Green (Solarbio). Primer sequences used in the study were as follows: circVPS33B (Forward: 5ʹ-GTGGTGTTCTTGGGTGGTTGT-3ʹ; Reverse: 5ʹ-CCGCTCTAGCACCTTTCTCTC-3ʹ), VPS33B (Forward: 5ʹ-ATGAGCCCTTTGGATCGAATTG-3ʹ; Reverse: 5ʹ-ATGCGGGGTCTGACCAAGA-3ʹ), HNRNPK (Forward: 5ʹ-CAATGGTGAATTTGGTAAACGCC-3ʹ; Reverse: 5ʹ-GTAGTCTGTACGGAGAGCCTTA-3ʹ), β-actin (Forward: 5ʹ-CTCGCCTTTGCCGATCC-3ʹ; Reverse: 5ʹ-TCTCCATGTCGTCCCAGTTG-3ʹ), miR-873-5p (Forward: 5ʹ-GGGGCAGGAACTTGTGAG-3ʹ; Reverse: 5ʹ-GTGTGGTGTGGTATGGTGTG-3ʹ), and U6 small nuclear RNA (U6) (Forward: 5ʹ-CGCTTCGGCAGCACATATACTAAAATTGGAAC-3ʹ; Reverse: 5ʹ-GCTTCACGAATTTGCGTGTCATCCTTGC-3ʹ). Relative expression of circVPS33B, VPS33B mRNA, HNRNPK mRNA, and miR-873-5p was evaluated by 2−ΔΔCt method, and β-actin or U6 was used as an internal reference.
Lu Y., Cheng J., Cai W., Zhuo H., Wu G, & Cai J. (2021). Inhibition of circRNA circVPS33B Reduces Warburg Effect and Tumor Growth Through Regulating the miR-873-5p/HNRNPK Axis in Infiltrative Gastric Cancer. OncoTargets and therapy, 14, 3095-3108.
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2

Quantifying circVPS33B and miR-873-5p Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNAsimple Total RNA Kit from TIANGEN (Beijing, China) was utilized for total RNA separation (tissue samples and cells). The complementary DNA was synthesized with the M-MLV First Strand Kit (Life Technologies) or Bulge-Loop miR RT-qPCR Starter kit (RiboBio) and then used for qPCR with the SYBR Green (Solarbio). The primers were used as follows: circVPS33B (Forward: 5'-GTGGTGTTCTTGGGTGGTTGT-3'; Reverse: 5'-CCGCTCTAGCACCTTTCTCTC-3'), VPS33B (Forward: 5'-ATGAGCCCTTTGGATCGAATTG-3'; Reverse: 5'-ATGCGGGGTCTGACCAAGA-3'), HNRNPK (Forward: 5'-CAATGGTGAATTTGGTAAACGCC-3'; Reverse: 5'-GTAGTCTGTACGGAGAGCCTTA-3'), β-actin (Forward: 5'-GCACCACACCTTCTACAATG-3'; Reverse: 5'-TGCTTGCTGATCCACATCTG-3'), miR-873-5p (Forward: 5'-CGCATGGCAGTGGTTTTACCCTA-3'; Reverse: 5'-ATCCAGTGCAGGGTCCGAGG-3'), and U6 small nuclear RNA (U6) (Forward: 5'-CGCTTCGGCAGCACATATACTAAAATTGGAAC-3'; Reverse: 5'-GCTTCACGAATTTGCGTGTCATCCTTGC-3'). The levels of circVPS33B, VPS33B, HNRNPK, and miR-873-5p were evaluated by 2 -ΔΔCt method, and β-actin or U6 was used as an internal.
Lu Y., Cheng J., Cai W., Zhuo H., Wu G., & Cai J. (2020). Inhibition of circRNA circVPS33B reduces cell malignant behaviors and Warburg effect through regulation of the miR-873-5p/HNRNPK axis in infiltrative gastric cancer.
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3

Quantifying circVPS33B and miR-873-5p Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNAsimple Total RNA Kit from TIANGEN (Beijing, China) was utilized for total RNA separation (tissue samples and cells). The complementary DNA was synthesized with the M-MLV First Strand Kit (Life Technologies) or Bulge-Loop miR RT-qPCR Starter kit (RiboBio) and then used for qPCR with the SYBR Green (Solarbio). The primers were used as follows: circVPS33B (Forward: 5'-GTGGTGTTCTTGGGTGGTTGT-3'; Reverse: 5'-CCGCTCTAGCACCTTTCTCTC-3'), VPS33B (Forward: 5'-ATGAGCCCTTTGGATCGAATTG-3'; Reverse: 5'-ATGCGGGGTCTGACCAAGA-3'), HNRNPK (Forward: 5'-CAATGGTGAATTTGGTAAACGCC-3'; Reverse: 5'-GTAGTCTGTACGGAGAGCCTTA-3'), β-actin (Forward: 5'-GCACCACACCTTCTACAATG-3'; Reverse: 5'-TGCTTGCTGATCCACATCTG-3'), miR-873-5p (Forward: 5'-CGCATGGCAGTGGTTTTACCCTA-3'; Reverse: 5'-ATCCAGTGCAGGGTCCGAGG-3'), and U6 small nuclear RNA (U6) (Forward: 5'-CGCTTCGGCAGCACATATACTAAAATTGGAAC-3'; Reverse: 5'-GCTTCACGAATTTGCGTGTCATCCTTGC-3'). The levels of circVPS33B, VPS33B, HNRNPK, and miR-873-5p were evaluated by 2 -ΔΔCt method, and β-actin or U6 was used as an internal.
Lu Y., Cheng J., Cai W., Zhuo H., Wu G., & Cai J. (2020). Inhibition of circRNA circVPS33B reduces cell malignant behaviors and Warburg effect through regulation of the miR-873-5p/HNRNPK axis in infiltrative gastric cancer .
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