Imidazole

Standard chemicals were purchased from Sigma Aldrich and sodium acetate was purchased from Scharlau, imidazole was purchased from PanReac AppliChem and IPTG was purchased from Fischer Bioreagents. Enzymes for standard cloning procedures were purchased from Thermo Fisher Scientific and New England Biolabs, respectively.

For protein expression and purification, pET3a plasmids encoding c-terminal histidine-tagged ColN was provided by Professor Jeremy H. Lakey, Newcastle University, UK. Luria-Bertani broth and agar were bought from Hardy Diagnostics (Santa Maria, CA, USA). L-(+)-arabinose was obtained from TCI (Tokyo, Japan). Imidazole, DNase I, and ampicillin sodium salt were purchased from PanReac Applichem (Darmstadt, DE, USA). PierceTM protease inhibitor tablets and bicinchoninic acid (BCA) protein assay kit was purchased from Thermo Scientific (Waltham, MA, USA). 10X Phosphate Buffered Saline (PBS), skim milk powder and sodium chloride (NaCl) were purchased from Vivantis Technologies (Selangor, MY) and Ajax Finechem (Seven Hills, NSW 2147, Australia).
For cell culture, DMEM/Ham’s F-12, DMEM high glucose and 1X HEPES were bought from HyClone (Logan, Utah), 10% fetal bovine serum (FBS) from Merck (Darmstadt, Germany), 1% penicilin/streptomycin from Gibco (Gaithersburg, MA, USA). The reagents 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Abcam (Cambridge, CB2 0AX, UK). Cisplatin and Doxorubicin were bought from Sigma Chemical, Inc. (St. Louis, MO, USA).