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Pmal c vector

Manufactured by New England Biolabs
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The PMAL-c vector is a cloning and expression vector designed for the production of recombinant proteins in Escherichia coli. It features a strong tac promoter for high-level expression and a maltose-binding protein (MBP) fusion tag to enhance solubility and facilitate purification of the target protein.

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Lab products found in correlation

Pgex 4t 2 vector, by GE Healthcare (1 mentions)

Close spelling variants of this product

PMAL vector PMAL-c

2 protocols using pmal c vector

1

Kinase Domain Expression and Phosphorylation Assay

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The cDNAs corresponding to SnRK1α1, SnRK1β1, SnRK1β2, SnRK1β3, SnRK1βγ, and SnAK2 were expressed as described by Maya‐Bernal et al. (2017 (link)). The kinase domain (KD) from SnRK1α1, SnRK1α2 and the activated kinase SnAK2 were cloned into the pGEX4T2 vector (GE Healthcare). For the γ‐32P incorporation assays, the KD from SnRK1α1 and SnRK1α2 were cloned into pMAL‐c vector (NEB). Full‐length PHR1 (At4G28610) and the nonphosphorylated mutant S11A were cloned into the pET28b(+) vector (Novagen). Point mutations were introduced according to the protocol described by Heckman and Pease (2007 (link)).
Trejo‐Fregoso R., Rodríguez I., Ávila A., Juárez‐Díaz J.A., Rodríguez‐Sotres R., Martínez‐Barajas E, & Coello P. (2022). Phosphorylation of S11 in PHR1 negatively controls its transcriptional activity. Physiologia Plantarum, 174(6), e13831.
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2

Anti-GABAA Receptor β3 Subunit Antibody

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The rabbit anti-GABAAR β3 subunit antiserum (anti-GABAAR β3) was raised as described previously (Todd et al., 1996 (link)). For the sub-cloning of fusion protein consisting of maltose-binding protein (MBP) and amino acids 345–408 of the mouse GABAAR β3, the DNA sequence corresponding to amino acid 345–408 of the GABAAR β3 was amplified by PCR, using FANTOM3 clone C630014N19 (RIKEN Genomic Sciences Research Complex) as a template (Carninci et al., 2005 (link)), and sub-cloned into pMAL-C vector (New England Biolabs).
Bannai H., Niwa F., Sherwood M.W., Shrivastava A.N., Arizono M., Miyamoto A., Sugiura K., Lévi S., Triller A, & Mikoshiba K. (2015). Bidirectional Control of Synaptic GABAAR Clustering by Glutamate and Calcium. Cell Reports, 13(12), 2768-2780.
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