Doxorubicin (Solarbio, Beijing, China), formaldehyde (Solarbio, Beijing, China), heparin sodium (Solarbio, Beijing, China), acetonitrile (Merck, Germany), formic acid (CNW, Germany), BCA Kit (Solarbio, Shangahi, China), Tris (Sigma, USA), SDS (Bio-Rad, USA), phenylmethylsulfonyl fluoride (Solarbio, Beijing), phosphatase inhibitor (Solarbio, Beijing, China), RIPA buffer (Solarbio, Beijing, China), PTP1B (Abcam, USA), IRS-1, P-IRS, HK2, HIF-1α (Cell Signaling Technology, Inc., USA), Nrf2 (Abcam, USA), NH4HCO3 (Sigma, USA), High pH Reversed-Phase Peptide Fractionation Kit (Pierce, USA), TMT 6/10 plex Isobaric Label Reagent (Thermo, USA) were used.
Tmt 6 10 plex isobaric label reagent
Manufactured by Thermo Fisher Scientific
Sourced in United States
The TMT 6/10 plex Isobaric Label Reagent is a set of labeling reagents used in mass spectrometry for the quantitative analysis of proteins across multiple samples. The reagents are designed to enable simultaneous identification and relative quantitation of peptides and proteins in complex biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Doxorubicin, by Solarbio (1 mentions)
Phosphatase inhibitor, by Solarbio (1 mentions)
Sodium dodecyl sulfate (sds), by Bio-Rad (1 mentions)
P irs, by Cell Signaling Technology (1 mentions)
Formaldehyde, by Solarbio (1 mentions)
Ptp1b, by Abcam (1 mentions)
Irs 1, by Cell Signaling Technology (1 mentions)
Hif 1α, by Cell Signaling Technology (1 mentions)
Nh4hco3, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Bca kit, by Solarbio (1 mentions)
Ripa buffer, by Solarbio (1 mentions)
Heparin sodium, by Solarbio (1 mentions)
Phenylmethylsulfonyl fluoride, by Solarbio (1 mentions)
Peptide desalting spin column, by Thermo Fisher Scientific (1 mentions)
Trypsin gold, by Promega (1 mentions)
Ripa lysis buffer, by Keygen Biotech (1 mentions)
2 protocols using tmt 6 10 plex isobaric label reagent
1
Quantitative Proteomic Analysis of Doxorubicin-Induced Cellular Changes
2
Protein Extraction and TMT Labeling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein extraction and peptide digestion were performed as described previously 18 (link). Briefly, lung tissues were lysed in RIPA lysis buffer (Keygen Biotech), and the proteins in lysates were concentrated and digested with Trypsin Gold (Promega) for 16 h at 37°C. The digested peptides were desalted with C18 cartridge and dried by vacuum. The peptides were then labeled with TMT6/10plex™ Isobaric Label Reagent (Thermofisher) following the manufacturer's instructions. The labeled peptides were desalted using peptide desalting spin columns (ThermoFisher, 89852).
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