α glucosidase from saccharomyces

Acetic acid, ethanol 96%, ethyl acetate, methanol, thymol, sulfuric acid 95%, sodium acetate buffer, and phosphate buffer were from P.O.Ch. (Gliwice, Poland). Acetylcholinesterase enzyme (AChE) from Electrophorus electricus, p-anisaldehyde, bovine serum albumin (BSA), 1,1-diphenyl-2-picrylhydrazyl (DPPH), L-DOPA, Fast Blue B Salt, lipase from Porcine pancreas, 1-naphthyl acetate, 2-naphthyl acetate, 2-naphthyl α-D glucopyranoside, α-glucosidase from Saccharomyces, Natural Product—NP reagent (diphenylboryloxyethylamine), polyethylene glycol-4000, tyrosinase from mushroom and TRIS hydrochloride were purchased from Sigma Aldrich (Poznań, Poland). The MTT dye (3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyltetrazolium bromide), HEPES buffer, and Triton X-100 were from Sigma Aldrich (Poznań, Poland). Agarose, Mueller-Hinton (M-H) agar, and M-H broth were purchased from Biocorp (Warsaw, Poland). The antibacterial activity was tested toward reference Gram-positive bacteria: Bacillus subtilis (ATCC 6633) was purchased from the American Type Culture Collections.
The standards of lignans were supplied by Chem-Faces Biochemical (Wuhan, China). The lignans working standards solutions were prepared in methanol.

α-glucosidase from Saccharomyces cerevisiae, 4-nitrophenyl α-D-glucopyranoside (pNPG), lipase from porcine pancreas, 4-nitrophenyl butyrate (NPB), Nile Red, fructose, acarbose, and aminoguanidine bicarbonate (AMG) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Na₂HPO₄, NaH₂PO₄, Tris–HCl, CaCl₂, KH₂PO₄, and Na₂N₃ were purchased from Panreac Quimica (Castellar del Vallès, Barcelona, Spain). Bovine serum albumin (BSA) was purchased from Santa Cruz Biotechnology, Inc., and orlistat from Acofar (Terrassa, BCN, Spain). Ethanol, mEthanol, and formic acid were purchased from Carlo Erba Reagents (Val de Reuil Cedex, France).