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39 protocols using accu chek active glucometer

1

Pyruvate Tolerance Test Protocol

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The animals were fasted overnight, and then, pyruvate (Sigma) was administered intraperitoneally (2 g/kg body weight) between 9am and 10am. Blood samples were collected from the cut tip of the tail at 0, 15, 30, 60, 90 and 120 min. The blood glucose concentration was measured using Accu-Chek Active glucometer (Roche Diagnostic, Rotkreuze, Switzerland). The glucose response during the pyruvate tolerance test was calculated by estimating the total area under the glucose curve11 (link).
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2

Metformin's Impact on Murine Metabolism

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To determine whether the application of Met affects body weight and blood glucose levels in mice, we measured the body weight and random blood glucose levels of the animals once a week, as previously described [29 ]. After blood was collected from the caudal vein, an ACCU-CHEK Active Glucometer (Roche Diagnostic, Mannheim, Germany) was used to evaluate the blood glucose levels of each group.
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3

Biochemical Markers in Fasting Subjects

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The concentrations of cholesterol, triglyceride, serum creatinine, and blood urea nitrogen were measured using an automatic biochemical analyzer (Dade Behring Marburg GmbH, Germany). Fasting blood glucose (FBG) was measured by Accu-Chek Active glucometer (Roche).
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4

Glucose and Insulin Tolerance in Mice

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For glucose tolerance tests, mice were fasted for 6 h and challenged with an intraperitoneally (i.p.) injection of glucose (2 g/kg). For insulin tolerance tests, mice were fasted for 6 hr and given an i.p. injection of insulin (1 U/kg). Blood glucose levels were monitored using the ACCUCHEK active glucometer (Roche) at times indicated in figure legends.
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5

Measuring Plasma Glucose in Mice

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Mouse blood was drawn from the tail vein and plasma glucose concentration was measured with ACCU-CHEK Active Glucometer (Roche Diagnostics, Mannheim, Germany) as reported20 (link).
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6

Plasma Metabolite Profiling in Mice

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Blood was collected by puncture of the v.facialis from ad libitum fed or 12 h-fasted mice. TG, TC (DiaSys, Holzheim, Germany), and β-hydroxybutyrate (Cayman, Ann Harbor, MI) concentrations were measured enzymatically according to manufacturer's instructions. Lipoprotein fractions from 200 μl pooled plasma per genotype were separated using fast protein liquid chromatography (Pharmacia P-500) equipped with a Superose 6 column (Amersham Biosciences, Piscataway, NJ) and TG and TC concentrations were assayed spectrophotometrically. Alanine aminotransferase and aspartate aminotransferase levels were measured enzymatically (Roche Diagnostics, Risch, Switzerland). Blood glucose concentration was determined using Accu-Chek® Active glucometer and glucose strips (Roche Diagnostics).
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7

Insulin-Induced Glycemic Response in Mice

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Four hour-fasted mice were i.p. injected with 1 IU insulin/kg body weight in PBS (100 IU/ml stock; Actrapid Novo Nordisk, Vienna, Austria). Blood glucose levels were measured 0, 15, 30, 45, 60, and 120 min post-injection using Accu-Chek® Active glucometer and glucose strips (Roche Diagnostics).
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8

Glycemic Index Evaluation of Dishes

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Measurements of blood glucose concentration obtained, respectively, after drinking the reference solution and consuming the analysed dishes were performed in the morning hours after 10-h night fasting. Before consuming the analysed product, blood was sampled from the participants using an automatic Accu–Chek Softclix lancing device (Roche Diagnostics, Rotkreuz, Switzerland) from a fingertip puncture. Further blood samples were taken at 15, 30, 45, 60, 90 and 120 min, after starting the consumption of the reference solution or a tested dish. Following each blood drop sampling, the blood glucose concentration was measured using an Accu-Chek Active glucometer (Roche Diagnostics). Results were recorded in measuring sheets. The participants had 5–10 min to drink the reference solution and 10–15 min to eat the analysed dishes. Glycaemic curves were plotted twice after drinking the glucose solution and once after consuming the analysed dish. Each participant took part in the determination of the GI of at least one dish, made according to the traditional and modified recipes, respectively. Steamed dumplings and rolls with cheese were consumed by 14 participants, whereas waffles and apple pancakes were consumed by 11 participants.
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9

Glucose Tolerance Test in Mice

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Six hour-fasted mice were administered i.p. with 2 g glucose/kg body weight in PBS. Blood glucose levels were measured 0, 15, 30, 60, and 120 min after injection using Accu-Chek® Active glucometer and glucose strips (Roche Diagnostics).
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10

Insulin-Induced Glycemic Response in Mice

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Four hour-fasted mice were i.p. injected with 1 IU insulin/kg body weight in PBS (100 IU/ml stock; Actrapid Novo Nordisk, Vienna, Austria). Blood glucose levels were measured 0, 15, 30, 45, 60, and 120 min post-injection using Accu-Chek® Active glucometer and glucose strips (Roche Diagnostics).
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