Evaluations of device position after two weeks post-surgery were performed by MicroCT and MRI analysis. For MicroCT, see the procedures described previously. MRI was performed on a 9.4 T Bruker Biospec MRI system with a 30 cm bore, a 12 cm gradient insert, and an Autopac automated sample positioning system (Bruker Biospin, Billerica, MA). Respiratory signals were recorded using an MR-compatible physiologic monitoring system (SA Instruments, NY, USA). A warm water circulating system maintained body temperature. An actively decoupled, 4-channel phased array, receive-only radiofrequency coil designed specifically for the mouse brain (Bruker Biospin, MA, USA) was mounted on the bed. This assembly was centered inside a 72 mm quadrature volume coil in transmit-only mode (Bruker Biospin, Inc, MA, USA). Mice were imaged using an accelerated spin-echo sequence (Turbo Rapid Acquisition with Relaxation Enhancement, TurboRARE) oriented in axial, sagittal, and coronal directions. The following parameters were used: TR/TE = 1250 ms/21.3 ms, RARE factor 8, MTX = 256 x 256, FOV 3 x 3 cm, 7-13 slices of 0.75-1 mm thick (as needed for full brain coverage), flip back enabled, and 3 signal averages. The acquisition time was approximately 2 min per scan.
72 mm quadrature volume coil
Manufactured by Bruker
Sourced in United States
The 72 mm quadrature volume coil is a specialized lab equipment designed for magnetic resonance imaging (MRI) applications. It serves as a radiofrequency (RF) transmit and receive coil, enabling the generation and detection of the necessary electromagnetic fields for MRI data acquisition. The coil operates in a quadrature configuration, which provides improved signal-to-noise ratio and uniformity of the RF field.
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2 protocols using 72 mm quadrature volume coil
1
In-Vivo Imaging of Surgical Implants
2
In-Vivo Imaging of Surgical Implants
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Evaluations of device position after two weeks post-surgery were performed by MicroCT and MRI analysis. For MicroCT, see the procedures described previously. MRI was performed on a 9.4 T Bruker Biospec MRI system with a 30 cm bore, a 12 cm gradient insert, and an Autopac automated sample positioning system (Bruker Biospin, Billerica, MA). Respiratory signals were recorded using an MR-compatible physiologic monitoring system (SA Instruments, NY, USA). A warm water circulating system maintained body temperature. An actively decoupled, 4-channel phased array, receive-only radiofrequency coil designed specifically for the mouse brain (Bruker Biospin, MA, USA) was mounted on the bed. This assembly was centered inside a 72 mm quadrature volume coil in transmit-only mode (Bruker Biospin, Inc, MA, USA). Mice were imaged using an accelerated spin-echo sequence (Turbo Rapid Acquisition with Relaxation Enhancement, TurboRARE) oriented in axial, sagittal, and coronal directions. The following parameters were used: TR/TE = 1250 ms/21.3 ms, RARE factor 8, MTX = 256 x 256, FOV 3 x 3 cm, 7-13 slices of 0.75-1 mm thick (as needed for full brain coverage), flip back enabled, and 3 signal averages. The acquisition time was approximately 2 min per scan.
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