Jsi124

Manufactured by Bio-Techne

The JSI124 is a compact and versatile laboratory instrument designed for a variety of analytical applications. It features a durable construction and intuitive user interface, making it a reliable tool for various research and testing needs.

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Lab products found in correlation

Mifepristone, by Merck Group (1 mentions) Pd169316, by Merck Group (1 mentions) Stat3 inhibitor, by Bio-Techne (1 mentions) Ly294002, by Bio-Techne (1 mentions) U0126, by Merck Group (1 mentions) Uo126, by Bio-Techne (1 mentions) Pyrvinium, by Merck Group (1 mentions) Pi 103, by Bio-Techne (1 mentions) Ly294002, by Cayman Chemical (1 mentions)

2 protocols using jsi124

Zebrafish Chemical Modulation of Tgfb1a

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For chemical treatments, 20 5-dpf zebrafish larvae fed with 10% cholesterol-infused diet were co-exposed with either 1 μM of JSI124, a Stat3 inhibitor (Tocris), or LY294002, a PI3K inhibitor (Tocris), for 7 days. For induction of tgfb1a expression from tgfb1a+ transgenic zebrafish, 1, 2 or 3 μM of mifeprisone (Sigma) were used to incubate 3-month-old fish for 6 weeks. For inhibition of Smad and Erk activity, 1 μM PD169316 (Sigma) or U0126 (Sigma) was added to tgfb1a+ zebrafish after 3 weeks of 3 μM mifepristone induction. All chemical dosages were selected based on the highest all-survival concentrations.

Yan C., Yang Q., Shen H.M., Spitsbergen J.M, & Gong Z. (2017). Chronically high level of tgfb1a induction causes both hepatocellular carcinoma and cholangiocarcinoma via a dominant Erk pathway in zebrafish. Oncotarget, 8(44), 77096-77109.

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Inhibitor-Mediated Modulation of Signaling Pathways

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Control fish were treated with DMSO (1:200). Mapk/Erk inhibitor, UO126 (Tocris Bioscience); PI3K/Akt inhibitor, Ly294002 (Cayman Chemical) and PI-103 (Tocris); EGF receptor inhibitor, PD153035; β-catenin inhibitor, pyrvinium (Sigma); and Jak/Stat inhibitor JSI-124 (Tocris) were used in this study. Fish were immersed in fish water containing the inhibitor (10 µM) or injected intravitreally through the front of the eye (1 µl of 1 µM).

Wan J., Zhao X.F., Vojtek A, & Goldman D. (2014). Retinal injury, growth factors and cytokines converge on β-catenin and pStat3 signaling to stimulate retina regeneration. Cell reports, 9(1), 285-297.

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