Excised mouse tumor tissues were fixed in paraffin and prepared for histological examination. After dewaxing, 4 μm paraffin-embedded sections were incubated with 3% H2O2 for 10 min at room temperature and then with 5% bovine serum albumin for 30 min at 37 °C. The sections were then stained with anti-mouse proliferating cell nuclear antigen expression (PCNA) monoclonal antibody (1:200 dilution, Boster, Wuhan, China), rat anti-mouse CD31 antibody (1:50 dilution, Abcam), and rabbit anti-mouse CXCL10/IP-10 antibody (1:100 dilution, Biosynthesis) and kept at 4 °C overnight. After three washes with PBS, the sections were stained with HRP-labeled goat anti-mouse, goat anti-rat, or goat anti-rabbit IgG secondary antibodies (Zhongshan Golden Bridge, China) and incubated for 30 min at 37 °C. Sections treated as described without the primary antibody were used as negative controls. The sections were counterstained with DAB, examined by microscopy (400×), and analyzed using Image-Pro Plus® software (Media Cybernetics).
Rat anti mouse cd31 antibody
Manufactured by Abcam
Sourced in China, United Kingdom, United States
Rat anti-mouse CD31 antibody is a primary antibody that specifically binds to the CD31 protein, also known as platelet endothelial cell adhesion molecule (PECAM-1), which is expressed on the surface of endothelial cells and platelets. This antibody can be used in various immunoassays to detect and quantify CD31 in mouse samples.
Automatically generated - may contain errors
Lab products found in correlation
Image pro plus software, by Media Cybernetics (1 mentions)
Cy5 conjugated goat anti rabbit secondary antibody, by Abcam (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Pd 10 disposable column, by GE Healthcare (1 mentions)
Alexa fluor 568 goat anti rat igg, by Thermo Fisher Scientific (1 mentions)
Cy5.5 n hydroxysuccinimide cy5.5 nhs ester, by Lumiprobe (1 mentions)
3 protocols using rat anti mouse cd31 antibody
1
Immunohistochemical Analysis of Mouse Tumor
2
Visualizing Tumor Vascular Permeability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In order to confirm that GVs were able to pass through the endothelial gaps in tumors, we used a fluorescent inverted microscope to observe DiI-dyed GVs in the tumor section. A mixture of DiI-labeled GVs and FITC-labeled lipid MBs were intravenously injected into the tumor-bearing mice, and the contrast imaging signals were observed in tumors using an ultrasound imaging system. When the contrast signals reached their peak, the mice were sacrificed and the tumors were immediately removed for sectioning into 10 μm slices. Slices were incubated with rat anti-mouse CD31 antibody (Abcam, Cambridge, UK) overnight at 4 °C, then incubated with Cy5-conjugated goat anti-rabbit secondary antibody (Abcam, Cambridge, UK) to visualize the vessels. The cell nuclei were stained with DAPI (Sigma Aldrich, St. Louis, MO, USA). Images were recorded using a fluorescent Inverted microscope (IX73, Olympus, Tokyo, Japan).
3
Radiolabeling of Dll4-Targeting Antibody
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!