N butanol

Bitespiramycin Tablets (Shanghai Tonglian Pharmaceutical Co., Ltd., Shanghai, China, Lot No. 0220200204); SPMs I, II, III and ISV-SPMs I, II, III standards (Shanghai Tonglian Pharmaceutical Co., Ltd., purity 98%); Acetonitrile (chromatographic grade; Fisher Chemical, Waltham, MA, USA); n-butanol (HPLC; Macklin, Shanghai, China); Methanol (chromatographic grade; Aladdin, Shanghai, China); Ammonium acetate (LC-MS; Aladdin, Shanghai, China); Ammonia (LC-MS; Aladdin, Shanghai, China); Azithromycin in methanol (internal standard, 100 μg/mL, purity 98%, Ehrenstorfer, London, UK); Sodium carboxymethyl cellulose (Jiangsu Anxin Food Ingredients Mall, Nanjing, China); Sodium heparin (Leagene, Beijing, China); Endothelial Cell Medium (ECM, Scienncell, Carlsbad, CA, USA); Pancreatin (Gibco, Waltham, MA, USA); DPBS 1X (Gibco, Waltham, MA, USA); Matrigel matrix (Corning, New York, NY, USA).

SGT and PRT samples were provided by Yunnan Shuangjiang Mengku Tea Co., Ltd. (Lincang, China). Detailed information on the tea samples is supplied in Table 1. To ensure the accuracy of the experimental results, all the tea samples used came from the same batch. A microfiltration membrane (0.45 μm) was purchased from Shanghai Xinya Purification material factory (Shanghai, China). Ultrafiltration centrifuge tubes were purchased from Millipore (Billerica, MA, USA). Caffeine, gallic acid (GA), (−)−gallocatechin, (−)−epigallocatechin, (+)−catechin, (−)−epigallocatechin gallate, (−)−epicatechin, (−)−gallocatechin gallate, (−)−epicatechin gallate, (−)−catechin gallate, glutamic acid, Folin−Ciocalteu’s phenol, and acetonitrile were purchased from Sigma−Aldrich (Shanghai, China). N−Butanol and ethyl acetate were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Theabrownin (TB, 93%) was purchased from Yunnan Tangren Biotechnology Co., Ltd. (Honghe, China). Bradford Protein Assay Kit was purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China).