Ab230509

BCPAP cells or C643 cells were harvested and lysed with RIPA buffer (Millipore). Immunoblotting was performed in a standard fashion. The following antibodies were used: β-actin (1 : 1000; cat #AF5003; Beyotime, China), NDUFB3 (1 : 500; cat #SC-393351; Santa Cruz, CA), MT-ND1 (1 : 1000, cat #ab181848; Abcam), MT-ND2 (1 : 1000, cat #PA5-37185; Invitrogen), MT-ND3 (1 : 1000, cat #ab192306; Abcam), MT-ND4 (1 : 1000, cat #ab219822; Abcam), MT-ND4L (1 : 1000, cat #PA5-103953; Invitrogen), MT-ND5 (1 : 1000, cat #ab230509; Abcam), MT-ND6 (1 : 1000, cat #PA5-109993; Invitrogen), GPX1 (1 : 1000, cat #ab22604; Abcam), MnSOD (1 : 1000, cat #ab68155; Abcam), CuMnSOD (1 : 1000, cat #ab13498; Abcam), PRDX1 (1 : 1000, cat #NBP1-82558; Novus), PRDX3 (1 : 1000, cat #NBP2-67043; Novus), PRDX6 (1 : 1000, cat #H00009588-M01; Novus), and α-tubulin (1 : 1000; cat# AF0001; Beyotime, China).

Western blotting analysis was performed as detailed previously.^{32 (link)} Antibodies used in this study include anti-YARS2 (ab228957, at a dilution of 1:1000), anti-MT-ND1 (ab181848, at a dilution of 1:2000), anti-MT-ND5 (ab230509, at a dilution of 1:1000), anti-MT-CO2 (ab79393, at a dilution of 1:5000), and anti-TOM20 (ab186735, at a dilution of 1:2000) from Abcam, anti-MT-ND6 (PA5-43532, at a dilution of 1:1000) and anti-MT-ND4 (PA5-97298, at a dilution of 1:1000) from ThermoFisher, anti- MT-CYTB (55090-1-AP, at a dilution of 1:1000) from Proteintech, anti-β-actin (AF5003, at a dilution of 1:2000) from Beyotime Institute of Biotechnology, and HRP-conjugated anti-rabbit secondary antibody (A0208, at a dilution of 1:1000) from Beyotime Institute of Biotechnology. Immunoreactive proteins were visualized using a chemiluminescent immunodetection system (ChemiDoc XRS). Image J was employed to analyze the grayscale values obtained by western blotting.