S 2366 l pyro glu l pro l arg p nitroanilide

Manufactured by Diapharma

S-2366 is a synthetic substrate for the measurement of enzyme activity. It consists of the amino acid sequence L-pyro-Glu-L-Pro-L-Arg linked to the chromogenic compound p-nitroanilide. The cleavage of the p-nitroanilide moiety by the target enzyme can be detected spectrophotometrically, allowing for the quantification of enzyme activity.

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Lab products found in correlation

Corn trypsin inhibitor, by Enzyme Research (3 mentions) S2302 h d prolyl l phenylalanyl l arginine p nitroanilide, by Diapharma (3 mentions) Streptavidin hrp, by Thermo Fisher Scientific (2 mentions) Hrp conjugated antihemagglutinin ha igg, by Thermo Fisher Scientific (2 mentions) Kaolin, by Thermo Fisher Scientific (2 mentions) Soybean trypsin inhibitor sbti, by Merck Group (2 mentions) Activated partial thromboplastin time reagent, by Diagnostica Stago (2 mentions) Horseradish peroxidase conjugated hrp goat antihuman fxii, by Enzyme Research (2 mentions) βfxiia, by Enzyme Research (1 mentions) Dextran sulfate, by Merck Group (1 mentions) Soybean trypsin inhibitor, by Merck Group (1 mentions) Polyethyleneimine, by Merck Group (1 mentions) ε aminocaproic acid εaca, by Merck Group (1 mentions) α thrombin, by Enzyme Research (1 mentions) Ellagic acid, by Thermo Fisher Scientific (1 mentions) Argatroban, by GlaxoSmithKline (1 mentions) Ptt a reagent, by Diagnostica Stago (1 mentions) Plasmin, by Enzyme Research (1 mentions) Fxiia, by Enzyme Research (1 mentions) Fibrillar collagen type 1, by Chrono-Log (1 mentions)

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S-2366 (12 citations)

4 protocols using s 2366 l pyro glu l pro l arg p nitroanilide

Quantification of FXII and PK Activation

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Materials used were as follows: normal human plasma (Precision BioLogic, Dartmouth, Canada); FXII-deficient plasma (George King Biomed); FXII, FXIIa, βFXIIa, PK, PKa, α-thrombin, corn trypsin inhibitor, and horseradish peroxidase–conjugated goat anti-human FXII and anti-human PK (Enzyme Research Laboratory, South Bend, IN); dextran sulfate (500 000 Da), polyethyleneimine (PEI; 750 000 Da), soybean trypsin inhibitor, and ε-aminocaproic acid (ε-ACA; Sigma-Aldrich, St. Louis, MO); ellagic acid (ACROS Organics, The Hague, Netherlands); S2302 (H-d-prolyl-l-phenylalanyl-l-arginine-p-nitroanilide) and S-2366 (l-pyro-Glu-l-Pro-l-Arg-p-nitroanilide; DiaPharma, Westchester, OH); PTT-A reagent (Diagnostica Stago, Asnieres-sur-Seine, France); argatroban (GlaxoSmithKline, Brentford, United Kingdom); polyphosphate (poly-P; 200-1300 units; provided by James Morrissey, University of Michigan); and horseradish peroxidase–conjugated antihemagglutinin immunoglobulin G (IgG; Invitrogen). Monoclonal IgGs 15H8 and 1B2^{8,18 (link)} are described.

Shamanaev A., Ivanov I., Sun M.F., Litvak M., Srivastava P., Mohammed B.M., Shaban R., Maddur A., Verhamme I.M., McCarty O.J., Law R.H, & Gailani D. (2022). Model for surface-dependent factor XII activation: the roles of factor XII heavy chain domains. Blood Advances, 6(10), 3142-3154.

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Coagulation Factors and DNA Isolation

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Human FXII, FXIIa, PK, α-kallikrein and plasmin were from Enzyme Research Laboratory (South Bend, IN). Human factor XIa was from Haematologic Technologies (Burlington, VT). Type I fibrillar collagen was from Chrono-Log (Havertown, PA). Anhydrous iron (III) chloride (FeCl₃, molecular mass 160.20 Daltons) and delipidated bovine serum albumin (BSA) was from Sigma-Aldrich. Phosphatidylcholine:phosphatidylserine (PC/PS) vesicles were form Avanti Polar Lipids (Alabaster, Alabama). S-2366 (L-pyro-Glu-L-Pro-L-Arg-p-nitroanilide) and S2302 (H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroaniline dihydrochloride.) were from DiaPharma (West Chester, OH). Z-Gly-Gly-Arg-AMC was from Bachem (Torrance, CA). PTT A silica-based activated partial thromboplastin time (aPTT) reagent was from Diagnostic Stago (Parsippany, NJ). Human genomic DNA was isolated from blood leukocytes by conventional phenol:chloroform extraction.

Kokoye Y., Ivanov I., Cheng Q., Matafonov A., Dickeson S.K., Mason S., Sexton D.J., Renné T., McCrae K., Feener E.P, & Gailani D. (2016). A Comparison of the Effects of Factor XII Deficiency and Prekallikrein Deficiency on Thrombus Formation. Thrombosis research, 140, 118-124.

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Assay of Contact Activation Factors

Cited 1 time

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Human pooled normal plasma (Precision BioLogic). FXII-deficient plasma (George King Biomed). FXII, FXIIa, βFXIIa, PK, PKa, XI, XIa, corn-trypsin inhibitor (CTI), and horseradish peroxidase-conjugated (HRP) goat antihuman FXII (Enzyme Research Laboratory). Soybean trypsin inhibitor (SBTI) (Sigma-Aldrich). S2302 (H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide) and S-2366 (L-pyro-Glu-L-Pro-L-Arg-p-nitroanilide (DiaPharma). Activated partial thromboplastin time (aPTT) reagent (Diagnostica Stago). Kaolin (ACROS Organics). Phe-Pro-Arg-chloromethylketone (FPRCK) (Prolytix). Long-chain PolyP with a broad range of polymer lengths (200-1300 orthophosphate units), and size-fractionated short-chain PolyP (PolyP-31, 27-35 units) have been described [12 (link)]. HRP-conjugated antihemagglutinin (HA) IgG (Invitrogen). Monoclonal biotinylated anti-FXI IgG 14E11 has been described [13 (link)]. Streptavidin-HRP (Thermo Scientific).

Hara-Kudo Y., Ikedo M., Kodaka H., Nakagawa H., Goto K., Masuda T., Konuma H., Kojima T, & Kumagai S. (2000). Selective Enrichment with a Resuscitation Step for Isolation of Freeze-Injured Escherichia coli O157:H7 from Foods. Applied and Environmental Microbiology, 66(7), 2866-2872.

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Intrinsic Coagulation Pathway Analysis

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Human pooled normal plasma (Precision BioLogic). FXII-deficient plasma (George King Biomed). FXII, FXIIa, βFXIIa, PK, PKa, XI, XIa, corn-trypsin inhibitor (CTI), and horseradish peroxidase-conjugated (HRP) goat antihuman FXII (Enzyme Research Laboratory). Soybean trypsin inhibitor (SBTI) (Sigma-Aldrich). S2302 (H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide) and S-2366 (L-pyro-Glu-L-Pro-L-Arg-p-nitroanilide (DiaPharma). Activated partial thromboplastin time (aPTT) reagent (Diagnostica Stago). Kaolin (ACROS Organics). Phe-Pro-Arg-chloromethylketone (FPRCK) (Prolytix). Long-chain PolyP with a broad range of polymer lengths (200-1300 orthophosphate units), and size-fractionated short-chain PolyP (PolyP-31, 27-35 units) have been described [12] . HRP-conjugated antihemagglutinin (HA) IgG (Invitrogen). Monoclonal biotinylated anti-FXI IgG 14E11 has been described [13] . Streptavidin-HRP (Thermo Scientific).

Shamanaev A., Litvak M., Cheng Q., Ponczek M., Dickeson S.K., Smith S.A., Morrissey J.H, & Gailani D. (2023). A site on factor XII required for productive interactions with polyphosphate. Journal of thrombosis and haemostasis : JTH, 21(6).

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