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Flp mice

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FLP mice are a genetically modified mouse model used for conditional gene manipulation. The FLP recombinase enzyme, derived from the yeast Saccharomyces cerevisiae, is expressed in these mice, enabling site-specific DNA recombination at FRT sites.

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Lab products found in correlation

αmhc mercremer, by Jackson ImmunoResearch (1 mentions) Villin cre mice, by Jackson ImmunoResearch (1 mentions) Rosacreert2, by Jackson ImmunoResearch (1 mentions) Pv cre, by Jackson ImmunoResearch (1 mentions) Ribotag, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions)

5 protocols using flp mice

1

Systematic Generation of Btbd9 KO Mice

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The homozygous Btbd9 KO male mice used in MRI imaging were generated as described previously (DeAndrade et al., 2012a (link)). The systematic Btbd9 KO mice used in the electrophysiological recording were generated from a line of Btbd9 loxP mice imported from the European Mouse Mutant Archive (EMMA; ID: 05554). In this line, the fourth exon of the Btbd9 gene was flanked by loxP sites (floxed). We first removed neomycin selection cassette by crossing with FLP mice (The Jackson Laboratory stock 126 no. 003946) to obtain Btbd9 loxP mice, which was then crossed with a general cre deletor to obtain Btbd9 KO allele. Heterozygous Btbd9 KO mice were interbred to produce experimental homozygous Btbd9 KO mice and the wild-type (WT) littermate controls.
Lyu S., Xing H., DeAndrade M.P., Liu Y., Perez P.D., Yokoi F., Febo M., Walters A.S, & Li Y. (2019). The Role of BTBD9 in Striatum and Restless Legs Syndrome. eNeuro, 6(5), ENEURO.0277-19.2019.
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2

Generation of SENP2 Conditional Knockout Mice

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SENP2fxN/fxN mice were generated as shown in Figure S1. In brief, a genomic clone containing exons 11–16 of the mouse SENP2 gene was isolated from a BAC library and cloned into a vector using homologous recombination (Zhang et al., 2002b (link)). A cassette containing a loxP-flanked NeoR gene expressed from the phosphoglycerate kinase promoter (PGK-NeoR) was cloned into intron 14 to obtain the final targeting vector. Following germline transmission, SENP2fxN/+ mice were mated to obtain SENP2fxN/fxN knocking in mice and wild-type littermates. After SENP2fxN/fxN crossing with FLP mice (Jackson lab), the final allele without Neo cassette (SENP2fx/fx) was obtained. We established mouse models with neuron-specific (SENP2fx/fx-Thy1) and cardiomyocyte-specific (SENP2fx/fx-MHC) deletion of SENP2. SENP2fx/fx mice were crossed with mice carrying Thy1 promoter (Thy1-cre/ERT2, -EYFP, Jackson lab) or MHC promoter (α-MHC-MerCreMer, Jackson lab) and a fusion protein containing Cre and mutated estrogen receptor.
Qi Y., Wang J., Bomben V.C., Li D.P., Chen S.R., Sun H., Xi Y., Reed J.G., Cheng J., Pan H.L., Noebels J.L, & Yeh E.T. (2014). Hyper-SUMOylation of the Kv7 Potassium Channel Diminishes the M-Current Leading to Seizures and Sudden Death. Neuron, 83(5), 1159-1171.
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3

Generation of Conditional Knockout Mice

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Bap1tm2a(EUCOMM)hmgu, Pbrm1tm1a(EUCOMM)Wtsi ES cells were purchased from European Conditional Mouse Mutagenesis Program and injected into the cavity of Day 3.5 blastocysts from C57Bl/6N mice at the University of Texas Southwestern Medical Center Transgenic Core. Male chimeras were mated with C57BL/6NTac female mice to generate germline transmission. Mice heterozygous for the Bap1 or Pbrm1 null allele (Bap1/Pbrm1 lacZ/+) were used for β-galactosidase staining to determine Bap1/Pbrm1 expression. Pbrm1-LacZ/+ mice were mated with FLP mice (Jackson Laboratory) to introduce FRT recombinase reactivity for excision of the LacZ element to generate floxed Pbrm1 mice. Floxed VhlF/F mice were kindly provided by Dr. Volker H. Haase (Vanderbilt University Medical Center, Nashville, TN) (19 (link)). Bap1F/F mice were kindly provided by Dr. Vishva M. Dixit (Genentech, San Francisco, CA) (61 (link)). Sglt2-Cre mice were kindly provided by Dr. Michel Tauc (University of Nice-Sophia Antipolis, France) (47 (link)). Villin-Cre mice (Jackson Laboratory; JAX 004586) were kindly provided by Dr. Joshua Mendell (University of Texas Southwestern Medical Center). Rosa26-CAG-loxP-stop-loxP-tdTomato mice were from the Jackson Laboratory. Mouse protocols were approved by the Institutional Animal Care and Use Committee (APN#2015-100932) at the University of Texas Southwestern Medical Center.
Gu Y.F., Cohn S., Christie A., McKenzie T., Wolff N., Do Q.N., Madhuranthakam A.J., Pedrosa I., Wang T., Dey A., Busslinger M., Xie X.J., Hammer R.E., McKay R.M., Kapur P, & Brugarolas J. (2017). Modeling Renal Cell Carcinoma in mice: Bap1 and Pbrm1 Inactivation Drive Tumor Grade. Cancer discovery, 7(8), 900-917.
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4

Generation of Zfp809 Knockout Mice

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Zfp809GT mice were generated by injection of 129S2/SvPas ESCs (German Gene Trap Consortium, no. F065A07), which contain a Zfp809 allele with a gene trap vector (pT1ATG βGeo) inserted between exons 1 and 2, into B6D2F2 blastocysts. Chimeric mice were intercrossed to obtain Zfp809GT/+ and Zfp809GT/GT mice. Zfp809KO-first mice were generated by injection of C57/Bl6 ESCs harboring a conditional Zfp809 knockout allele (Knockout Mouse Project [KOMP] Repository, University of California at Davis) into C57/Bl6 blastocysts. Zfp809KO-first mice were crossed with Flp mice (Jackson Laboratory, stock no. 003946) to facilitate germline FLP-FRT recombination and the birth of Zfp809FL mice. Zfp809FL mice were crossed with mice harboring either constitutively active Cre (Jackson Laboratory, stock no. 003724) or a 4-OHT-inducible RosaCreERT2 gene (Jackson Laboratory, stock no. 004847) to generate Zfp809 knockout mice or inducible Zfp809FL/FL; RosaCreERT2 mice, respectively.
Wolf G., Yang P., Füchtbauer A.C., Füchtbauer E.M., Silva A.M., Park C., Wu W., Nielsen A.L., Pedersen F.S, & Macfarlan T.S. (2015). The KRAB zinc finger protein ZFP809 is required to initiate epigenetic silencing of endogenous retroviruses. Genes & Development, 29(5), 538-554.
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5

Generation of Nrxn123-PtprDFS Sextuple Knockout Mice

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LAR-PTPR triple cKO mice and Nrxn123 triple cKO mice were generated as previously described30 (link),33 (link). Briefly, Nrxn123 triple cKO mice were generated by flanking exon 18 with FloxP site30 (link). PtprDFS 3cKO mice were obtained by crossing Ptprd cKO mice (Ptprdtm2a(KOMP)Wtsi, colony prefix MEXY, ESC clone ID: EPD0581_9_D04, RRID: IMSR_EM:11805, then crossed to Flp mice (Jackson Laboratory, JAX:005703, RRID: IMSR_JAX:005703), Ptprs cKO mice (Ptprs_tm1c_D11, ES cell clone ID: DEPD00535_1_D11, RRID: IMSR_KOMP:CSD76529-1c-Mbp), and PTPRF cKO mice (generated by flanking exon 4 with loxP sites)33 (link).
Nrxn123-PtprDFS sextuple 6cKO mice were generated by crossing Nrxn123 3cKO and PtprDFS 3cKO mice over multiple generations. PV-Cre (Jackson Lab JAX # 017320) mice were included in these crosses to generate the Nrxn123-PtprDFS 6cKO/PV-Cre mice. C57BL/6 J (Jackson Laboratory, JAX #000664), PV-Cre L7-cre (Jackson Laboratory, JAX #004146), and Ribotag (Jackson Laboratory, JAX #029977) mice were purchased from the Jackson laboratory. Mice were group-housed on a 12 h light-dark cycle with access to food and water ad libitum. Male and female mice were used for all experiments.
Sclip A, & Südhof T.C. (2023). Combinatorial expression of neurexins and LAR-type phosphotyrosine phosphatase receptors instructs assembly of a cerebellar circuit. Nature Communications, 14, 4976.
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