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P-LATS is a phospho-specific antibody that recognizes phosphorylated LATS1 and LATS2 proteins. LATS1 and LATS2 are core components of the Hippo signaling pathway, which plays a crucial role in regulating cell proliferation, apoptosis, and organ size control.

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Lab products found in correlation

Lats2, by Cell Signaling Technology (2 mentions) Lats1, by Cell Signaling Technology (2 mentions) P 4ebp1, by Cell Signaling Technology (2 mentions) H3k27ac, by Cell Signaling Technology (1 mentions) Ascl1, by Abcam (1 mentions) Insm1, by Abcam (1 mentions) Rcor1, by Santa Cruz Biotechnology (1 mentions) Yap taz, by Santa Cruz Biotechnology (1 mentions) H3k4me3, by Cell Signaling Technology (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Dasatinib, by Selleck Chemicals (1 mentions) Etoposide, by Selleck Chemicals (1 mentions) P chk1, by Cell Signaling Technology (1 mentions) P cdc25c, by Cell Signaling Technology (1 mentions) P chk2, by Cell Signaling Technology (1 mentions) Pd0325091, by Selleck Chemicals (1 mentions) P h2ax, by Cell Signaling Technology (1 mentions) Anti yap taz, by Cell Signaling Technology (1 mentions) Survivin, by Cell Signaling Technology (1 mentions)

4 protocols using p lat

1

Comprehensive Immunohistochemistry Profiling

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The antibodies used were: ASCL1 (Abcam, #ab211327), INSM1 (Abcam, #ab170876), E2F7 (Abcam, ab245655), RB1 (Abcam, ab181616), SYP (Santa Cruz, #sc-17750), RCOR1 (Santa Cruz, #sc-376567), CCN1 (Santa Cruz, #sc-374129), CCN2 (Santa Cruz, #sc-365970), YAP/TAZ (Santa Cruz, #sc-101199), P53 (Santa Cruz, #sc-126), E2F1 (Santa Cruz, #sc-251), GAPDH (Santa Cruz, #sc-47724), LATS1 (Cell signaling, #3477), LATS2 (Cell signaling, #5888), p-LATS (Cell signaling, #8654), H3 (Cell signaling, #4499), H3K27ac (Cell signaling, #8173), H3K4me3 (Cell signaling, #9751), VIN (Cell signaling, #13901), Flag (Sigma, #1804),
Wu Z., Su J., Li F.L., Chen T., Mayner J., Engler A., Ma S., Li Q, & Guan K.L. (2023). YAP silencing by RB1 mutation is essential for small-cell lung cancer metastasis. Nature Communications, 14, 5916.
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2

Investigating Signaling Pathways in Cancer

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Dasatinib, BIBW2992, PD0325091 and Etoposide were purchased from Selleck Chemicals (Houston, TX) and prepared as 20 mM stock solutions in DMSO. Antibodies used included anti-YAP-TAZ, pLats, Lats1, Lats2, pMST1/2, pH2AX, MEK, pMEK(S217/221), ERK, pERK(T202/Y204), pRb, pS6, LC3A/B, ATM, pChk1, Chk1, pChk2, Chk2, pCDC25c, pATRIP, p27, p21, pp38, β-catenin, cyclin D1, survivin, p4EBP1, pSMAD2(S465–467), p70S6K, pCDC2, CDC6, γH2AX, ATR, pDNA-PK(S2056) and DNA-PK, (Cell Signaling Technology, Danvers, MA); BRAF and cyclin E (Santa Cruz, Dallas, TX) and ATM, Flag M2 and β-actin (Sigma-Aldrich, St. Louis, MO); pATM(S1981) (GeneTex, Irvine, CA). Predesigned siRNAs of the target genes were purchased from Dharmacon (Pittsburgh, PA) or Thermo Scientific (Rodckford, IL). pcDNA4-Chk1-Flag and pCMV5-TOPO-3Xflag-TAZ plasmids were purchased from Addgene (Cambridge, MA). WTBRAF plasmid was provided by Dr. W. Kolch (Systems Biology Irland and The Conway Institute, University College Dublin).
Peng S., Sen B., Mazumdar T., Byers L.A., Diao L., Wang J., Tong P., Giri U., Heymach J.V., Kadara H.N, & Johnson F.M. (2015). Dasatinib induces DNA damage and activates DNA repair pathways leading to senescence in non-small cell lung cancer cell lines with kinase-inactivating BRAF mutations. Oncotarget, 7(1), 565-579.
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3

Characterization of Signaling Pathways in Human Myeloma

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Human myeloma IgE (huIgE, Calbiochem, La Jolla, CA) was biotinylated in the NIAID Core Facility. The protein-specific antibodies used in confocal microscopy were: mTOR (10343, IBL, Minneapolis, MN) and rictor (NB100-612, Novus Biologicals, Littleton, CO). The β-actin-specific antibody for immunoblotting was from Cell Signaling Technology (Beverley, MA) and Lyn-specific antibody for immunoblotting from Santa Cruz Biotechnology (Santa Cruz, CA). The conjugated protein-specific antibodies were: fluorophore-conjugated antibodies (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA), horseradish peroxidase-conjugated rabbit IgG (Amersham Biosciences, Piscataway, NJ), and mouse IgG Fc-specific antibody (Sigma-Aldrich). The phosphoprotein-specific antibodies for immunoblotting were: p-mTOR(Ser2448), p-mTOR(Ser2481), p-rictor (Thr1135), p-Akt(Thr308), p-Akt(Ser473), p-LAT(Tyr171), p-S6 ribosomal protein (S6RP) (Ser240/244), p-4E-BP1(Thr37/46), and rictor (Cell Signaling Technology), and p-PLCγ1(Tyr783) (Invitrogen). The blocking donkey serum was from Santa Cruz Biotechnology and Torin1 (37 (link)) was a gift of Dr. Nathanael S. Gray, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA. FITC-conjugated phalloidin and other chemicals and reagents were from Sigma-Aldrich.
Smrz D., Cruse G., Beaven M.A., Kirshenbaum A., Metcalfe D.D, & Gilfillan A.M. (2014). Rictor negatively regulates FcεRI-induced mast cell degranulation. Journal of immunology (Baltimore, Md. : 1950), 193(12), 5924-5932.
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4

Immunoprecipitation and Immunoblotting of T-Cell Signaling Proteins

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Antibodies against the indicated proteins were purchased from Cell Signaling Technology: Lck (Cat. 2752), pSrc (Cat. 2101), pZAP70 (Y319, Cat. 2717), pZAP-70 (Y493, Cat. 2704), ZAP-70 (Cat. 3165), pErk1/2 (Cat. 9101), Erk1/2 (Cat. 4695), GAPDH (Cat. 5174), Calnexin (Cat. 2679), LAT (Cat. 9166), pLAT (Cat. 3581), PLC-γ1 (Cat. 2822), pPLCγ1 (Cat. 2821), SLP-76 (Cat. 70896) and GRB2 (Cat. 3972). Antibodies against pSLP-76 were purchased from Invitrogen, Cat. PA5-39759. The following antibodies were used for immunoprecipitation: anti-Lck clone 3A5 (EMD Millipore, Cat. 05-435) and anti-ZAP-70 clone 35A.2 (EMD Millipore, Cat. 05-253). The following reagents were used: Anti-human CD3 antibody (OKT3) (eBioscience, Cat. 14-0037-82), Pierce Protein A agarose beads (Thermo Scientific, Cat. 20333), Hydroxylamine hydrochloride (Sigma, Cat.159417), Methyl methanethiosulfonate (MMTS) (Sigma, Cat. 208795), n-Dodecyl β-D-maltoside (DDM) (Sigma, Cat. D4641), EZ-Link HPDP-biotin (Thermo Scientific, Cat. 21341), Streptavidin agarose beads (Invitrogen, S951), Poly-L-lysine (Sigma, Cat. P4707), Phosphatase Inhibitor Cocktail 2 (Sigma, Cat. P5726), Complete Protease Inhibitor Cocktail tablets (Roche, Cat. 11836170001) and ML211 (Cayman Chemical, Cat. 17630).
Tewari R., Shayahati B., Fan Y, & Akimzhanov A.M. (2021). T cell receptor–dependent S-acylation of ZAP-70 controls activation of T cells. The Journal of Biological Chemistry, 296, 100311.
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