Dynasore (Sigma-Aldrich), RNase A (Roche), Proteinase K (Fisher Scientific), CM-DiI (Thermo Fisher Scientific, C7001), and human HepaCAM protein extracellular domain (ECD, amino acid sequence 1-240, 16047-H08H) (Sino Biological Inc.) were used in this study. Dynasore (stock 50 mM) was prepared in DMSO and diluted 1000x in neuronal growth medium for treatment. Antibodies (final concentration 100 μg/ml) were mixed with neuronal growth medium and added onto primary neuronal cultures 2 h before A-Exo. treatment with exosomes. HepaCAM ECD coating is described below. Neuronal treatment with various drugs and/or exosomes was generally at DIV 3–4 for 24 h unless specifically described in main text.
Human hepacam protein extracellular domain ecd
Manufactured by Sino Biological
The Human HepaCAM protein extracellular domain (ECD) is a recombinant protein product. HepaCAM is a cell adhesion molecule that plays a role in cell-cell interactions. The extracellular domain of the protein is produced using a recombinant expression system.
Automatically generated - may contain errors
2 protocols using human hepacam protein extracellular domain ecd
1
Neuronal Exosome Internalization Assay
2
Neuronal Exosomes Internalization Assay
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Dynasore (Sigma Aldrich), RNase A (Roche), Proteinase K (Fisher Scientific), CM-DiI (Thermo Fisher Scientific, C7001), and human HepaCAM protein extracellular domain (ECD, amino acid sequence 1–240, 16047-H08H) (Sino Biological Inc.) were used in this study. Dynasore (stock 50 mM) was prepared in DMSO and diluted 1000x in neuronal growth medium for treatment. Antibodies (final concentration 100 μg/mL) were mixed with neuronal growth medium and added onto primary neuronal cultures 2 hours before A-Exo. treatment with exosomes. HepaCAM ECD coating is described below. Neuronal treatment with various drugs and/or exosomes was generally at DIV 3–4 for 24h unless specifically described in main text.
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