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Digital sonifier model 250

Manufactured by Emerson
Sourced in United States

The Digital Sonifier model 250 is a laboratory equipment designed for cell disruption and sample preparation. It utilizes ultrasonic waves to break down and homogenize a variety of samples, including cells, tissues, and other materials. The device features a digital control panel for adjusting parameters such as sonication time and power.

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19 protocols using digital sonifier model 250

1

Conductive Carbon Nanotube-Reinforced Resin

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EXAMPLE 9

As another example, 23 mg of single-walled carbon nanotubes (SWCNT) obtained from OCSiAl (Palo Alto, Calif.) was added to 80 mL of Vorex resin (MadeSolid) and the suspension was power sonicated for 5 minutes with a Branson (Danbury, Conn.) Digital Sonifier model 250 fitted with a model 102C probe. This sonicated resin mixture was placed in the build vat of a commercial stereolithographic printer (Form1 from FormLabs, Boston, Mass.) and used to produce a 1 mm thick×10 mm wide by 50 mm long strip in which the nanotubes appeared to be very uniformly distributed, with no CNT agglomeration visible. The highly-flexible finished test strip was washed with IPA and then cured in sunlight for one hour. The conductivity of the test strip was then measured using a multimeter to obtain the electrical resistivity. This highly agglomerated test strip exhibited an electrical resistance of ranging from about 1 megaohm to infinity.

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2

Isolation and Enrichment of Nascent DNA

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Genomic DNA was isolated by incubating cells in SDS-PK buffer (0.5% (wt/vol) sodium dodecyl sulfate (SDS), 50 mM Tris-HCL, 0.01M EDTA, 1M NaCl and 0.2 mg/ml proteinase K) at 56 °C for 2 h. Phenol-chloroform extraction was performed and genomic DNA was precipitated in 1 volume of isopropanol at -20 °C. DNA was pelleted, washed with ethanol and resuspended in TE buffer. Genomic DNA was fragmented to an average size of 200 bp using a Branson Digital Sonifier (Model 250) and a Branson 101-148-063 microtip. DNA was sonicated using 40% amplitude for a total of 4 minutes. A double-antibody immunoprecipitation was performed to isolate nascent (BrdU labeled) strands of DNA. For each immunoprecipitation reaction, at least 120 ng of sonicated DNA was used. DNA was first heat denatured at 95 °C for 5 min and rapidly cooled on ice. 12.5 μg primary antibody (mouse anti-BrdU, BD Biosciences Pharmingen, cat. no. 555627) was added to the ssDNA suspension with constant rocking for 20 minutes followed by addition of 20 μg of secondary antibody (rabbit anti-mouse IgG, Sigma, cat. no. M-7023) for 20 min. Antibody-DNA complexes were then pelleted, resuspended and incubated in digestion buffer (50mM Tris-HCl, 0.01M EDTA, 0.5% SDS and 0.25 mg/ml proteinase K) at 37 °C overnight. Nascent ssDNA was subsequently purified by phenol-chloroform extraction and ethanol precipitation.
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3

Flexible Carbon Nanotube Composite Printing

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EXAMPLE 10

As another example, 60 mg of single-walled carbon nanotubes (SWCNT) obtained from OCSiAl (Palo Alto, Calif.) was added to 60 mL of Vorex resin (MadeSolid) to obtain a final SWCNT concentration of about 0.1% w/v and the suspension was power sonicated for 5 minutes with a Branson (Danbury, Conn.) Digital Sonifier model 250 fitted with a model 102C probe. This sonicated resin mixture was placed in the build vat of a commercial stereolithographic printer (Form1 from FormLabs, Boston, Mass.) and used to produce a 1 mm thick×10 mm wide by 50 mm long strip in which the nanotubes appeared to be very uniformly distributed, with no CNT agglomeration visible. The highly-flexible finished test strip was washed with IPA and then cured in sunlight for one hour. The conductivity of the test strip was then measured using a multimeter to obtain the electrical resistance. This highly agglomerated test strip exhibited an electrical resistance of about one megaohm over the entire sample length.

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4

Fabrication of Conductive Polymer Composites

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Example 11

As another example, 48 mL of the SWCNT-containing suspension from 60 mg of single-walled carbon nanotubes (SWCNT) obtained from OCSiAI (Palo Alto, Calif.) was added to 60 mL of Vorex resin (MadeSolid) to obtain a final SWCNT concentration of about 0.1% w/v and the suspension was power sonicated for 5 minutes with a Branson (Danbury, Conn.) Digital Sonifier model 250 fitted with a model 1020 probe. This sonicated resin mixture was placed in the build vat of a commercial stereolithographic printer (Form 1 from Form Labs, Boston, Mass.) and used to produce a 1 mm thick×10 mm wide by 50 mm long strip in which the nanotubes appeared to be very uniformly distributed, with no CNT agglomeration visible. The highly-flexible finished test strip was washed with isopropanol and then cured in sunlight for one hour. The conductivity of the test strip was then measured using a four-probe multimeter (R-Chek, EDTM Inc.) to obtain the electrical resistance. This test strip exhibited an electrical resistance of about one megaohm over the entire sample length.

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5

Fabrication of Conductive SWCNT-Embedded Resin Strips

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Example 12

As another example, 60 mg of single-walled carbon nanotubes (SWCNT) obtained from OCSiAI (Palo Alto, Calif.) was added directly to 50 mL of Vorex resin (MadeSolid) to obtain a final SWCNT concentration of about 0.12% Vorex and the suspension was power sonicated for 3 minutes with a Branson (Danbury, Conn.) Digital Sonifier model 250 fitted with a model 102C probe. This sonicated resin mixture was placed in the build vat of a commercial stereolithographic printer (Form 1 from FormLabs, Boston, Mass.) and used to produce a 1 mm thick×10 mm wide by 100 mm long strip in which the nanotubes appeared to be very uniformly distributed, with no CNT agglomeration visible. The highly-flexible finished test strip was washed with isopropanol and then cured in sunlight for one hour. The conductivity of the test strip was then measured using a using a four-probe multimeter (R-Chek, EDTM Inc.) to obtain the electrical resistance. This test strip exhibited an initial electrical resistance of about 400-600 kΩ over the entire 10-cm sample length, which fell to 200-300 kΩ over the entire 10-cm sample length after sitting overnight.

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6

Fabrication of Conductive SWCNT-Resin Composites

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Example 10

As another example, 60 mg of single-walled carbon nanotubes (SWCNT) obtained from OCSiAI (Palo Alto, Calif.) was added to 60 mL of Vorex resin (MadeSolid) to obtain a final SWCNT concentration of about 0.1% w/v and the suspension was power sonicated for 5 minutes with a Branson (Danbury, Conn.) Digital Sonifier model 250 fitted with a model 102C probe. This sonicated resin mixture was placed in the build vat of a commercial stereolithographic printer (Form 1 from FormLabs, Boston, Mass.) and used to produce a 1 mm thick×10 mm wide by 50 mm long strip in which the nanotubes appeared to be very uniformly distributed, with no CNT agglomeration visible. The highly-flexible finished test strip was washed with IPA and then cured in sunlight for one hour. The conductivity of the test strip was then measured using a multimeter to obtain the electrical resistance. This highly agglomerated test strip exhibited an electrical resistance of about one megaohm over the entire sample length.

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7

ChIP Assay and DNA Transfection in HeLa Cells

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ChIP assays were performed as described previously [20 (link)]. DNA transfection of HeLa cells was performed as described above. At 120 h after the first transfection, cells were crosslinked with 1% formaldehyde for 10 min at room temperature. Fixation was completed following the addition of glycine with a final concentration of 200 mM. The crosslinked genomic DNA was sheared by sonication using a Digital Sonifier Model 250 (Branson). The DNA-protein complexes were immunoprecipitated with Dynabeads Protein G (Invitrogen). An antibody directed against Histone H3 (tri methyl K9) (Abcam) was used. The precipitated DNA was analyzed by real-time PCR using the primers 5'-GTTCGCCAAAGGAAAAGCAGG-3' and 5'-GTGTCTGTCTCTCCCGGATGTC-3', which target a region located upstream of the transcription start site of the SERPINE1 promoter, or primers 5'-GCCCCTGTTTACGGAGCATTTC-3' and 5'-TGCGTGATACTGGGCTAGGAAC-3' which target the genomic region (ch10:79154928–79155075).
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8

Fabrication of SWCNT-Reinforced Polymer Test Strips

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EXAMPLE 12

As another example, 60 mg of single-walled carbon nanotubes (SWCNT) obtained from OCSiAl (Palo Alto, Calif.) was added directly to 50 mL of Vorex resin (MadeSolid) to obtain a final SWCNT concentration of about 0.12% w/v and the suspension was power sonicated for 3 minutes with a Branson (Danbury, Conn.) Digital Sonifier model 250 fitted with a model 102C probe. This sonicated resin mixture was placed in the build vat of a commercial stereolithographic printer (Form1 from FormLabs, Boston, Mass.) and used to produce a 1 mm thick×10 mm wide by 100 mm long strip in which the nanotubes appeared to be very uniformly distributed, with no CNT agglomeration visible. The highly-flexible finished test strip was washed with isopropanol and then cured in sunlight for one hour. The conductivity of the test strip was then measured using a using a four-probe multimeter (R-Chek, EDTM Inc.) to obtain the electrical resistance. This test strip exhibited an initial electrical resistance of about 400-600 kΩ over the entire 10-cm sample length, which fell to 200-300 kΩ over the entire 10-cm sample length after sitting overnight.

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9

Isolation and Enrichment of Nascent DNA

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Genomic DNA was isolated by incubating cells in SDS-PK buffer (0.5% (wt/vol) sodium dodecyl sulfate (SDS), 50 mM Tris-HCL, 0.01M EDTA, 1M NaCl and 0.2 mg/ml proteinase K) at 56 °C for 2 h. Phenol-chloroform extraction was performed and genomic DNA was precipitated in 1 volume of isopropanol at -20 °C. DNA was pelleted, washed with ethanol and resuspended in TE buffer. Genomic DNA was fragmented to an average size of 200 bp using a Branson Digital Sonifier (Model 250) and a Branson 101-148-063 microtip. DNA was sonicated using 40% amplitude for a total of 4 minutes. A double-antibody immunoprecipitation was performed to isolate nascent (BrdU labeled) strands of DNA. For each immunoprecipitation reaction, at least 120 ng of sonicated DNA was used. DNA was first heat denatured at 95 °C for 5 min and rapidly cooled on ice. 12.5 μg primary antibody (mouse anti-BrdU, BD Biosciences Pharmingen, cat. no. 555627) was added to the ssDNA suspension with constant rocking for 20 minutes followed by addition of 20 μg of secondary antibody (rabbit anti-mouse IgG, Sigma, cat. no. M-7023) for 20 min. Antibody-DNA complexes were then pelleted, resuspended and incubated in digestion buffer (50mM Tris-HCl, 0.01M EDTA, 0.5% SDS and 0.25 mg/ml proteinase K) at 37 °C overnight. Nascent ssDNA was subsequently purified by phenol-chloroform extraction and ethanol precipitation.
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10

SWCNT-Reinforced Resin 3D Printing

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EXAMPLE 11

As another example, 48 mL of the SWCNT-containing suspension from 60 mg of single-walled carbon nanotubes (SWCNT) obtained from OCSiAl (Palo Alto, Calif.) was added to 60 mL of Vorex resin (MadeSolid) to obtain a final SWCNT concentration of about 0.1% w/v and the suspension was power sonicated for 5 minutes with a Branson (Danbury, Conn.) Digital Sonifier model 250 fitted with a model 102C probe. This sonicated resin mixture was placed in the build vat of a commercial stereolithographic printer (Form1 from Form Labs, Boston, Mass.) and used to produce a 1 mm thick×10 mm wide by 50 mm long strip in which the nanotubes appeared to be very uniformly distributed, with no CNT agglomeration visible. The highly-flexible finished test strip was washed with isopropanol and then cured in sunlight for one hour. The conductivity of the test strip was then measured using a four-probe multimeter (R-Chek, EDTM Inc.) to obtain the electrical resistance. This test strip exhibited an electrical resistance of about one megaohm over the entire sample length.

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