Whole cell lysates were prepared in RIPA buffer and equivalent protein amounts (generally 50 μg) were resolved by SDS-Page. Gels were transferred to PVDF membranes and immunoblotted with the indicated antibodies. List of antibodies used in this study: α-Drp1 (Abcam), α-Erk1/2, α-pT202/Y204Erk 1/2, α-HK2, α-HIF1α, α-Tubulin, α-Actin, α-GAPDH (Cell Signaling Technologies).
α hif1α
Manufactured by Cell Signaling Technology
Sourced in United States
α-HIF1α is a primary antibody that recognizes the hypoxia-inducible factor 1-alpha (HIF1α) protein. HIF1α is a subunit of the HIF1 transcription factor complex, which plays a central role in the cellular response to hypoxia.
Automatically generated - may contain errors
Lab products found in correlation
α erk1 2, by Abcam (2 mentions)
α pt202 y204erk 1 2, by Cell Signaling Technology (2 mentions)
α hk2, by Cell Signaling Technology (2 mentions)
α drp1, by Abcam (2 mentions)
α tubulin, by Cell Signaling Technology (2 mentions)
α gapdh, by Cell Signaling Technology (2 mentions)
α actin, by Cell Signaling Technology (2 mentions)
α perk, by Cell Signaling Technology (1 mentions)
α eif2α, by Cell Signaling Technology (1 mentions)
α eef2, by Cell Signaling Technology (1 mentions)
α p44 42 mapk erk1 2, by Cell Signaling Technology (1 mentions)
α β actin, by Santa Cruz Biotechnology (1 mentions)
α p38 mapk, by Cell Signaling Technology (1 mentions)
α tbp, by Abcam (1 mentions)
α parp, by Cell Signaling Technology (1 mentions)
α caspase 3, by Cell Signaling Technology (1 mentions)
α cyclin d1, by Cell Signaling Technology (1 mentions)
α cdk4, by Cell Signaling Technology (1 mentions)
4 protocols using α hif1α
1
Western Blot Analysis of Cellular Proteins
2
ER Stress Responses in Mammalian Cells
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Drugs: GSK2606414 (Cay17376-1); Tunicamycin (Cay11445-10); Thapsigargin (Cay10522-1); CCT020312 (Calbiochem #324879); GSK2656157 (Cay17372-5); CoCl2 (C8661-25G). Primary antibodies: α-PERK (Cell Signaling, 3192S), α-eIF2α (Cell Signaling, 9722S), α-eIF2S1 (ab32157), α-ATF6α (NBP1-40256), α-IRE1α (sc-20790), α-P-IRE1α (Thermo Scientific, PA1-16927), α-EEF2 (sc-166415), α-PAM (ab-109175; recognizing the cytosolic domain), α-HIF1α (Cell Signaling, 3716S).
3
Protein Expression Profiling by Western Blot
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Cells were lysed using lysis buffer (40 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% sodium dodecyl sulfate (SDS)). Approximately 20 μg of the protein extract was resolved via SDS-PAGE and analyzed via immunoblotting with primary antibodies against α-HIF-1α (Cell Signaling, Danvers, MA, USA, 3716), α-PARP (Cell Signaling, 9542), α-Caspase-3 (Cell Signaling, 9662), α-p44/42 MAPK (ERK1/2) (Cell Signaling, 4695), α-Phospho-p44/42 MAPK (Erk1/2) (Cell Signaling, 4370), α-p38 MAPK (Cell Signaling, 9212), α-Phospho-p38 MAPK (Cell Signaling, 4511), α-JNK2 (Cell Signaling, 9258), α-Phospho-SAPK/JNK (Cell Signaling, 4668), α-Cyclin B1 (Santa Cruz, Dallas, TX, USA, sc-752), α-Cyclin D1 (Cell Signaling, 2978), α-Cyclin E1 (Cell Signaling, 4129), α-CDK4 (Cell Signaling, 12790), α-p53 (Millipore, Burlington, MA, USA, 05-224), α-p21 (Cell Signaling, 2947), α-TBP (Abcam, Cambridge, UK, ab818), α-β-actin (Santa Cruz, sc-47778), and α-alpha-tubulin (Abcam, ab7291).
4
Western Blot Analysis of Cellular Proteins
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Whole cell lysates were prepared in RIPA buffer and equivalent protein amounts (generally 50 μg) were resolved by SDS-Page. Gels were transferred to PVDF membranes and immunoblotted with the indicated antibodies. List of antibodies used in this study: α-Drp1 (Abcam), α-Erk1/2, α-pT202/Y204Erk 1/2, α-HK2, α-HIF1α, α-Tubulin, α-Actin, α-GAPDH (Cell Signaling Technologies).
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