An Agilent Series 1100 HPLC chromatograph (Agilent, Technologies, Palo Alto, CA, USA) was used, equipped with a binary pump (G1312A), an autosampler (G1379A), a degasser system (G1379A), diode array (DAD, G1315B), and fluorescence (FLD, G1321A) detectors. The separation was carried out with a Kinetex C18 (150 mm length × 4.6 mm I.D, 2.6 µm particle size).
The LC–MS system used for the tentative identification of polyphenols was an Agilent 1100 Series liquid chromatograph coupled to an Applied Biosystems 4000 QTRAP hybrid triple quadrupole/linear ion trap mass spectrometer (AB SCIEX, Framingham, MA, USA).
A Lambda 19 double-beam UV–VIS–NIR spectrophotometer (Perkin Elmer, Waltham, MA, USA) was used for the spectrophotometric assays. Measurements were performed with 10-mm path length cells (QS quartz glass, Hellma, Müllheim, Germany). The absorbance was recorded at 765 nm for the FC assay and at 595 nm for the FRAP assay.
Complementary laboratory equipment comprised an ultrasonic bath (Branson 5510, Danbury, CT, USA) and the Labofuge 400 centrifuge (Heraeus, Hanau, Germany).
The LC–MS system used for the tentative identification of polyphenols was an Agilent 1100 Series liquid chromatograph coupled to an Applied Biosystems 4000 QTRAP hybrid triple quadrupole/linear ion trap mass spectrometer (AB SCIEX, Framingham, MA, USA).
A Lambda 19 double-beam UV–VIS–NIR spectrophotometer (Perkin Elmer, Waltham, MA, USA) was used for the spectrophotometric assays. Measurements were performed with 10-mm path length cells (QS quartz glass, Hellma, Müllheim, Germany). The absorbance was recorded at 765 nm for the FC assay and at 595 nm for the FRAP assay.
Complementary laboratory equipment comprised an ultrasonic bath (Branson 5510, Danbury, CT, USA) and the Labofuge 400 centrifuge (Heraeus, Hanau, Germany).