Male ApoE−/− mice were purchased from Beijing Vital River Laboratory Animal Technology Co. Ltd (China) and housed in an environmentally controlled room at 22 ± 2°C and 50% ± 5% humidity with a 12 h/12 h light/dark cycle with free access to food and water. They were allowed to acclimate one week before performing intraperitoneal glucose tolerance test (IPGTT) and then were randomly assigned to control group (Chow group) and diabetic group (DM group). Mice in the diabetic group were fed with a high fat diet (20% fat, 20% sugar, and 1.25% cholesterol; Beijing HFK Bioscience company, China). After 8 weeks, IPGTT was performed to confirm the appearance of insulin resistance. Those mice showing insulin resistance were intraperitoneally injected with one low-dose STZ (75-80 mg/kg body weight in 0.1 mol/L citrate buffer, pH 4.5). Two weeks after STZ injection, most mice displayed hyperglycemia, insulin resistance, and glucose intolerance (Supplementary Figure S1 ), as previously reported (Wang et al., 2014 (link)). Mice with similar degrees of hyperglycemia and body weight were randomly divided into DM (DM, n = 20), DM + nc-shRNA (n = 20) and DM + ALK7-shRNA, n = 20), and injected with nc-shRNA or ALK7-shRNA lentivirus at a dose of 4×109 ifu per mouse via tail vein. At the end of experiment, mice were sacrificed and aortas were collected for subsequent analysis.
Male apoe mice
Manufactured by Charles River Laboratories
Sourced in China
Male ApoE−/− mice are genetically modified mice that lack the apolipoprotein E (ApoE) gene. The ApoE gene plays a key role in lipid metabolism, and its absence in these mice results in elevated levels of cholesterol and the development of atherosclerosis, a condition characterized by the buildup of fatty deposits in the arteries.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6, by Charles River Laboratories (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
C57bl 6j, by Charles River Laboratories (1 mentions)
Spf grade, by Charles River Laboratories (1 mentions)
Berberine, by Merck Group (1 mentions)
Antibodies against phospho erk1 2 and total erk1 2, by Cell Signaling Technology (1 mentions)
Anti gapdh antibody, by Abcam (1 mentions)
C57bl 6j male mice, by Charles River Laboratories (1 mentions)
U0126, by Cell Signaling Technology (1 mentions)
D12492, by Beijing Huafukang Biotechnology (1 mentions)
Male c57bl 6 mice, by Charles River Laboratories (1 mentions)
Td 08028, by Inotiv (1 mentions)
Enzymatic assay kit, by Fujifilm (1 mentions)
Dict 500, by BioAssay Systems (1 mentions)
Mouse albumin elisa quantification kit, by Fortis Life Sciences (1 mentions)
D α tocopherol succinate, by Merck Group (1 mentions)
Osmotic minipump, by Durect (1 mentions)
Ang 2, by Merck Group (1 mentions)
C57bl 6j wt mice, by Taconic Biosciences (1 mentions)
21 protocols using male apoe mice
1
Diabetic Mouse Model Development
2
ApoE Knockout Mice in SPF Conditions
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Male ApoE−/− mice of 7 weeks old were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. [certificate number: 11400700352760, permit number: SCXK (JING) 2016–0006]. All mice were housed and bred under specific pathogen-free (SPF) conditions of Nanjing University of Chinese Medicine Experimental Animal Center [permit number: SYXK (SU) 2014–0001]. All animal studies were approved by Nanjing University of Chinese Medicine Experimental Animal Center and were performed in strict accordance with “Principles for Use of Animals” and “Guide for the Care and Use of Laboratory Animals” of the U.S. National Institutes of Health.
3
High-fat, High-cholesterol Diet in apoE-/- Mice
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Male apoE-/- mice on C57BL/6 background were purchased from the Beijing Vital River Laboratory Animal Technology Corporation and bred in-house. Mice were housed under specific pathogen-free (SPF) conditions, and fed a normal laboratory diet. One week prior to drug administration, all mice started a diet containing milk fat (21% wt/wt) and cholesterol (0.15% wt/wt, Beijing Keaoxieli Corp., Beijing, China), which maintained for 5 weeks.
4
Hyperlipidemic Mice Model and BYD Treatment
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Male ApoE−/− mice on a C57BL/6J background (SPF grade), weighing 18~22 g, were purchased from Beijing Vital River Laboratory Animal Technology Co. Ltd. (license no. SCXK (Jing), 2016-0011). All mice were raised in the barrier environment (room temperature of 24 ± 1°C and relative humidity of 50 ± 1% with a 12 h day/night cycle) and adaptability fed 1 week. Then animals were randomly divided into 5 groups (n = 6~9): chow diet control group (CG), HFD-induced model group (HG), the low and high doses of BYD-treated group (BYD-L and BYD-H, respectively), and ezetimibe (Schering-Plough Ltd., USA, lot: 2EZPA17005)-treated group (EG). According to the daily dose of the adult, mice assigned to BYD-L, BYD-H, and EG groups were orally administrated 150 and 300 mg/kg/day of BYD and 10 mg/kg/day of ezetimibe. In addition, in the control group, mice were fed with chow diet; the other groups of mice were fed high-fat diet (HFD) to establish the model of hyperlipidemic mice. The HFD contained 89.8% chow diet, 0.2% cholesterol, and 10% fat. BYD was administered orally by gavage for 6 weeks while maintaining HFD. All animal experiments have followed the guidelines of the ethics committee of Beijing University of Chinese Medicine. And the experimental protocol was approved by the medical animal experiment ethics committee of Beijing University of Chinese Medicine.
5
Male apoE-/- Mice Dietary Study
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Male apoE−/− mice (6-weeks-old; weight, 20 g; n=24) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). The mice were housed in cages (temperature, 22°C) with a 12-h light/dark cycle and allowed ad libitum access to food and water. The protocols of animal experiments were performed following the National Institutes of Health Laboratory Animal Care and Use Guidelines (21 ) and approved by the Animal Ethics Committee at Jilin University.
6
Atherosclerosis Model in ApoE^-/- Mice
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Male ApoE−/− mice (n=24, age, 8 weeks; weight, 20-25 g) were purchased from the Beijing Vital River Laboratory Animal Technology Co., Ltd. Animals were housed in a specific pathogen-free animal facility with 60% humidity at a constant temperature of 24±2°C. To establish the in vivoatherosclerosis model, animals were randomly divided into four groups: i) Control group, containing ApoE−/− mice fed on a standard 4% fat diet (cat. no. D12450B; Beijing HFK Bioscience; ii) atherosclerosis (AS) group, containing ApoE−/− mice fed on a high-fat diet with 60% of total calories derived from fat (cat. no. D12492; Beijing HFK Bioscience) and an intravenous injection of 200 µl PBS per dose; iii) siRNA-ctrl exosome (Exo) + AS; and iv) si-LOC100129516 Exo + AS groups, containing ApoE−/− mice fed on a high-fat diet, with an injection of siRNA-ctrl Exo or si-LOC100129516 Exo via the tail vein twice a week. All mice were sacrificed using CO2 (30% volume/min) and blood samples and arteries were collected for subsequent experiments. All animal procedures were approved by The Committee of the First Affiliated Hospital of Jinzhou Medical University (approval no. FAHJMU20210113). The National Institute of Health Guide for the Care and Use of Laboratory Animals was strictly followed (29 ).
7
Berberine Regulates Hepatic PCSK9 Expression
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Male C57BL/6J mice (n=7, 8-week-old) and male ApoE-/- mice (n=28, 8-week-old) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. Berberine was purchased from Sigma-Aldrich (St. Louis, MO, USA). The human hepatoma cell line, HepG2, was obtained from Cell Resource Center, IBMS, CAMS/PUMC (Beijing, China), and U0126 (ERK1/2 inhibitor) was purchased from Cell Signaling Technology (Beverly, MA). Anti-proprotein convertase subtilisin/kexin type 9 (PCSK9), anti-LDL receptor (LDLR), and anti-GAPDH antibodies were obtained from Abcam (Cambridge, UK). Antibodies against phospho-ERK1/2 and total ERK1/2 were purchased from Cell Signaling Technology (Beverly, MA). The experiments were performed under a project license (No. 0000555) granted by the animal experimental center of Fuwai hospital, in compliance with the guidelines of the Care and Use of Laboratory Animals published by the US National Institutes of Health.
8
Atherosclerosis Modeling in ApoE-/- Mice
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Male ApoE−/− mice (aged 7 weeks) were purchased from Vital River (Beijing, China) and housed in a specific-pathogen-free animal facility. To establish atherosclerosis model in vivo, these mice were fed a high-fat diet containing 60% of total calories from fat [D12492; HFK Bioscience (Beijing, China)] or a standard 4% fat diet (D12450B; HFK Biological Sciences) for 12 weeks. An lnc-KCNC3-3:1 knockdown mouse model was constructed by injecting a lentivirus (Lenti) expressing RNA inference (RNAi) targeting lnc-KCNC3-3:1 (lnc-KCNC3-3:1 Lenti-siRNA1), which was purchased from Genepharma (Shanghai, China). Next, lnc-KCNC3-3:1 Lenti-siRNA1 was injected into the tail vein of mice in atherosclerosis + lnc-KCNC3-3:1 siRNA1 group. Finally, mice were sacrificed for collection of arterial tissues and serum. All animal procedures were approved by the Committee of the First Affiliated Hospital of Jinzhou Medical University. The National Institute of Health Guide for the Care and Use of Laboratory Animals was strictly followed (10 ).
9
ApoE Knockout Mice Atherogenic Diet
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Male ApoE-/- mice on the C57BL/6 background (Beijing Vital River Laboratory Animal Technology Co. Beijing, China) were bred in a house in a pathogen-free facility. Eight weeks old mice fed an atherogenic diet were randomly grouped to receive normal saline (0.1 mL/day, i.g.) or NXT (0.7g/kg/day, i.g.) treatment [24 (link)]. The animals were housed individually in the specific pathogen-free barrier facility at constant temperature (20-22°C) and humidity (45%-55%) with a 12-hour light-dark cycle. Eight weeks later, mice were euthanized for further analysis.
10
Effect of Vaspin Overexpression in ApoE-/- Mice
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Male apoE−/− mice (age, 6 weeks; n=16) and male C57BL/6 mice (age, 6 weeks; n=8) were obtained from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and fed a high cholesterol diet containing 16.6% fat, 10.6% sucrose and 1.3% cholesterol (Shanghai SLAC Laboratory Animal Co., Ltd., Shanghai, China) for 12 weeks. The mice were housed in cages with a 12-h light/dark cycle. The temperature of the animal room was maintained at 23±2°C and the relative humidity was maintained at 55±15%. All of the animals were provided free access to drinking water and food. The mice were divided into two treatment groups, LV5NC (n=8) and LV-mus-vaspin (n=8). Each mouse was injected with 200 µl lentivirus through the tail vein. Body weight and food intake were monitored every 5 days in both groups.
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