The BeyoClick™ EdU Cell Proliferation Assay Kit (Beyotime, Shanghai, China) was performed to assess the proliferation of transfected GC cells based on the manufacturer’s instructions. For quantitative measurement of cell proliferation, EdU incorporation was assessed via DAB staining following the manufacturer’s protocol.
Beyoclick edu cell proliferation assay kit
Manufactured by Beyotime
Sourced in China
The BeyoClick™ EdU Cell Proliferation Assay Kit is a tool used to detect and quantify cell proliferation. It utilizes the incorporation of the synthetic nucleoside EdU (5-ethynyl-2'-deoxyuridine) into the DNA of dividing cells, which can then be detected using a click chemistry reaction.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using beyoclick edu cell proliferation assay kit
1
EdU Cell Proliferation Assay for GC Cells
2
Evaluating Cell Proliferation with EdU Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A BeyoClick EdU Cell Proliferation Assay Kit (Beyotime, Shanghai, China) was used to assess cell proliferation. HuH-6 cells were seeded in 96-well plates at a density of 5 × 103 cells/well for 24 h. After incubation with ALS for 48 h, the cells were treated with 10 μM EdU for 24 h. Subsequently, the cells were fixed in 4% paraformaldehyde and permeabilized with 0.3% Triton X-100 for 10 min. Nuclei were counterstained with Hoechst 33342. Finally, the specimens were visualized under a fluorescence microscope.
3
BeyoClick™ EdU Cell Proliferation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Experiments were performed according to the BeyoClick™ EdU Cell Proliferation Assay Kit (Beyotime Biotechnology, China, Cat. no. C0078S) instructions. After Edu labeling of cells, the culture medium was removed, and 1ml 4% paraformaldehyde was added to fix the cells, and the cells were fixed for 15 min. The fixing solution was removed, and after washing, 1ml 0.1% Triton X-100 was added to each well and incubated for 10–15 min at room temperature. After washing, the nuclei were stained with DAPI. The results were observed and recorded with a fluorescent inverted microscope. Images were acquired under a microscope at 10x magnification.
4
ALKBH5 and ADIRF Impact on LUAD Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The effect of ALKBH5 and ADIRF on LUAD cell proliferation was assessed using the BeyoClick™ EdU Cell Proliferation Assay Kit (Beyotime). After the prescribed treatment according to the manufacturer’s protocol, A549 or H157 cells were coincubated with EdU working solution for 2 h in 96-well plates, followed by incubation with 4% (w/v) paraformaldehyde for 20 min at room temperature for fixation and permeabilization with 0.3% Triton X-100. Next, the cells were mixed with DAPI solution to stain the nuclei (blue). Finally, fluorescence microscopy (CX31-P, Olympus) was used to determine the proportion of EdU-positive cells after magnified imaging and to assess cell proliferation.
5
Cell Proliferation Analysis via EdU
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The EdU staining was conducted using a BeyoClick EdU Cell Proliferation Assay Kit (Beyotime, China) according to the manufacturer’s protocol. Images were captured using an Olympus IX73 microscope and the number of cells was manually counted.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!