Human ncm460 colonocytes

HepG2 cells stably transfected with a luciferase reporter (HepG2-ARE-C8) were kindly provided by Professor Dr. A. N. Tony Kong (Rutgers University, Piscataway, NJ) and Prof. Rong Hu (China Pharmaceutical University, Nanjing). Cells were maintained in modified RPMI-1640 medium (GiBco, Invitrogen Corp., USA) with 10% fetal bovine serum (FBS) (GiBco, Invitrogen Corp., USA) and penicillin/streptomycin in a 37 °C incubator with 5% CO₂. Human NCM460 colonocytes (INCELL, San Antonio, TX) were cultured in Dulbecco’s Modified Eagle Medium (Life Technology^TM, 1645798) supplemented with 10% (v/v) FBS and penicillin/streptomycin.

Human NCM460 colonocytes (INCELL, San Antonio, TX, USA) were cultured in Dulbecco’s modified Eagle medium (DMEM) (Gibco™, 11995065, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% (v/v) fetal bovine serum (FBS) (ExCell Bio, FSP500) and penicillin/streptomycin, in a humidified atmosphere of 5% CO₂ and 95% air at 37 °C.

Human NCM460 colonocytes (INCELL, San Antonio, TX) were cultured in Dulbecco's modified Eagle medium (Life Technology™, 1645798) supplemented with 10% (v/v) fetal bovine serum (FBS) (Gibco, NY, USA) and penicillin/streptomycin, in a humidified atmosphere of 5% CO₂ and 95% air at 37°C. HepG2 cells, stably transfected with the ARE-luciferase reporter (HepG2-ARE-C8), were kindly provided by Professor Dr. A. N. Tony Kong (Rutgers University, Piscataway, NJ). The cells were cultured in modified RPMI-1640 medium (Gibco, NY, USA) with 10% FBS (Gibco, NY, USA) in a humidified atmosphere of 5% CO₂ and 95% air at 37°C.