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The COLO 320 is a cell culture flask designed for the cultivation of adherent cells. It features a surface area of 25 cm2 and a vented cap to allow gas exchange. The flask is made of high-quality polystyrene material and is suitable for use in standard cell culture incubators.

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Lab products found in correlation

Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Sw480, by European Collection of Cell Cultures (1 mentions) Hek293t, by European Collection of Cell Cultures (1 mentions) Hct116, by European Collection of Cell Cultures (1 mentions)

2 protocols using colo 320

1

Colon Cancer and HeLa Cell Viability Assay

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The COLO 320 (human colon adenocarcinoma) and HeLa cell lines used in this study were obtained from the European Collection of Cell Cultures (ECACC). The cells were grown in RPMI 1640 medium (GIBCO BRL, Barcelona, Spain) supplemented with 10% fetal bovine serum (FBS), 100 U mL−1 penicillin and 0.1 mg mL−1 streptomycin under 5% CO2 at 37 °C. Cell viability was determined as previously reported [45 (link)]. The concentration of RIPs causing a 50% reduction in viability (IC50) was calculated by linear regression analysis. Sodin 5 and quinoin toxicity was also evaluated using HeLa cells pre-treated with 100 μM of the pan-caspase inhibitor Z-VAD. The reagent was added to cells 3 h before RIP administration, and the cell viability was determined for different RIP concentrations.
Landi N., Ragucci S., Citores L., Clemente A., Hussain H.Z., Iglesias R., Ferreras J.M, & Di Maro A. (2022). Isolation, Characterization and Biological Action of Type-1 Ribosome-Inactivating Proteins from Tissues of Salsola soda L.. Toxins, 14(8), 566.
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2

Cell Line Authentication and Wnt Signaling Assays

Cited 3 times
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Cell lines were purchased from the European Collection of Cell Cultures (HEK293T and HCT-116 in 2007, COLO320 in 2011; SW480 and DLD1 in 2013). RKO cells were kindly provided by Doug Winton (University of Cambridge; in 2012). All cell lines were authenticated by STR DNA profiling. Upon receipt, cells were frozen, and individual aliquots were taken into culture, typically for analysis within <10 passages. For SW480 and COLO320 cells, truncated APC protein was monitored by Western blot analysis (see Results). Cells were grown and transfected for Wnt reporter assays and indirect immunofluorescence as described (22 (link)). Cytotoxicity assays were done as described (19 (link)). An SW480 cell line with integrated TOP-GFP reporter (25 (link)) was isolated by negative selection and cloning of stable transfectants, and GFP was monitored by fluorescence-activated cell sorting (FACS). Standard inhibitor treatment was for 24 hours (2.5 μM XAV939, or 25 μM CA), unless specified otherwise.
de la Roche M., Ibrahim A.E., Mieszczanek J, & Bienz M. (2014). LEF1 and B9L shield β-catenin from inactivation by Axin, desensitizing colorectal cancer cells to tankyrase inhibitors. Cancer research, 74(5), 1495-1505.
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