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2 protocols using d ribose

1

Sugar Substrate Acquisition for Research

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All sugar substrates: D-ribose, 2-deoxy-D-ribose, D-glucono-1,5-lactone, D-fucose, L-fucose, and L-ascorbic acid were purchased from Biosynth Ltd. (Compton, UK).
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2

Synthesis of Nucleotide Sugars

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D-ribose, D-ribulose, and D-ribitol were purchased from Carbosynth. D-galactose was from Merck Millipore. Cytidine triphosphate, D-xylulose, D-xylose, L-arabinose, doxycycline, and 1 mol/L triethylammonium acetate were from Sigma. CMP-N-acetylneuraminic acid, UDPgalactose, UDP-glucose, UDP-N-acetylgalactosamine, UDP-N-acetylglucosamine, GDP-mannose, GDP-fucose, ADP-ribose, UDP-xylose, and UDP-glucuronic acid were from Sigma or Carbosynth.
Rbo5P was produced as previously described (5 ) . CDP-ribitol was synthesized in vitro from Rbo5P and Cytidine triphosphate with recombinantly produced human CRPPA enzyme from Escherichia coli (kind gift of Dr. W. Yue, Oxford) (5 ) . The internal standard, a 13 Cyeast extract containing [U- 13 C]-UDP-glucose, was a kind gift of Dr. G. Hermann, Vienna.
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