Fibroblast medium: DMEM (ThermoFisher), 10% fetal bovine serum (FBS, Hyclone), 1% non-essential amino acids (ThermoFisher), 1 mM GlutaMAX (ThermoFisher), 1% penicillin-streptomycin (ThermoFisher), 55 μM β-mercaptoethanol (ThermoFisher) and 1 mM sodium pyruvate (ThermoFisher). Naive medium (t2iLGoY)19 (link): 50:50 mixture of DMEM/F12 (ThermoFisher) and neurobasal medium (ThermoFisher), supplemented with 2 mM l -glutamine (ThermoFisher), 0.1 mM β-mercaptoethanol (ThermoFisher), 0.5% N2 supplement (ThermoFisher), 1% B27 supplement (ThermoFisher), 1% penicillin-streptomycin (ThermoFisher), 10 ng ml−1 human leukaemia inhibitory factor (made in-house), 250 μM l -ascorbic acid (Sigma), 10 μg ml−1 recombinant human insulin (Sigma), 1 μM PD0325901 (Miltenyi Biotec), 1 μM CHIR99021 (Miltenyi Biotec), 2.5 μM Gö6983 (Tocris), 10 μM Y-27632 (Abcam). Primed hiPS cell medium (KSR/FGF2): DMEM/F12 (ThermoFisher), 20% knockout serum replacement (KSR) (ThermoFisher), 1 mM GlutaMAX (ThermoFisher), 0.1 mM β-mercaptoethanol (ThermoFisher), 1% non-essential amino acids (ThermoFisher), 50 ng ml−1 recombinant human FGF2 (Miltenyi Biotec), 1% penicillin-streptomycin (ThermoFisher). Primed hiPS cell medium (Essential 8 (E8)): 10 ml of E8 supplement (Gibco) to 500 ml medium basal (Gibco), supplemented with 1% penicillin-streptomycin (Gibco).
Medium basal
Manufactured by Thermo Fisher Scientific
The Medium basal is a laboratory product designed for use in cell culture applications. It serves as a base medium to support the growth and maintenance of various cell types. The product provides the necessary nutrients and components to create a suitable environment for cell cultivation.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (6 mentions)
Neurobasal medium, by Thermo Fisher Scientific (4 mentions)
Dmem f12, by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Glutamax, by Thermo Fisher Scientific (4 mentions)
B27 supplement, by Thermo Fisher Scientific (4 mentions)
Recombinant human insulin, by Merck Group (2 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (2 mentions)
Knockout serum replacement (ksr), by Thermo Fisher Scientific (2 mentions)
β mercaptoethanol, by Thermo Fisher Scientific (2 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions)
G 6983, by Bio-Techne (2 mentions)
Recombinant human fgf2, by Miltenyi Biotec (2 mentions)
Pd0325901, by Miltenyi Biotec (2 mentions)
E8 supplement, by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
N2 supplement, by Thermo Fisher Scientific (2 mentions)
L ascorbic acid, by Merck Group (2 mentions)
Y 27632, by Abcam (2 mentions)
Chir99021, by Miltenyi Biotec (2 mentions)
2 protocols using medium basal
1
Fibroblast and Induced Pluripotent Stem Cell Media Formulations
2
Fibroblast and Induced Pluripotent Stem Cell Media Formulations
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Fibroblast medium: DMEM (ThermoFisher), 10% fetal bovine serum (FBS, Hyclone), 1% non-essential amino acids (ThermoFisher), 1 mM GlutaMAX (ThermoFisher), 1% penicillin-streptomycin (ThermoFisher), 55 μM β-mercaptoethanol (ThermoFisher) and 1 mM sodium pyruvate (ThermoFisher). Naive medium (t2iLGoY)19 (link): 50:50 mixture of DMEM/F12 (ThermoFisher) and neurobasal medium (ThermoFisher), supplemented with 2 mM l -glutamine (ThermoFisher), 0.1 mM β-mercaptoethanol (ThermoFisher), 0.5% N2 supplement (ThermoFisher), 1% B27 supplement (ThermoFisher), 1% penicillin-streptomycin (ThermoFisher), 10 ng ml−1 human leukaemia inhibitory factor (made in-house), 250 μM l -ascorbic acid (Sigma), 10 μg ml−1 recombinant human insulin (Sigma), 1 μM PD0325901 (Miltenyi Biotec), 1 μM CHIR99021 (Miltenyi Biotec), 2.5 μM Gö6983 (Tocris), 10 μM Y-27632 (Abcam). Primed hiPS cell medium (KSR/FGF2): DMEM/F12 (ThermoFisher), 20% knockout serum replacement (KSR) (ThermoFisher), 1 mM GlutaMAX (ThermoFisher), 0.1 mM β-mercaptoethanol (ThermoFisher), 1% non-essential amino acids (ThermoFisher), 50 ng ml−1 recombinant human FGF2 (Miltenyi Biotec), 1% penicillin-streptomycin (ThermoFisher). Primed hiPS cell medium (Essential 8 (E8)): 10 ml of E8 supplement (Gibco) to 500 ml medium basal (Gibco), supplemented with 1% penicillin-streptomycin (Gibco).
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