All the study participants provided personal health history, sleep habits, and lifestyle information. The daily intake of vitamin supplements was assessed by an interviewer-administered questionnaire. Daytime sleepiness was assessed with the Epworth Sleepiness Scale (ESS). Blood was drawn for biochemical analysis after overnight fasting. Plasma glucose, serum triglycerides, and HDL cholesterol were measured with an autoanalyzer (ADVIA 1650 and 1800, Siemens, Tarrytown, NY). Hcy level was measured using a chemiluminescence immunoassay assay. A “high” Hcy level was defined as >14.6 µmol for males and >11.1 µmol for females, corresponding to the 75th percentile of the whole study cohort. The population was also sub-divided into groups based on tertiles for the distribution of Hcy levels (12.34 µmol/L in the highest tertile and 9.88 µmol/L in the lowest tertile) for the subgroup analysis.
Advia 1650 and 1800
Manufactured by Siemens
Sourced in United States
The ADVIA 1650 and 1800 are automated clinical chemistry analyzers designed for in-vitro diagnostic testing. They are capable of performing a wide range of routine and specialized clinical chemistry tests on a variety of sample types. The core function of these instruments is to automate the analysis of clinical samples, providing accurate and reliable results to support medical decision-making.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using advia 1650 and 1800
1
Hyperhomocysteinemia and Cardiometabolic Risk
2
Cardiometabolic Risk Factors Assessment
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All subjects were surveyed for age, sex, marital status, smoking, drinking, and other habits. Blood pressure (BP) was measured in a standardized manner using a mercury sphygmomanometer. Body mass index (BMI) was calculated as weight in kilograms divided by height in meters squared. Waist circumference was measured at the midpoint between the lower rib margin and the iliac crest in a standing position. Blood was drawn for biochemical analysis after overnight fasting. Biochemical data including glucose, total cholesterol, triglycerides, HDL cholesterol, and hsCRP levels were determined using an auto-analyzer (ADVIA 1650 and 1800, Siemens, Tarrytown, NY) at Seoul Clinical Laboratories (Seoul, Korea). HCY level was measured using a chemiluminescence immunoassay assay.
3
Biomarkers and Body Composition Analysis
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All the study subjects were examined about demographic profiles, including health history and sleep problems. The Epworth Sleepiness Scale (ESS) was used for the assessment of daytime sleepiness. Blood glucose, lipid profiles, and hsCRP levels were determined (ADVIA 1650 and 1800, Siemens, Tarrytown, NY, USA) in overnight fasting blood. A high hsCRP level was defined as greater than 1.43 mg/dL for males and greater than 1.15 mg/dL for females, in accordance with the 75th percentile among the entire cohort. Multi-frequency bioelectrical impedance analysis (InBody 720; Biospace, Seoul, Korea) was applied to measure body composition [17 (link),23 (link),24 (link)].
4
Biochemical Measurements in KoGES
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The methods used to process and measure biochemical parameters in the KoGES have been described elsewhere [22] . Baseline fasting plasma glucose and triglyceride levels were measured using enzymatic calorimetric methods with automatic analyzers (ADVIA 1650 and 1800; Siemens, Tarrytown, NY, USA). Using triglyceride and glucose measurements, the TyG index was calculated as In[(triglycerides, mg/dl) × (fasting glucose, mg/dl)/2] [14].
5
Cardiometabolic Risk Factors Assessment
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All the study participants provided information about personal health history, sleep habits, and lifestyle. Blood pressure was measured in a standardized manner by a trained research assistant using a mercury sphygmomanometer. At least 2 blood pressure readings were recorded at 30-second intervals, and the average value was used as a measure of systolic and diastolic blood pressure. BMI was calculated as weight in kilograms divided by height in meters squared. Waist circumference was measured at the midpoint between the lower rib margin and the iliac crest in a standing position. Daytime sleepiness was assessed with the Epworth Sleepiness Scale (ESS). Blood was drawn for biochemical analysis after overnight fasting. Plasma glucose, serum triglycerides, high-density lipoprotein (HDL) cholesterol, and hsCRP levels were measured with an autoanalyzer (ADVIA 1650 and 1800, Siemens, Tarrytown, NY). Insulin level was measured with an immunoradiometric assay kit (INS-IRMA kit; BioSource, Nivelles, Belgium). High hsCRP level was defined as >1.46 mg/dL for male and >1.16 mg/dL for female, corresponding to the 75th percentile among non-OSA controls.
6
Automated Cystatin C Measurement
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Plasma cystatin C was measured by an automated particle-enhanced immunoturbidimetric method on the Advia 1650 and 1800 analysis system (Siemens Healthcare Diagnostics) with reagents obtained from DakoCytomation and according to the procedure recommended by the reagent producer. Precision measured during a 3-month period on two instruments showed a total CV of 4.2% at a cystatin C concentration of 1.2 mg/L and 3.2% at 4.6 mg/L (reference values used, 0.55—1.15 mg/L for persons 1–50 years of age and 0.63—1.44 mg/L for persons >50 years). All samples were analysed within 1 day after collection.
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