Anti flag m2 antibody

Manufactured by Agilent Technologies

The Anti-FLAG M2 antibody is a laboratory tool used to detect and purify proteins that have been engineered to contain a specific FLAG peptide sequence. The antibody binds to the FLAG tag, allowing researchers to identify and isolate the target protein for further analysis or experimentation.

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Lab products found in correlation

Secondary peroxidase labeled anti mouse or anti rabbit immunoglobulin g antibodies, by Jackson ImmunoResearch (1 mentions) Lc3b d11, by Cell Signaling Technology (1 mentions) Gst b 14, by Santa Cruz Biotechnology (1 mentions) Image labtm software, by Bio-Rad (1 mentions) Anti mk2, by Cell Signaling Technology (1 mentions) Hybond ecl nitrocellulose membrane, by GE Healthcare (1 mentions) Anti pt180 y182 p38, by Cell Signaling Technology (1 mentions) Anti hsp27, by Cell Signaling Technology (1 mentions) Anti eef2, by Santa Cruz Biotechnology (1 mentions) Anti gfp, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Merck Group (1 mentions) Anti p38, by Cell Signaling Technology (1 mentions) Las 3000, by Fujifilm (1 mentions) Nupage gel, by Thermo Fisher Scientific (1 mentions) Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions) Pvdf membrane, by Thermo Fisher Scientific (1 mentions)

3 protocols using anti flag m2 antibody

Quantitative Western Blot Analysis of Cellular Signaling

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Cells were harvested, fractionated and transferred onto nitrocellulose membranes as described previously (Kim et al., 2015 (link)). Antibodies to STING (D2P2F), phospho-TBK1 (D52C2) and LC3B (D11) were purchased from Cell Signaling Technology. An anti-FLAG M2 antibody (#200474) was purchased from Agilent technologies. Antibodies to tubulin (B-5-1-2) and TBK1 (AOW9) were purchased from Sigma–Aldrich and EMD Millipore, respectively. Anti-HCMV IE (CH160) antibody was purchased from Virusys. Antibodies to IE86 (12E2), c-Myc (9E10) and GST (B-14) were purchased from Santa Cruz Biotechnology. Secondary peroxidase-labeled anti-mouse or anti-rabbit immunoglobulin G antibodies were purchased from Jackson ImmunoResearch. The signal intensity of protein bands were quantitated using Image Lab^TM software (Bio-Rad Laboratories).

Kim J.E., Kim Y.E., Stinski M.F., Ahn J.H, & Song Y.J. (2017). Human Cytomegalovirus IE2 86 kDa Protein Induces STING Degradation and Inhibits cGAMP-Mediated IFN-β Induction. Frontiers in Microbiology, 8, 1854.

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Western Blot Protein Analysis

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Protein extracts were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on 7.5%–16% gradient gels and transferred by semi-dry blotting to Hybond ECL nitrocellulose membranes (GE Healthcare). The following primary antibodies were used to develop the blots: anti-MK2, anti-p38, anti-pT180/Y182-p38, anti-pT334-MK2, anti-p(S/T)-PKD-substrate, anti-Hsp27, anti-pS82-Hsp27 (all purchased from Cell Signaling), anti-GAPDH (Millipore), anti-eEF2, anti-GFP, anti-hRRP40 (all from Santa Cruz Biotechnology), anti-RBM7, anti-ZCCHC8 (both from Atlas antibodies), anti-pS136-Rbm7 (see above); anti-NOGO-B (Rousseau et al. 2005 (link)), and anti-CBP80 (Andersen et al. 2013 (link)) antibody were described before. The anti-FLAG (M2) antibody was purchased from Agilent. ECL detection was carried out on a chemo-luminescence detector (LAS 3000 FujiFilm).

Tiedje C., Lubas M., Tehrani M., Menon M.B., Ronkina N., Rousseau S., Cohen P., Kotlyarov A, & Gaestel M. (2015). p38MAPK/MK2-mediated phosphorylation of RBM7 regulates the human nuclear exosome targeting complex. RNA, 21(2), 262-278.

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Western Blot Analysis of TMSB4Y and Related Proteins

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Whole-cell protein lysates were prepared using Laemmli sample buffer and resolved by SDS-PAGE using NuPAGE gels (Invitrogen), transferred to a 0.2 μm pore size Invitrolon polyvinylidene difluoride (PVDF) membranes (Invitrogen), and probed with primary antibody followed by incubation with horseradish peroxidase-conjugated secondary antibodies. Antibodies used include anti-TMSB4Y (clone 6G4) mouse monoclonal antibody (SAB1403013, Sigma Aldrich), anti-FLAG M2 antibody (200472-21; Agilent), anti-rabbit IgG HRP-linked antibody (7074, Cell Signaling Technology), anti-mouse IgG HRP-linked antibody (7076, Cell Signaling Technology), anti-GAPDH (D16H11) XP rabbit monoclonal antibody (5174, Cell Signaling Technology), and anti-β-actin (clone 13E5) rabbit monoclonal antibody (4970, Cell Signaling Technology).

Wong H.Y., Wang G.M., Croessmann S., Zabransky D.J., Chu D., Garay J.P., Cidado J., Cochran R.L., Beaver J.A., Aggarwal A., Liu M.L., Argani P., Meeker A., Hurley P.J., Lauring J, & Park B.H. (2015). TMSB4Y is a candidate tumor suppressor on the Y chromosome and is deleted in male breast cancer. Oncotarget, 6(42), 44927-44940.

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